For establishing the sampling method of foliar analysis by which the tea plant was diagnosed, the variations of inorganic constituents (ash, N, P
2O
5, K
2O, CaO, etc.) of tea leaves in the year, region, variety, and development in the first. season were determined.
The annual variation : At the middle of every month, the mature leaves of two varietics (
Benihomare and
Tamamitlori) wcre analyzed. These constituents generally reached high levels in autumn, then declined and kept constant, lcvels throughout winter season.
The regional variation : From ten tea experiment stations in the principal tea-producing prefecturesin Japan, the winter old leaf, spring young leaf, and spring old leaf of
Yabukita variety were collected and analyzed. The regional variation of soil constituents was very large, i. e., the max. values of P
2O
5 and MnO were 60 times and 20 times larger than the min. ones of the respective constituents. On the leaf constituents, the variations of N and ash were small, while those of MnO and CaO were large. It was better to adopt the winter old leaf as. the sample for analyzing N or CaO owing its low regional variation, and for analyzing K
2O or MnO any kind of leaves would be adopted. Significant correlations were observed between N, IMO and P
2O
5 contents of soil and those of leaves. The contents of these three constituents in soil influenced upon those of young spring leaf in the case of the former two, and upon that of winter and spring old leaves in the case of the latter one. The tea plant whose old leaf lost larger N content during the period from winter to spring flushed new shoots which were poor in N in spring. The loss of N above-mentioned seemed to be a factor indicating the nutritional status of the plant. Concerning the content of constituents, N, P
2O
5 and K
2O were most abundantly contained in young leaf, CaO and MnO more in old leaves, and ash least in winter old leaf.
The varietal variation : The three kinds of leaves as the same as in the investigation of regional variation were sampled from twenty tea varieties cultured in the field of our station. The analytical results showed that the varietal variations of N and ash were small and the coefficients of variation did not exceed 10%, whale those of MnO and CaO were large as in the case of regional variation. So many significant correlations among the constituents did not exist as in the regional variation, as there were some varieties having special composition of constituents.
The variation in the development of young leaf : The constituents of young leaf of
Tamamidori variety were determined every five days in the first season. The result indicated that the inorganic constituents such as P
2O
5, K
2O, and CaO were kept at fairly constant levels during a wide range of maturity of leaf. It is unnecessary to pay so much attention for sampling tea leaf as in the analyses of organic constituents.
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