Concentration of four major catechins, caffeine and six major amino acids dissolved in the infusions of Sencha were analyzed.
The four major catechins were (-)-epicatechin(EC), (-)-epigallocatechin(EGC), (-)-epicatechin gallate(ECg), and (-)-epigallocatechin gallate(EGCg) and six major free amino acids were theanine, glutamic acid, arginine, asparatic acid, glutamine and serine.
The infusions were prepared in the usual drinking manners by infusing Sencha three times with 50°C, 60°C, 70°C and 90°C water, for 60 seconds for first infusion and for 10 seconds each for second and third infusions, respectively, excepting free amino acids with 90°C.
Increases were observed in the concentration of both catechins and caffeine as the infusing temperatures rose.
Among catechins, EGC and EC were found comparatively soluble, dissolving rapidly in 50°C water. EGCg and ECg, however, were hardly soluble in 50°C water but became soluble to certain degree in 90°C water.
Caffeine was soluble in some degree even in 50°C water and its overall solubility was close to that of free type catechins.
With the exception of arginine, the major free amino acids were rapidly dissolved in the first and second infusions.
At the same time, analysis was performed on the concentration of the four major catechins, caffeine, six major free amino acids, pectins, potassium, magnesium, calcium and phosporic acid dissolved in the infusions of Sencha which were prepared in a special method by infusing with 5°C water for 10 minutes for the first infusion, 50°C water for 1 minute for the second infusion and 95°C water for 1 minute for the third infusion.
In this analysis, concentration of pectins generally decreased with the progress of infusion regardless of their types, whether they are soluble in water, in salt solution or hydrochloric acids.
Concentration of potassium, magnesium and phosphoric acid also decreased gradually with the progress of infusion, while calcium concentrations in the third infusion was a little higher than that in the first and second infusions. Thus, it was presumed that the rich sweetness and "umami" of the first infusion were attributable to the high concentration of amino acids and pectins, while strong bitterness of the third infusion to the high level of concentrations of gallates.
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