Effects of ethanol on the fractions of teichoic acid and polysaccharides in
hioch-bacterial cell walls were investigated, and the following results were obtained.
1) The component of sugar alcohol in the teichoic acid of
Hiochi-lactobacilli H 34 · H 7 and true
Hiochi-bacilli H 1 · S 33 was glycerol. The components of reducing sugar in the teichoic acid of four strains described above were glucose and rhamnose. But, in addition, only the teichoic acid of true
Hiochi-bacilli S 33 contained galactose. Amino sugar was involved only in the teichoic acid of Hiochilactobacilli that is galactosamine was involved in the teichoic acid of
Hiochi-lactobacilli H 34.H 7, and glucosamine was involved in that from
Hiochi-lactobacilli H 7. The amino acid alanine was contained only in the teichoic acid of
Hiochi-lactobacilli H 7 and true
Hiochi-bacilli S 33. Molecular weights of the teichoic acid from 4 strains were:
Hiochi-lactobacilli H 34 45, 000,
Hiochi-lactobacilli H 7 17, 000, true
Hiochi-bacilli H1 17, 00, and true
Hiochi-bacilli S 33 45, 000. The composition and the molecular weight of the teichoic acid prepared from hiochi-bacterial cell walls were the same in cells cultured in the presence and absence of ethanol. Consequently, it was considered that the teichoic acid was not affected by the presence of ethanol. But the content of teichoic acid in cell wall was decreased by cultivation under the addition of ethanol.
2) The polysaccharide fraction extracted from cell wall of each hiochi-bacterium with trichloroacetic acid contained 2 polysaccharides composed solely of glucose, and fucose, respectively. In addition, there was another polysaccharide composed of mannoe in hetero fermentative
Hiochi-lactobacilli H 34.
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