Previous studies revealed that a representative sake yeast strain, Kyokai No. 7(K7), possesses three loss-of-function mutations,
rim155067insA,
msn4C1540T and Δ
ppt1::Ty2, which enable a high fermentation ability of the strain. Since all three were common and specific to K7 and closely related good strains, such as K6, K9, K10 and their derivative strains among approximately 30 strains tested by each mutation, they were expected to work as efficient DNA markers to differentiate those good strains from others. In this study, a rapid and accurate genotyping method, high resolution melting(HRM)analysis, was employed and optimized for typing
RIM155067ins and
MSN41540, respectively. Typing of these DNA markers was then applied to a broad range of yeast strains(98 strains, 134 stocks)to estimate whether they belonged to the group of K7-realated strains.
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