DL-
p-Fluorophenylalanine (
p-FPA) resistant mutants which produce a large quantity of β-phenetyl alcohol were isolated from Saccharomyces cereviciae A 5-8-1 A (
a leu 1), and their genetic properties were studied. The zygote of
p-FPA resistant mutant of A 5-8-1 A and S. cerevisiae C 4488-1 A (α
leu 2) was not resistant to
p-FPA and did not produce a large quantity of β-phenetyl alcohol. The result of tetrad analysis of the zygote showed that the property of β-phenetyl alcohol productivity was separated to 2: 2, and that the properties of β-phenetyl alcohol productivity and p-FPA resistance were cosegregated.
p-FPA resistant mutants producing a large quantity of β-phenetyl alcohol were isolated from sake yeast Kyokai No.7, and used for sake brewing. The brewed sake contained large amounts of βphenetyl alcohol and β-phenetyl acetate, and possessed a fine aromatic character and good quality. Mutants of Kyokai No.7 resistant to L-canavanine and
p-FPA gave, in test brewing, larger amounts of aroma compounds (
i. e., β-phenetyl alcohol, tyrosol, tryptophol, isoamyl alcohol, isoamyl acetate and β-phenetyl acetate). The use of these mutants for sake brewing will serve various characteristic sake.
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