In the production of sake, 4-vinyl guaiacol(4-VG)is produced by the decarboxylation of ferulic acid by ferulic acid decarboxylase from
Saccharomyces cerevisiae. The inactivation of ferulic acid decarboxylase has been reported to be caused by a single nucleotide substitution(c.160A>T)within the ferulic acid decarboxylase gene(
FDC1). Here, we developed a detection method for the single nucleotide polymorphism within
FDC1 by a polymerase chain reaction-restriction fragment length polymorphism with a mismatched primer. This simple, rapid, and sensitive method is expected to facilitate the discrimination of the 4-VG-producing ability of yeast strains.
View full abstract