This paper explores people’s arousal level influenced by monochromatic light exposure during Morning and its correlation with their different behavioral characteristics. The subjects were exposed to 638nm, 523nm and 465nm monochromatic LED bulb lighting, with irradiances of 73.4μW/cm², 74.2μW/cm², and 76.9μW/cm², respectively. The experiment measured the subjects’ alpha wave ratio, time estimate, KSS(Kwansei-gakuin Sleepiness Scale). MEQ (Morningness eveningness Questionnaire) was conducted to assess the subjects’ morningness-eveningness preference. The methods of ANOVA(analysis of variance) and multiple comparison were employed. Moreover, in order to investigate the correlation between the influence of monochromatic light exposure on people’s arousal level and their morningness-eveningness preference-a significant individual behavioral characteristic, a correlation analysis between the horizontal axis being MEQ scoring and vertical axis being the varying physiology measurements has been made under different lighting conditions.It can be found that during and after the monochromatic light exposure, the alpha wave ratio at Cz under 465nm lighting was significantly higher than that under 638nm lighting at 10:30 and 12:05. And, the KSS scoring under 465nm lighting was significantly higher than that under 638nm lighting at 10:30 and 12:05. Moreover, the time estimate of 180s under 638nm lighting was significantly shorter than that under 465nm at 10:00, 11:00 and 12:00. Meanwhile, under the 465nm lighting, the alpha wave ratio at Cz showed significant negative correlation with MEQ scoring. The results show that the arousal level of the subjects exposed to 465nm lighting was lower. Meanwhile, the evening type subjects showed lower arousal level than the medium type when the arousal level of both types dropped. Thus, people with different morning-evening preferences had different circadian rhythm of Melanopsin’s spectral sensitivity.
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