The heparinized plasma collected from 11 non-treated, 10 agar-injected rabbits and 10 heparinized acidified plasma samples were eluated by column chromatography (G-25, G-50, G-200, DEAE-cellulose and DEAE-Sephadex A-50) or treated by starch-block electrophoresis. Using column chromatography fo G-25, G-50 or G-200, the platelet-clumping activity was found in the protein fractions. Using chromatography of DEAE-cellulose, the platelet-clumping activity was eluated in 1.5 M NaCl containing phosphate buffer, pH 6.0. Using chromatography of DEAE-Sephadex A-50, it was eluated in 0.17 M NaCl containing phosphate buffer following the albumin fraction. Using starchblock electrophoresis, the platelet-clumping activity was found in the albumin band and/or in fractions containing a component of faster mobility than that of albumin (pre-albumin fraction). Platelet-clumping activity was also detected in electrophoretic extracts of plasma from non-treated rabbits which, prior to electrophoresis, had not shown any platelet-clumping activity. Starchblock electrophoresis may serve to activate the platelet-cluming substance or remove some inhibitor in the plasma. The eluates collected through the above mentioned procedures clumped platelets not only in citrated plateletrich plasma but also in platelet-saline suspension without calcium ions. It was not dialyzable against running water for 72 hours; it was heat-stable at 100°C for 15 min.; it failed to demonstrate a peak around 260 mμ in the ultraviolet absorption test.

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1. The toad tenth sympathetic ganglion consisted of two efferent conduction systems according to conduction velocity and threshold, i. e. sB and sC systems.
2. Neurons in the ganglion might be classified into two groups at least with respect to their soma size, i. e. into large and small neurons.
3. It was suggested by the results of marking the neurons intracellularly that the large neurons might compose the fast sB conduction system, and small neurons sC system.
4. The possibility was presented that sB system might innervate the toxic gland of the skin, and sC system the vascular system of the lower limb.
5. Both sB and sC systems were assumed to consist of adrenergic neurons. or catecholamine containing neurons.

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1. Basic and clinical applications of a newly developed apparatus for the measurement of body movement has been made.
2. Some correlations have been found between body weight and height.
3. The pattern of body movement is influenced by visual sensation and the mental state. A man with high anxiety state usually showed a large fluctuation. 4. As the pattern is increased in fatigue, this apparatus can be used as an index of fatigue examination.
5. As the pattern is influenced greatly by alcohol intake, it can be used to determine degree of intoxication.
6. It can be used for psychiatric, ophthalmologic, otologic, and psychological research.

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1. Firing patterns of Ia afferents and motoneuron efferents were recorded while subjecting homonymous de-efferented triceps surae muscle to vibration applied longitudinally to the tendon.
2. The amplitude of vibration (ΔL) necessary to elicit the preferred firing rate of each motoneuron was noted over a wide range (20 to 180cps) of vibration frequencies (f). This permitted the plotting of a ΔL-f curve for each motoneuron, the area above the curve being termed the “response area” and the frequency associated with the minimal ΔL being termed the “optimal frequency” of vibration. In general preferred firing rates ranged from 6 to 13imp/sec and the optimal frequency was near 100cps.
3. These parameters of vibrator responsiveness were measured before and after post-tetanic potentiation. They were also measured before and during concomitant stretch of a synergist and stimulation of antagonistic afferents. The facilitatory effect of post-tetanic potentiation and stretch of the synergistic muscle resulted in a broadening and downward shift of the Δ-f curve to the right. This indicated an increase in response area and shift in optimal frequency to a higher value. Reverse inhibitory effects were noted during stimulation of antagonist afferents.
4. Similar parameters were generated for Ia afferents by studying their vibrator responsiveness at arbitrarily fixed rates of 20 and 10imp/sec. The optimal frequency of vibration was quite similar for afferents (20imp/sec) and efferent, as were the ΔL values associated with this frequency. The further vibration frequency was removed from the optimal frequency the more pronounced became the difference in ΔL requirements, far less amplitude of stretch being necessary to maintain the selected Ia firing rate. There is a discussion of mechanisms underlying the difference in profile of ΔL-f curves for Ia afferents and motoneurons.

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Isometrical contraction tension was studied under varied rates of stimulation in the canine right ventricle, which was perfused with blood from a donor dog. In such preparations, a spontaneous rhythm of about 20 beats/ min was usually observed. Stimulus threshold and tension developed by stimulation with a voltage approximately twice threshold did not change significantly for 10 to 20 hours of continuous driving at constant rate (120/min). Contractile force was increased by raising the frequency of stimulation and a definite and reproducible frequency-force relationship was observed in the range of stimulation rate from 30 up to 200 per minute at a constant temperature of 38-39°C. Atropine, β-adrenergic blocking agent or tetrodotoxin did not modify the frequency-force relationship. It was concluded that such frequency-force relationship as observed in the papillary muscle preparation having sufficient blood supply is the inherent characteristic property of the ventricular muscle. Alternation in contractile tension was observed at rates of stimulation above 200 per minute. The degree of alternans became reduced by catecholamines.

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A rapid scanning spectrophotometer was used for the measurement of the O₂ replacement reaction by CO from O₂Hb solution at a low Pco range. The Hb concentration in the reaction mixture was equivalent to 3, 500-fold-diluted whole human blood. The reaction rate was measured at a Pco of 3.3 and 6.5mmHg and a Po₂ of 100, 200, 343, 499 and 673mmHg, respectively. The most prominent spectrum change was observed at the Soret-band of 420mμ. The rate of Sco change, dS/dt, was linearly proportional to the Pco. The velocity factor, Fs, was independent of Sco, and was not hyperbolic against the Po, as shown by ROUGHTON, FORSTER and CANDER. But Fs was in an exponential relationship to the Po2 as shown by the following equation within an error of ±10%;
exp (Fs) =-10^-3·P_o2-1.68.
The authors are grateful to Prof. Masaji MOCHIZUKI for his intimate guidance and and reviewing of the manuscript. They are also greatly indebted to Mr. Takashi ARAI for the construction and improvement of the stopped flow apparatus.

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1. Interaction of thermal with gustatory stimulations of the tongue was examined by recording impulse discharges in single chorda tympani fibres of rats.
2. When temperature of a taste solution flowing over the tongue was lowered or raised suddenly, a transient change in the impulse frequency was observed: In some fibres cooling produced an increase in the impulse frequency and warming a decrease, while in other fibres a fall in the frequency was brought about by a cold solution and a rise by a warm solution.
3. Changes in the impulse frequency after sudden temperature change of taste solutions depended inversely on the impulse frequency before thermal change but not on cold sensitivity of the unit.
4. Relationships between the magnitude of response to NaCl solutions of varying concentrations and temperature, to which the tongue had been adapted, were determined. In a few fibres the impulse frequency produced by NaCl solutions of 0.003-0.3M decreases monotonically with increasing temperature, while in other fibres it becomes high with increasing temperature up to 30°C and declines above it. In the remaining fibres a rise in temperature results in a decrease in the impulse frequency with NaCl solutions of low concentrations while the frequency increases with a rise in temperature with those of high concentrations.
5. Certain correlations appear to exist between dependence of impulse frequency in response to NaCl on temperature and sensitivity of units to the four basic stimuli.
6. The response magnitude-concentration relationships at various temperatures were found to be expressed approximately by a power function of a form R=KSⁿ. The value of the exponent n becomes high with a rise in temperature up to 30°C but decreases slightly with its further rise, the average value of n for NaCl being 0.8 at 20°C, 1.6 at 30°C and 1.4 at 40°C.

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