In the concealed information test (CIT), the heart rate temporally decreases within 5 s of item presentation (initial deceleration), and subsequently increases rapidly, which is followed by a marked decrease (late deceleration). However, the discriminability of the initial deceleration between crime relevant and irrelevant items has been inconsistent. Therefore, the heart rate data of a mock crime CIT experiment obtained from 23 participants were analyzed and processed by standardizing all data points within a set (S data), and calculating the degree of change from the mean, 3 s before item presentation (C data). The mean values within 3 and 5 s, the minimum value within 3 s, the maximum value between 3 and 7 s, and the mean value between 6 and 15 s from the presentation of items were compared for S and C data. The results showed that the analysis using C data could discriminate relevant and irrelevant items, except for the minimum value, although there were no significant differences between them. The availability and limitation of using the initial deceleration are discussed.