All issues
Volume 47 (2009)
- Issue 5 Pages 665-
- Issue 4 Pages 533-
- Issue 3 Pages 419-
- Issue 2 Pages 215-
- Issue 1 Pages 1-
Volume 47, Issue 4
Displaying 1-14 of 14 articles from this issue
- |<
- <
- 1
- >
- >|
REVIEW
-
Yayoi IDAIRA2009 Volume 47 Issue 4 Pages 533-538
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSWe conducted morphological and immunohistological examinations to determine the disappearance mechanisms of the tooth germ in EL/sea mice. Morphologically, the third molar in tooth germ grew through the bud stage to cap stage and then the growth was arrested by the abnormal cell proliferation. After that it gradually disappeared.In immunohistological observations, it was indicated that a Shh signaling pathway played an important role in the onset of these malformations in tooth germ.Secondly, we examined the essential role of Zip13 for periodontal tissue in Zip13 knock out mice.The gene expression of Zip13 was found in only mesodarmal collagen secreting cells such as fibroblasts. It was revealed that Zip13 plays an important role for periodontal tissue formation in terms of immature to mature fibroblasts cell differentiation and instructions.View full abstractDownload PDF (1104K) -
Mitsuhiro MATSUZAWA2009 Volume 47 Issue 4 Pages 539-549
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSAmelogenin is a major protein component of the enamel matrix associated with the progression of enamel mineralization. While the majority of research into amelogenin function has understandably focused on its role in enamel structure formation, recent work investigated the function of amelogenin as a signaling molecule. The present study examined whether amelogenin activates the Wnt/β -catenin signaling pathway in mouse calvarial osteoblasts and human periodontal ligament (PDL) cells and used the T 7 phage-display osteoblast library to identify a target protein with which amelogenin can interact. We found that amelogenin activates the Wnt/β -catenin signaling to induce osteogenic differentiation of osteoblasts and PDL cells. The DNA sequence that demonstrated one of the highest affinities for amelogenin was the tissue inhibitor of metalloproteinase-2 (TIMP-2). The physiological significance of the interaction between amelogenin and TIMP-2 is that amelogenin inhibits the effect of the TIMP-2 inhibitor on MMP activity. Amelogenin disrupted the TIMP-2 inhibitory effect and activated MMP. This increased MMP activity which facilitated the ability of osteoblasts to form a greater number of mineralized nodules. These findings suggest that amelogenin can induce cell differentiation of osteogenic cells through activation of the signaling pathway and may facilitate the mineralization procedure during matrix formation by increasing the MMP activities.View full abstractDownload PDF (1256K) -
Junko SHIMOMURA-KUROKI2009 Volume 47 Issue 4 Pages 550-554
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSA replacement of proline with leucine at position 132 of the receptor for parathyroid hormone(PTH)/parathyroid hormone-related peptide (PTHrP), i.e., PTH-R, has been discovered in patients carrying Blomstrand's lethal chondrodysplasia. As skeletal deformities in this type of chondrodysplasia appear to relate to compromised receptor binding to its ligand, we examined the possibility that rat PTH-R carrying P 132 L mutation (PTH-RP132L) would result in abnormal intracellular localization and transport, by transfecting cDNAs encoding either wild-type PTH-R or mutant PTH-RP132L.ThePTHRP132L was hardly detected on the cell surfaces, but accumulated within the rough-surfaced endoplasmic reticulum. Furthermore, a remarkably weaker intensity of the band compared with the wild-type counterpart suggests that PTH-RP132L is prone to degradation in the transfected cells. These findings indicate that defective transport of PTH-RP132L to the cell surface would be the molecular basis for Blomstrand's chondrodysplasia.View full abstractDownload PDF (1190K) -
Kan SAITO2009 Volume 47 Issue 4 Pages 555-560
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSAmelogenin is a major enamel matrix protein associated with the enamel mineralization. Recently,it was suggested that amelogenin may affect osteoclast. In this study, the effects of amelogenin and amelogenin containing Enamel matrix derivative (EMD) on osteoclastogenesis and osteogenesis were investigated. In co-cultures with calvaria osteoblasts and purified bone marrow cells, amelogenin inhibited osteoclastogenesis. Amelogenin did not have on effect directly on osteoclasts but rather an action on osteoblasts. Amelogenin inhibited the expression of fibronectin lead to down regulate of nuclear factor κB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF), result in osteoclastgenesis was inhibited. Furthermore, 2-Dimension PAGE of EMD followed by western blot analysis revealed that EMD contained amelogenin and BMP 2/4, but not TGF-β . Binding of amelogenin to BMP 2 through heparin/heparansulfate as well as noggin was confirmed in binding assay. Although amelogenin exhibits no noggin-like antagonistic properties against BMP, amelogenin inhibited the binding between BMP 2 and noggin and high density amelogenin reduced the inhibition of alkaline phosphatase activity by noggin. These results suggest that amelogenin effects on osteoblast not only suppressing osteoclast differentiation, but also reducing the inhibition of the BMP by noggin.View full abstractDownload PDF (953K) -
Ryota NOMURA2009 Volume 47 Issue 4 Pages 561-567
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSInfective endocarditis (IE) is caused by microbial infection of the endothelial surface of the heart,for which blood culture examinations are routinely used to determine the responsible bacterial species when diagnosing IE. However, molecular techniques have been recently developed and applied in clinical cases. In the present study, we analyzed 10 cases of IE and compared the bacterial species detected by two different molecular methods with those isolated from blood culture examinations.The blood culture methods identified the bacterial species in 8 cases, while 1 case was negative and the species could not be identified in the other. On the other hand, multiple species were identified in most of the cases by both molecular methods. In addition, we compared specificity and sensitivity between the two molecular methods using known oral streptococcal species. Although not all oral streptococci correspond to known species, the molecular methods used in the present study were extremely sensitive and able to detect from 1−100 bacterial cells.Our results indicate that the present molecular methods are able to detect bacterial species harboring in heart valves with high sensitivity and that species specified by these methods may have incidentally disseminated into the bloodstream. Interpretation of such results should be performed carefully in clinical situations.View full abstractDownload PDF (816K)
ORIGINAL ARTICLE
-
Part VI : Changes in the maximum force of lip closure of various occlusions from the age of children to adultsToshiaki ONO, Tetsuya AOYAMA, Norihiko MURATA, Taro INABE, Shougo KAMI ...2009 Volume 47 Issue 4 Pages 568-575
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSAn analysis of the force of lip closure in children and the effect on oral function and integration of dentition in the period of growth has been carried out. The aim of this study was to clarify the changing properties of the maximum force of lip closure of normal occlusions and a variety of malocclusions from the age of children to adults, and also to clarify the relationship between maximum the force of lip closure and occlusions. Anterior occlusions and the maximum force of lip closure were evaluated in 533 children of nursery school and elementary school, and 69 male adults. We obtained the results as follows :1.The Hellman's dental stage is a significant factor for the maximum force of lip closure, showing a rising tendency with the dental stage.2.The maximum force of lip closure was affected by occlusion after the IIIA dental stage. The contribution ratio of adults was higher than that of children.3.After the IIIA dental stage, the maximum force of lip closure of open bite and maxillary protrusion was lower than that of normal occlusion, while that of cross bite and deep over bite was higher than that of normal occlusion.The above indicates that a marked relationship between lip closing function and integration of dentition begins to be seen from the stage that occlusion of anterior permanent teeth is established, suggesting that function and morphology are affecting mutually during the period of growth.View full abstractDownload PDF (925K) -
Kazuhiro SANADA, Kenju NAGAHIRO, Katsumasa SUZUKI, Tetsuhide MAKIGUCHI ...2009 Volume 47 Issue 4 Pages 576-584
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSThe aim of this study was to investigate the thermal effects of Nd : YAG Laser (8 W, 2 sec, 5 W, 2 sec) on human deciduous enamel by using Polarizing Microscopy, SEM and Micro X-ray Diffraction. The lased central regions showed a milky white circle. From observation of the Polarizing microscope, there were several layers which showed different interference colors around the central regions and these altered regions extended diffusely to the enamel-dentine junction. Based on analysis of the altered regions using the Micro X-ray diffraction, it was found that the enamel crystals [HAP : Ca10(PO4)6(OH)2] were altered as follows ; at the irradiated central regions, high temperature phases such as α -Ca3(PO4)2 and semi-high temperature phases such as β -Ca3(PO4)2 and α -, β -, γ -Ca2P2O7 were identified. In the surrounding layers, there were gradual changes from the high temperature phases to the semi-high temperature phases, and to the normal HAP.View full abstractDownload PDF (1152K) -
Eiya SHIMIZU, Tetsuhide MAKIGUCHI, Katsumasa SUZUKI, Kenju NAGAHIRO, K ...2009 Volume 47 Issue 4 Pages 585-593
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSThe aim of this study was to investigate the thermal effects of Er : YAG Laser (250 mJ, 2 Hz, 200 pulse, and 150 mJ, 2 Hz, 200 pulse) on human deciduous enamel by using Polarizing Microscopy,SEM and Micro X-ray Diffraction. Under the both irradiated conditions, craters are formed in the lased central regions. SEM images showed that the walls of the crater were composed of many layered structures of the enamel prisms, and micro-cracks were observed around the irradiated surfaces of the crater. From observation of the Polarizing microscope, there were layers which showed different interference colors (e.g. brown, orange) around the irradiated central regions (e.g. black-brown). Based on analysis of the altered regions using the Micro X-ray diffraction, it was found that the enamel crystals [HAP : Ca10(PO4)6(OH)2] were altered as follows ; at the irradiated central regions,high temperature phases such as α -Ca3(PO4)2 and semi-high temperature phases such as β -Ca3(PO4)2 and α -, β -, γ -Ca2P2O7 were identified. In the surrounding layers, there were gradual changes from the high temperature phases to the semi-high temperature phases, and to the normal HAP.View full abstractDownload PDF (1099K) -
Chizuru ARAI, Ayumi MAKIGUCHI, Mitsuro TANAKA2009 Volume 47 Issue 4 Pages 594-599
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSIn order to know the important factors that are related to dental caries in pediatric clinic, data were collected and analyzed. Tests on dental caries such as the saliva test and measurement of caries causing microorganism and dental questionnaire were conducted after a series of dental treatments on seventy-three children in deciduous dentition. The following seventeen caries related factors, meal contents, frequency of taking food and drink, snack food, drink along snack, amount of dental plaque,Streptococcus mutans in plaque, Streptococcus mutans in saliva, amount of stimulated saliva, amount of Lactobacillus species, amount of stimulated saliva, buffering capacity of saliva, fluoride usage,numbers of brothers and sisters, frequency of tooth brushing, parent assisted tooth brushing, child position at the time of assisted tooth brushing, use of flossing and xylitol exposure, were numerically ranked and statistically analyzed. Spearman's rank correlation coefficient and Mann-Whitney U test were used for the statistic analysis among the factors.Statistically significant relations to dental caries experience was found in Streptococcus mutans in plaque and saliva, amount of dental plaque, frequency of taking food and drink and meal contents. In addition to these factors, significant difference was found in the amount of Lactobacillus species and xylitol exposure between children with and without dental caries. On the other hand, the amount of plaque was not dependent on factors that are related to tooth cleaning. And the amount of saliva and the buffering capacity of saliva increased in accordance with the age of the children.View full abstractDownload PDF (726K) -
Keiko HIRANO, Yoshihide OKAZAKI, Emi YOSHIDA, Akira KANAO, Xiaopei DU, ...2009 Volume 47 Issue 4 Pages 600-606
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSPrimary school children (aged 9～12 years old, N＝365) were checked for initial caries (white spot) on their permanent anterior teeth. It was investigated whether there is a difference between the white spot and no white spot group with caries activity test (Cariostat®), buffer capacity test (CAT 21 Buf Test®), saliva flow rate, oral moisture, and existence of caries and filling.The findings are as follows :1.55 children (15.1%) had white spots on their permanent anterior teeth (white spot group) There was no significant difference among the 3 grade group (4, 5, 6 grade) in the prevalence of their permanent anterior white spot.2.There was the significant difference between the white spot students group and no white spot group in Cariostat score (more than 2.5/less than 2) and caries prevalence (no caries/caries) by variant analysis. The rate of saliva flow, oral moisture and the rate of buffer capacity test showed no significant difference in any cut off points.3.There was significant difference between decayed surfaces and Cariostat score in the white spot group. The prevalence of high caries risk children in the white spot group had higher caries prevalence than the low caries risk children in the white spot group.View full abstractDownload PDF (860K) -
Taiki OGAWA, Mie SONOMOTO, Michiharu DAITO2009 Volume 47 Issue 4 Pages 607-614
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSDuring dental treatment, because of various stimulations, fear and anxiety occur in infant patients and cause unpleasant stress. Therefore, evaluating the stress of infant patients during dental treatment objectively and taking measures to reduce the stress are important problems. Also, mothers who accompany infants during dental treatment have a special role and the change in the mothers' emotions influence the infant patients.The alpha-amylase is a mechanism that can be measured to determine stress levels. In this research,we measured the alpha-amylase of the infant patients and their mothers who accompanied them in dental treatment.We reviewed whether or not the stress which the index places in the dental treatment of the children was influenced by the stress of their mothers.The patients were the 36 children from 2 to 12 years old with the Osaka Dental University attachment hospital pediatric dentistry and their 36 mothers. As for the saliva which was gathered before and after treatment, we measured the saliva alpha-amylase activated value.We evaluated stress in dental treatment of the children by taking into account the treatment record and gave a questionnaire to their mothers to check the change of the value after reviewing them.The alpha-amylase activated value of the children decreased significantly (p＜0.05), but the value of their mothers didn't change significantly and the influence seemed to be small.However, there were many rates of “less than 7 years old” and “the preventive treatment” in the group which the change of the alpha-amylase activated value sides with, and was influenced by, the stress of their mothers with juvenile children and when little pain stimulation was suggested.View full abstractDownload PDF (1154K) -
Chieko YOKOYA, Kunihiko SHIMIZU2009 Volume 47 Issue 4 Pages 615-620
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSWe researched and examined the influence on tooth enamel when infants drink ionized drinks with pH of 5.5, 4.2 and 3.8 using the mouse strain C 57 BL/6. The mice teeth showed no morphological abnormality same as the control group (drinking purified water) when the mice ate general solid food and drank the ionized drinks (pH 5.5). On the other hand, the mice teeth showed severe dental attrition with pulp exposure on lower jaw when mice drank pH 4.2 and pH 3.8 ionized drinks. Furthermore the mice drank various types of drinks, ate a high sucrose diet in a powdery state and infected Streptococcus mutans showed dental caries advanced to dentin on 1 st or 2nd molar in all experimental group including the control group. Particularly ionized drinks contained high rates of carbohydrates with pH of 4.3 showed the highest caries score and there were no significant differences of caries scores on the other drinks.We suggested that taking ionized drinks with considerable frequency damages the dental enamel and dental caries is advanced by low pH ionized drink including higher carbohydrates while eating a high sucrose diet.View full abstractDownload PDF (984K)
CASE REPORT
-
Shigehiro MIYAMOTO, Hideo OHNO, Tetsuta NISHIKAWA, Rie MIYAMOTO, Takes ...2009 Volume 47 Issue 4 Pages 621-627
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSTemporomandibular joint disorders (TMDs) are characterized by various symptoms and affect many age groups. TMDs in children are particularly difficult to diagnose and the planning of their treatment is complicated. A new medical care protocol model for pediatric TMDs, the critical path Ohno-Shigeta-Oku model (OSO), was developed by the Ohno Pediatric and Orthodontic Dental Clinic-a medical corporation in Shimonoseki City, Japan-in collaboration with the Department of Pediatric Dentistry at Kagoshima University. The purposes of this study were to survey features of TMDs in children, and to evaluate the validity of OSO. Forty-five TMD patients aged 14.6 ± 2.6 years (8 boys aged 15.5 ± 2.6 years, and 37 girls aged 14.4 ± 2.6 years) from the Ohno Pediatric and Orthodontic Dental Clinic were enrolled in the study between April 2003 and March 2008. OSO was applied to them, and the following results were obtained.1.OSO is effective as a medical care protocol model for determining the need for orthodontic treatment and for determining the effects of TMD treatment in children.2.The chief complaint in TMD patients was malocclusion (15 of 45 patients, 33.3%).3.The most important factor of TMDs was malocclusion (32, 84.2%) ; the second was the social and mental factor (27, 71.1%).The results indicate that OSO is an appropriate medical care protocol model. They also suggest that there is a requirement for professional medical care for TMDs in children in the sound growth and development of oral and maxillofacial units.View full abstractDownload PDF (984K) -
Tomoe ARAI, Shoji TANAKA, Akira SUZUKI, Yukio MURAKAMI, Satoko KOBAYAS ...2009 Volume 47 Issue 4 Pages 628-633
Published: September 25, 2009
Released on J-STAGE: March 11, 2015
JOURNAL FREE ACCESSIncisor cusp (talon cusp) is an uncommon dental anomaly manifested as an accessory cusp-like structure on the crown of anterior teeth. We have encountered six cases of incisor cusps in permanent anterior teeth. Three cases were maxillary lateral incisor teeth. One case was a bilateral maxillary central incisor tooth. One case was a rare mandibular central incisor tooth. One case was also a very rarely mandibular abnormaly incisor cusp in the fused tooth of a mandibular lateral incisor tooth and a canine tooth.A review of literature regarding incisor cusp in a permanent anterior tooth was also made.View full abstractDownload PDF (904K)
- |<
- <
- 1
- >
- >|