A number of reports have shown that the caries susceptibility of children with Down syndrome is lower than that of normal children. However, the factors related to this phenomenon have not been fully elucidated due to their diversity. We compared children with Down syndrome with normal children using biochemical analysis and histological observations before and after acid treatment of the outer layer of deciduous teeth. Mandibular deciduous canines without caries or cracks were extracted from 6 children with Down syndrome and 6 normal children. Atomic concentrations of calcium and phosphorus in the core regions of the enamel prism before and after acid treatment were analyzed using an Electron Probe MicroAnalyzer, with histological observations simultaneously conducted. In addition to subjective comparisons of such factors as enamel prism outlines and orientations, the percentage in the enamel prism core (P/I area ratio) was also calculated. A Mann-Whitney U-test was performed for comparisons between children with Down syndrome and normal children, while Wilcoxon's signed-rank test was used for comparisons before and after acid treatment. The following results were obtained. 1.A comparison of calcium concentrations showed no significant difference between children with Down syndrome and normal children. However, that after acid treatment was significantly lower in the normal children. 2.There was no significant differences between the groups regarding phosphorus concentration. On the other hand, that in normal children was significantly decreased with acid treatment, while the phosphorus concentration in those with Down syndrome showed an increasing tendency. 3.There was no significant difference between the groups for Ca/P ratio, which was significantly decreased with acid treatment in both groups. 4.Enamel prism outlines in children with Down syndrome showed heterogeneous patterns, with some incompletely formed. Findings for the enamel surface revealed a three-dimensional structure in normal children as compared to smoothness in children with Down syndrome. Furthermore, the rod sheath of enamel contained an organic matter-like structure in the children with Down syndrome and the prism core was similar to the interprismatic substance in terms of crystal structure. 5.The P/I area ratio before and after acid treatment was significantly lower in children with Down syndrome. The concentrations of calcium and phosphorus, the main components of enamel, were not significantly different between the groups. However, because of the different reactivity to acid, the histological structure in children with Down syndrome did not have a three-dimensional appearance like that seen in the normal children. Our findings indicate that calcification of deciduous teeth in children with Down syndrome is insufficient due to a lack of breakaway of organic materials.
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