In a previous experiment^*, several strains of tubercle bacilli of. the human type which were different from each other in their virulence were injected seperately or jointly into the anterior cham-ber of the rabbit's eye, and it became known that their pathogene-city becomes more intensified, when injected jointly than when inoculated seperately.
In the present experiments, the BCG strain together with a virulent strain of human type, both mixed in various ratios, was inoculated into the anterior chamber of rabbit's eye, and the tuber-culous changes developed in the eye and in other organs were observed.
The arrangement of the experiments and results obtained are as follows:
(1) The pathogenecity of the tubercle bacilli studied was compared by inoculations in the 5 following combinations:
I) 0.005mg. tubercle bacilli of human type.
II) 0.0005mg. tubercle bacilli of human type.
III) 1.0mg. BCG.
IV) 0.005mg. tubercle bacilli of human type+1.0mg. BCG.
V) 0.0005mg. tubercle bacilli of human type+0.1mg. BCG.
(2) The grade of tuberculous changes in the eye runs paral-lel with that in other organs. The pathogenecity was found the highest in IV, next in V, far lower in I and II, and the lowest in III.
(3) It was thus recognized that the pathogenecity of tuber-cle bacilli is more intensely displayed by the mixed inoculation than by the seperate infection.
(4) It is noteworthy that a quantity of BCG inefficient to cause any decided change, when inoculated by itself, shows a conspicuous pathogenecity, when inoculated jointly with an insig-nificant amount of virulent human strain of tubercle bacilli.
(5) It may be concluded, therefore, that the BCG strain, when inoculated together with a virulent strain, displays a marked pathogenecity rather than a preventive effect.

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Durch Immunisierung von koreanischen Rindern mit homo-logem Blut bzw. Milz gelang es, Immunisohämagglutinine und Immunisohämolysine zu erzeugen. Die beiden Antikörper reagier-ten aber an verschiedenen Blutproben nicht parallel, sondern an-sheinend ganz selbständig. In der ersten Mitteilung hat einer der Verfasser (N.) über die agglutinierende Wirkung der Immunseren und über das damit in Beziehung stehende Blutmerkmal "X" na-heres geschrieben. Die in dieser Mitteilung berichtete Arbeit en-thält Untersuchungen über die Isolysereaktion, besonders über die mit ihrer Hilfe vorgenommen individuelle Differenzierung von Rin-derblutzellen. Die Nachstehenden sind die Schlussfolgerungen der Arbeit:
(1) Ein Beweis fur isolytische Fähigkeit der Normalseren von koreanischen Rindern ist nicht geliefert worden.
(2) Die Sera von 15 Rindern, die durch mehrmalige subku-tane Injektion von Rinderpestvirus enthaltenem Rinderblut bzw. Milz hyperimmunisiert worden waren, bewiesen sich ausnahmslos isolytisch aber nicht autolytisch. In solchen Seren wurden aber verschiedene Blutproben nicht gleich stark, sondern in sehr wech-selnden Graden hämolysiert. Wahrscheinlich müssen dafur indivi-duelle Unterschiede der Blutzellen sowie der Immunseren verant-wortlich sein, obgleich hieraus wegen sehr komplizierter Verhalt-nisse keine gesetzmässige Vorstellung erfasst werden kann. (S. Tabelle I).
(3) Serum M, ein Gemisch von 6 stark isolytisehen Immun-seren, hämolysierte die 39 untersuchten Blutproben ausnahmslos. Wenn das Serum aber mit einer beliebigen Blutprobe absorbiert wurde, wurde seine isolytische.Fähigkeit nur gegen das zur Absor-ption benutzte Blut vollkommen aufgehoben, während es gegen die anderen Blutproben fast unverändert blieb oder nur teilweise abge-schwächt wurde (S. Tabelle II und III). Auf diese Weise liessen alle 39 Blutproben sich voneinander deutlich differenzieren. Die Reaktion ist völlig individuellspezifisch.
Die Ergebnisse der Absorptionsversuche mit den einzelnen Immunseren zeigten nicht so regelmässige Resultate wie die mit dem Serum M. Einige Sera wurden durch Absättigung mit ge-wisser Blutprobe nicht nur gegen dieselbe, sondern auch gegen einige andere inaktiviert, und noch einige andere Sera waren mit gewisser Blutprobe schwer absorbierbar, während die meisten Sera sich in einer beschränkten Grenze ähnlich wie das Serum M ver-hielten. (S. Tabelle II).
(4) Drei normale Kãlber wurden mit Blut von drei anderen normalen Kälbern hyperimmunisiert. (S. Tabelle VI). Die Sera aller Immuntiere erwarben schon nach der ersten Immunisierung, d. h. nach der Immunisierung mit Blut einziges Tieres, eine allge-meine isolytische Fähigkeit, so dass die Immunsera fast alle unter-suchtcn Blutproben hämolysierten. (S. Tabelle VII).
Bemerkenswert war dabei, dass das Immunserum die homolo-gen Blutzellen nicht besonders stark hämolysierte. Bei einigen Fällen reagierten die Sera sogar stärker mit gewissen anderen Blutproben als mit den homologen Blutzellen.

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Antigenic properties of lymphatic glands of normal and rinder-pest cattle were again studied by means of complement-fixation test. In this study, 4 hyperimmunized sera were used, namely, -(1) cattle serum against normal lymphatic glands, (2) cattle serum against pest lymphatic glands, (3) rabbit serum against normal lymphatic glands and (4) rabbit serum against pest lymphatic glands. The cross reaction between these sera and both raw and boiled extracts of normal and pest lymphatic glands wese tested. The results sbtained are summarized as follows:
(1) Sera of rabbits and cattle which had been hyperim-munized with normal lymphatic glands, reacted positively with raw extract of either normal or pest lymphatic glands. These sera having been used, there was no difference observed in antigenic property between normal and pest lymphatic glands. The extracts, when boiled at 100°C, however, lose their antigenic action.
(2) The reaction caused by the immune cattle serum belongs to an immune iso-reaction. The question as to whether it exhibits a strict organ specificity excluding the species specificity entirely, however, remains still undetermined. But, judging from the fact that all the antigens tested showed no decided individual differ-ences in their activity, individual specificity may be excluded from consideration.
In this reaction, the cattle immune serum does not lose its activity, when it is heated at 55°C.
(3) As it was shown in previous papers, serum of cattle, hyperimmunized with pest lymphatic glands, reacts specifically with boiled extract of pest lymphatic glands, while it does not react with boiled extract of normal lymphatic glands.
The serum of rabbit, hyperimmunized with the same material, on the contrary, fails to react specifically with pest lymphatic glands. So it behaves quite similarly to the serum obtained by hyperimmunization with normal lymphatic glands. It is very inter-esting that the pest lymphatic gland can produce the pest-specific antibody in cattle while it fails to do so in rabbit.
(4) It is presumed that the reaction between raw extract of pest lymphatic glands and its homologous cattle serum may be a complex of 2 simple reactions, namely, the one specific to rinder-rest and the other specific to tissue antigen. Though the author did not yet succeed in separating the 2 antigens from the extract and the 2 antibodies from the immune serum, such a presumption is justified when the reactions in question are quantitatively studied.

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Since Dr. Hatori (1920) discovered the Formosan chicken mite (Trombicula gallinarum) from the chicken, kingfisher, goatsucker and raven, the present author observed the species from several chickens and sparrows too.
The species have five setae on the scutum in addition to the pseudostigmatic organs which are strongly capitate and each chelicera bears but a single dorsal tooth near the apex, and the palpal claws are trifurcate. Therefore, the present author believes the species belongs to the genus Neoschöngastia Ewing, 1929.
Neoschöngastia gallinarum (Hatori, 1920).
syn. Trombicula gallinarum Hatori, 1920.
Body oval; striations prominent, slightly crenulated; pitting on scutum, epistome, hypostom, legs and palpi. Fangs of chelicerae stout, sharply pointed; dorso-apical tooth minute, very sherp, directed backwards, ventral tooth absent; maxillary setae plain. Palpi rounded; first and second palpal setae with a few barbs; on fourth segment three setae with a few barbs, appendiculum with four pectinate setae; palpal claw trifurcat. Scutum broader than long (mean L 39 μ; W 70 μ; pseudostigmata 39 μ apart), trapezoidal, anterior and lateral margins convex, posterior margin rather sharply concave in centre, corners somewhat angular anteriorly, rounded posteriorly; scutal setae five in number, strongly pectinate, anterior pair the longest, median anterior the shortest. Pseudostig-mata forward to mid-line betweeen scutal setae, small; pseudostig-matic organs capitate, flask-shaped, the stem moderately long, the enlarged head clothed in minute setules; ocular shield placed its own close to scutum, level with the pseudostigmata, anterior eye the larger. Body setae stout, strongly pilose to pectinate, eighty-four to eighty-six in number; on dorsum forty-four, arranged ap-proximately in row as follows: 12:8:8:6:8:2; on venter the anterior setae shorter and more strongly pectinate than the posterior. Fore-leg, mean L 256 μ; leg setae mainly pectinate or plumose, coxal setae single, strongly pectinate or plumose. Length 0.245 (unengorged) to 0.324 mm. (engorged); width 0.152 (unen-gorged) to 0.245 mm. (engorged).
Type host: Gallus gallus domesticus Briss. Localities: Formosa Hayashidamura, Horin, Yoshinomura, Karenko, Taito, Dakusui, Sozan, Chohokuto, Robai, Hokuto, Shirin, Taihoku, Shinten, Tai-heirin, Sekitei, Mokusaku, Seifuku, Shinchiku, Simpo, Kansai.). Type and nineteen paratypes in Taihoku Imperial University Mu-seum, Taihoku. Taiwan (Formosa).
Described from numerous specimens from the skin under the wings of the type host. Also found on Passer montanus tivanensis HARTERT, Taihoku, 4. iii. 26 (by the writer), Caprimulgus affinis monticola FRANKLIN, Corvus coronoides colonorum SWINHOE (Corvus macrorhynchus SEEBOHM of Dr. Hatori), Alcedo atthis japonica Bo-NAPARTE and Centropus bengalensis lignator SWINHOE (Centropus javanicus DUMONT of Dr. Hatori) (by Dr. J. Hatori).

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On February 20, 1935, we found a large malignant tumor in the liver of a foetus of a mare which has aborted in about the ninth month of pregnancy, and the tumor was diagnosed as primary carcinoma originated from epithelium of the bile-duct.
The outlines of the pathological changes of the foetus were as follows.-
The foetus is emaciated, but the lower part of the neck and the abdomen are enormously distended, and the circumference of the latter attains about 97 cm.
The visible mucous membranes are slightly yellowish in colour.
The abdominal cavity contains about 15000 c.c. of turbid brownish red fluid. The abdominal and thoracic cavities communicate by means of a rupture in the lower part of the diaphragm.
The distended part of the neck harbors a cavity which contains about 1000 c.c. of turbid brownish-red fluid, and the cavity communicates with the thoracic cavity by a fissure at the anterior aperture of the thorax.
The liver is markedly swollen and adherent to the right kidney and to the diaphragma. It weighed 3190 gm.. The right lobe includes a tumor about the size of child's head and is some-what prominent on the surface. On transverse section of this lobe, the tumor is irregularly round, 15 cm. in diameter, and shows pus like discharge, in which some small, yellowish granules can be found.
The tumor is very soft, of spongy consistency, granulated and yellowish-white in colonr. The left and the middle lobes are also swollen, congested, dark greenish-yellow in colour, and haemorrhagic patches are scattered on their surface. The section of these lobes shows fine network, dark greenish in colour, and the same coloured fluid can be pressed out.
Microscopic appearance.
The essential elements of the tumor are epithelial cells with large deeply staining nuclei, very scanty cytoplasma, and the cells resemble more or less the epithelium of bile-ducts.
On the periphery of the tumor, the tumorcelles are arranged in alveoli of irregular shape and size, and the meshwork of the stroma is abundant and composed of dense fibrous tissue.
At the centre of the tumor, the connective tissue is meagre in amount and there are many granular substances which are deeply stained by haematoxylin, and necrotic or haemorrhagic areas are also observed.
The remaining part of the liver is in general congested, inter-stitial tissue is developed abundantly around each lobule (mono-lobular cirrhosis) and the cells of the liver parenchyma are small and irregular in shape.
Namely, the tumor is very malignant and embryonic in type, and it may be considerd as a carcinoma originated from the epithelium of the bile-duct.

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