1. Sarcosporidiotoxin contains hemolytic and hemagglutinative principles.
2. The optimal concentration of the erythrocytal suspension to test both of these reactions is 2.5-5.0%.
3. The physico-chemical characteristics of these two principles of the toxin: Hemolytic principle is hardly soluble in water, saline and glycerinated saline, but the hemagglutinative principle is soluble in these media; the one is soluble in alcohol and ether, but the other insoluble; The one is thermostable, but the other is destroyed by heating at 85°C. for 1/2 hour; The one is negative to protein reaction test, but the other positive; Concerning their resistances against acid and alkali, dialysis and adsorption, both of them almost agreed with each other.
4. The normal serum acts indifferently up to a certain amount but above this limit, it rather inhibits hemolysis by the hemolytic principle of the Sarcosporidiotoxin.
5. Alcohol soluble fraction of the serum (or lecithin fraction) accelerates hemolysis by this toxin, but the insoluble fraction (or albumin fraction) and globulin fraction inhibit hemagglutinative action of the toxin.
6. It might be justifiable to conclude from the experimental data that the hemolysis inhibitory substance (albumin fraction) acts directly against hemolytic principle while the hemagglutination inhibitory substance (globulin fraction) first combines with the erythrocytes and consequently inhibits hemagglutination.
7. All the batches of the immune sera prepared with the sarcosporidiotoxin treated by divergent methods were indifferent to hemagglutination.
8. The serum of the rabbit immunized with the alcoholic extracts of the sarcosporidiotoxin, either simple or in mixture with the pig's serum, was indifferent in hemagglutination and hemolysis, but it neutralized the toxic principle.

View full abstract

The intraplantar inoculation of guinea-pigs was tried by the author with virus fixe, as done by a number of investigators with viruses of herpes and foot-and-mouth disease. The results obtained are summarized as follows:
1. Inoculated into the planta with virus fixe, the guinea-pig would die from the symptom and course specific to rabies and contain the virus in its brain. The virus fixe thus can be passed through guinea-pigs by intraplantar inoculation.
2. The virus after 5 plantar passages could not be apparently distinguished from the original virus fixe in the neurotropism. The decided opinion regarding this problem, however, must be established from the result of subsequent experiments.
3. Animals resisting the intraplantar inoculation of virus fixe remain always resistant to similar treatments. This may be due to the development of local immunity following the first inoculation.
4. By repeated intraplantar inoculations, the guinea-pig would become immune to the subdural injection of the original virus fixe. Out of 9 animals subjected to 3 treatments, only 1 case was found susceptible to the subsequent subdural inoculation. Such a well developed immunity of central nervous system against virus fixe seems never to have been observed in any of the hitherto known antirabic treatments.
5. In guinea-pigs repeatedly treated, if they succumbed to the subdural inoculation of virus fixe, the interval between dates of inoculation and death tends to be prolonged. This may also prove the limited development of antirabic immunity in the central nervous system.
6. Such immunity of central nervous system seems to disappear in a half year. The conclusion regarding its duration must be, however, positively determined by the further experiments.
7. Virulicidal principles are detected in the serum of guinea-pigs thus immunized against virus fixe.
8. The guinea-pig is also susceptible to the intraplantar inoculation of "street virus."
9. The author failed to detect the living virus in the brain of 1 guinea-pig, which succumbed to the subdural injection of virus fixe carried out 13 days after 3 intraplantar inoculations. It may probably be one example of "neuro-infection auto-stérilisable" (Levaditi).

View full abstract

[in Japanese]

View full abstract

The author found a female elephant louse on an Indian elephant at Maruyama Zoological Garden in Taihoku last January. That is closely related in most important points to Haematomyzus elephantis which was found by Piaget.
Only differences between both specimens are as follows:
1. There are four double hooks on the posterior part of proboscis tip, instead of three in Piaget's specimen.
2. Four ridges are formed lengthwise on the surface of occiput, while Piaget's specimen has three ridges in the same place.
3. There are brownish shieldform or heartshaped blotches along the margins of six anterior segments of the abdomen. Piaget's specimen has subquadrangular blotches.
4. There are eight rows of nipple-like oval process on the ventral surface. The first and second rows are located on the thorax and connect in their both ends. The Processes on the first row are nine in number, and on the second row are eighteen.

View full abstract