1) The passage of the sheep-pox virus was made through goats in 15 successive cutaneous inoculations with the almost similar eruptions in every generation and we found neither gradual increase of the virulence against goat as it was observed by Konew in his experiment of subcutaneous inoculation on the goat with sheep-pox virus nor gradual decrease of the eruptibility to the goat during certain generations in the primary stage as it was observed by the author in the present experiment, carried out with sheep in successive cutaneous inoculation with the cow-pox virus.
2) The animals employed for the passage of the sheep-pox virus maintain not only perfect immunity against the introduced virus but also remain refractory to the revaccination with sheep-pox virus theretoafter, yet being apparently susceptible to cow-pox virus. Moreover these caprinized sheep-pox virus produce typical eruptions on the normal sheep while no reaction is observed with the sheep immunized previously against sheep-pox. The sheep-pox virus seems, therefore, to be stable with its immunogenic property even after caprinization inoculating cutaneously through many goats.
3) The sheep-pox virus passed through goats by cutaneous inoculation, shows a remarkable decrease in its virulence against the sheep, i. e. no symptom of sheep-pox was observed by injection of the virus by any route, cutaneous, subcutaneous or intravenous, nor it recovers the virulence by inoculating into sheep, even in succession. Thus the author introduced a new vaccine against sheep-pox which has the same character as that of the cow-pox virus against variola, being biologically attenuated and fixed in the virulence against sheep without any change of the original immunogenic property during this procedure.
4) Two out of 17 sheep under immunization with the author's Caprina were kept among the animals infected with sheep-pox and were fonnd insusceptible after all, and the following artificial inoculation with sheep-pox virus upon the all animals resulted in no eruptions on one half the number while another have taken but they were very mild, reminding that the vaccine are potent enough to prevent the spontaneous infection of sheep-pox, although its immunogenic property is naturally much more attenuated than that of the original virus. Comparing the author's own Caprina with Konew's, the former seems to be more ideal as the prophylactic vaccine against shee-pox than the latter.
5) The preventive effect of this Caprina against sheep-pox shows no remarkable differences between the strains derived from the goats and those obtained by goat-sheep method, nor we found any bifference of the effects due to the different method of injection, although this may still be beyond the determination with this minor number of the test animal. Other facts will be claimed on the futher investigation.

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I. In two horses fed on barley only developed in a course of about five months changes as generally observed in the case of osteornalacia.
II. Clinical symptoms appeared more seriously in the younger horse. The principal symptoms were as follows:
a. At first, the symptom of licking appeared, then that of gastro-enteritis catarrhalis, accompanied by depression of appetite.
b. The nutrition and the body weight of the animals gradually decreased during the experimental period.
c. The bones, especially, the facial bones (the lacrymal bone and upper jaw bone) swelled up slightly.
d. Painful condition of the organs of locomotion (pain caused by compression of the vertebral column and hip joint, arched dorsal line, stiff step etc.) were observed now and then; relaxation of the joints and crepitation of the knee-joint continued to appear till the end of observation period.
e. The number of leucocytes, especially, the neutrophile polynuclear leucocytes increased accordingly as other symptoms developed. The basophile leucocytes seemed to increase in the earlier stage of the bone myelitis. No decision could be reached as regards the decrease of the number of lymph-corpuscles.
f. The amount of urine increased in both cases, showing acid reaction.
g. The amount of phosphoric acid (P₂O₅), contained in the urine increased accordingly as the general conditions of the animals became worse, but the quantity of P₂O₅, in 100 c. c. of the urine changed occording to its daily amount, that is, the more the amount of the urine, the less the quantity of P₂O₅.
III. The pathological changes were found in an agrcement with the clinical symptoms, the changes being more serious in the younger horse. The chief changes were as follows.
a. Swelling of bones was slight, but the bone tissue was found to be very soft and weak.
b. Complicated phenomena of resolution, absorption, new fermation and proliferation of bone tissues were observed in the spongy calcareous trabeculae of the ribs. The cardinal changes were such as strictures, swells, or breaks of the trabeculae, multiplication of the homogenous osteoid tissues or of young connective tissues on the margin of the trabeculae, enlargement or disappearing of the lacunae, erosive processes by osteoclasts, and softening and haemorrhage of the bone marrow.
Based upon the results above mentioned, I may incline to think that all the changes noticed in the experimental osteomalacia in the horse fairly agree with those of the natural type of the disease, and this may principally be due to deficiency of calcium salts in the diet.

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The tuberculin-hypersensitiveness is now a generally known fact in the medical world, and it is applied, especially in the veterinary service, for the early detection of the tuberculosis, the true nature of it being obscure yet. Of course, many of scholars earnestly studied to explain the nature, and some of them said that the tuberculin reaction is caused as the reaction between the antigen and the antibody, while some others consider it as a mere increased sensitiveness or as a step toward the immunity against tubercle bacilli. I, also, attempted to explain the nature of this phenomenon expreimentally, employing the rabbit as experimental animals, and I selected the ophthalmic reaction as the best method. Followings are the results I reached from the experimental data.
I) Not only living tubercle bacilli, but also, dead (heated) bacilli, as Kraus & Volk, and others said, have the ability to hypersensitize actively the animals against tuberculin. We are not able completely to devoid off the special antigenic property by heating the bacilli at the boiling point.
II) The blood or the serum from the tuberculous and at the same time tuberculin-hypersensitive rabbits makes passively the same kind of animal tuberculin-hypersensitive, this special quality of the blood or the serum being destroyed at 100°C., and this quality can be taken off by absorption of the blood or serum with living or dead (heated) tubercle bacilli completely, therefore the special substance in the tuberculous blood is not tuberculin, but, on the contrary, antibody in its nature.
III) Passive tuberculin-hypersensitiveness is established 24 to 48 hours after the serum-injection, and disappears in 2 or 3 days.

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