1. The change in the density of hydrogen ion in the blood of an intoxicated rabbit, after the inhalation of carbon disulphide gas.
The alkaline reaction in the blood of animals is generally so weak, that it may be regarded as neutral. In living animals, while differing slightly in each animal, the density of hydrogen ion remains constant during the life of the animal. But it is well known that, in the case of suffocation which causes the augmentation of carbonic dioxide in the blood, the density increases.
The following experiment deals with the difference in density, before the carbon disulphide intoxication, and after cessation of the respiration while the heart beat still continues. In the experiment, the hydrogen elektrode of the potentiometer known as the K type made by Lees and Northrup Co., U. S. A., was used. 1c.c. of blood was taken from the jugular vein of a rabbit into an injection-syringe of 5c.c. capacity, containing a minute quantity of hirudin and 4c.c. of 0.9% physiological sodium chloride solution. They were well mixed in the syringe, and the mixed solution was introduced into the hydrogen elektrode, and examined in a temperature of 20°C.
Thus the average result from the eight cases of the same experiment was obtained: -Before the intoxication, the density of hydrogen ion in the blood was Ph 7.59 and immediately after cessation of the respiration caused by the inhalation of carbon disulphide gas, the density was increased to Ph 7.16, and their difference Ph 0.43 show the increase in the density of hydrogen ion in the blood of the intoxicated animals.
2. Toxic action of carbon disulphide on the isolated heart of a Japanese toad (bufo japonica).
When a rabbit inhalates carbon disulphide gas causes the cessation of respiration, while the heart continues to beat fora few minutes longer. From this, it may be concluded that, carbon disulphide is less poisonous for heart than other well known heart toxins, such as chloroform and carbon tetrachloride, although they resemble each other in chemical composition.
The following experiment was performed to compare the toxic action of carbon disulphide on the heart with that of chloroform and carbon tetrachloride. For this purpose a special heart irrigator, made by Prof. Dr. K. Nakajima of Aichi Medical College was used. The apparatus was arranged as follows: -On one side the heart motion was marked upon the rolling cylinder, and on the other side the carbon disulphide gas was introduced into the Ringer solution in the heart irrigator from the gas washing bottle, by means of the negative pressure, of which mention has already been made in the second and third reports.
As soon as the carbon disulphide gas was introduced into the Ringer solution in the heart irrigator, the heart beat became irregular and weak, and often stopped; then it grew slower and finally ceased altogether. In most cases the whole course continued for from 1 to 10 minutes. On the contrary, when only air was introduced into the Ringer solution, the heart continued to beat normaly for more than one hour.
When the carbon disulphide gas was introduced into the Ringer solution in the heart irrigator, it was condensed into minute drops which sank to the bottom of the irrigator; and according to the heart beat these drops went in and out of the heart. This result having been obtained, 5/10c.c. of carbon disulphide was introduced instead of the gas. This was divided into small drops by the motion of the Ringer solution caused by the heart beat, and passed in and out of the heart. In the last case the toxic action appeared a little sooner than when the gas introduced, but the symptoms were exactly the same in both cases.
When chloroform or carbon tetrachloride was used, the heart beat stopped immediately, but it continued for some minutes after carbon disulphide was used.

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In the previous report (Journal of the Japanese Society of Veterinary Science, Vol. IV, No. 1) the author called attention to the precipitin reaction regarding the differential diagnosis of bovine contagious pleuro-pneumonia. In the experiment which he has made later on, he used as precipitin the serum obtainee from affected cattle or calves infected by inoculation with the virus, and found that the precipitin serum prepared in this way was not fit for use, because the normal cattle serum may sometimes contain precipitin. For this reason he intended to get a precipitin serum from rabbit and found it very suitable for the object.
Preparation of precipitin serum: From a horse-serum-broth-agar slant culture of the virus kept for 10 days in an incubator at 70°C., an emulsion was prepared with 20c.c. of physiological salt solution (precipitogen). One half (10c.c.) of this emulsion was injected into testicle and the other half into thoracic cavity of a rabbit. At an interval of 4 days, the injection was repeated in the same way, and after 15 days the serum was taken from the animal and tested upon precipitin, as shown in the following table, the results being read after one hour at room temperature (10-15°C.).
Precipitation experiments have been made in 4 cases of naturally affected cattle, 9 cases of artificially infected calves and 57 cases of controls. The results obtained are shown in the following table:
It must be noted here that in the precipitation experiment, all 4 cases of the affected cattle gave positive reaction in a dilution of from 1:640 to 1:1280 and all 9 cases of infected calves inoculated with the virus the same result in a dilution of from 1:40 to 1:1280, while cases of pericarditis (1), pleuro-pneumonia (1), tuberculosis (1), and influenza (1), as well as 53 cases of controls gave negative results.

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[in Japanese]

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(1) The coccidium was demonstrated in a stall-fed calf aet. one month old.
(2) In the earlier stages of the infection, the oocysts were demonsrated in the faeces, but not in the later stages.
(4) On autopsy a marked change were found in the colons as well as in the rectum, but no oocysts found in the contents of the intestines.
(4) The form of the oocysts is mostly ovoid, but some ones ellipsoid or spherical. The capsule has even thickness.
Among the ovoid ones, there are several irregular types, some being more ellipsoid, and other more spherical in their appearances. The size is 11.9-34.0μ in length, 10.2-25.5μ in breadth, but usually 25.5×18.7μ and 30.6×22.1μ. No mycropyle. No Restkörper even in the stage of the sporoblast and sporozoit.
(5) No growth at 37°C. 30°C. is the optimum temperarute, and it quit rapidly in this temperature. Slow development at 21-28°C., and the growth retards at 11-15°C. in the ice box.
(6) In giving the mature spores to a calf new oocysts will appear in the discharge within 5-12 days. It shows symptoms of the disease or remains intact, according to the number of the spores taken.

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[in Japanese]

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The hides estimated 10, 000, 000-11, 000, 000kin, and the animal hairs amounted to 600, 000-800, 000kin (with the exception of wool). were imported annually through the Osaka port for recent years.
Anthrax cases discovered in the examination of these samples are shown in the following table:
Since the year 1921 the importation of animal hairs to Japan, chiefly for the manufacture of brushes, became permissible after a course of disinfection at the Custom House at Osaka, Yokohama, and Tsuruga, but there is no ideal method for disinfection of imported hides as yet.

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1. The presence of the virus of contagious pleuro-pneumonia is constated in the circulating blood of the affected cattle, and it depends, as a rule, upon the nature of the disease.
2. The virus is present not only in the affected lung tissue and pleural exudate, but also in liver, spleen, kidney and lymph glands.
3. From above facts it will be safe to suppose that the blood may contain the virus and disseminate it through the body in a certain stage of the disease, and the circulating blood appears to be favourable in some extent for the propagation of the virus.

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