1. Of the above described 17 organs collected from the animals infected with rinderpest, the thymus and tonsil have been found to have the most superior antigenic property and the lung was proved to rank next to the spleen in the antigenic power.
2. It has been found that the minimum prophylactic dose of the lymphatic gland, thymus and tonsil is 0.15, 0.1 and 0.05 c.c. per kwan body weight respectively.
3. Inoculation experiments showed that the combined vaccines No. 506 (lymphatic gland and tonsil) and No. 493 (thymus and tonsil) were more effective than those made from any one of the the organs mentioned, and especially the vaccine No. 493 was proved to be highly efficacious.
4. Of various kinds of vaccines prepared by combining 2 or more of such organs, as spleen, lymphatic gland, thymus and tonsil, in various proportions, the one added with lung was found to be less efficacious than those lacking it, but was yet none of these vaccines the less efficacious as compared with those which have hitherto been made from the spleen.
5. It is not yet determined which is superior as vaccine, No. 497 containing the spleen, lung, lymphatic gland, thymus and tonsil, and No, 493 prepared only from the thymus and tonsil, as further experiments are required regarding the preservation of them.
It will be worthy of note that, by employing the combination method, the preparation of vaccine was highly improved, and the quantity of vaccine which can be prepared from one calf was amounted up to twice as much as before. For this reason we should like to designate the vaccine prepared by the combination method as "the economical rinderpest vaccine".

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I. The passage of the sheep-pox virus made through rabbit's testicles in successive inoculations of a piece of testicular tissues of each generation of which the first one having been inoculated intratesticularily with the skin eruptions, nodules of the lung, blood, lymphatic glands or bone-marrow obtained from an infected sheep may, upon the first or the third generation at the latest, present the typical lesions as the usual cow-pox virus does upon testicles of this animal. These lesions become more and more pronounced as the generations go on.
II. The testicular emulsion on the first generation remains always inert by the cutaneous inoculations upon calves, but the same after 2-4 passages at the latest, form the typical vesicles exactly same and indistinguishable from one produced by common cow-pox, which is seen more and more marked as the generations step on, fairly corresponding with the macroscopic changes in testicles.
III. The lymph prepared by the usual method from the vesicles produced on a calf with sheep-pox virus, which was passed through rabbit's testicles, may form on the skin of primary vaccinating infants the beautiful vesicles exactly similar to that of usual vaccine lymph.
IV. The immunity against common cow-pox virus rendered the rabbits, which were previously used for the sheep-pox passages, is found, about three weeks after the intratesticular inoculations, gradually becoming complete with the progress of generation, although no trace of immunity could be demonstrated in the first generation.
V. Powerful common cow-pox lymph effects absolutely inactive on a calf, which was inoculated with sheep-pox virus of 3-4 intratesticular passages in rabbits 16-20 days before, that is to say, the sheep-pox virus after passing through rabbit's testicles, differing from its original virus, maintain a complete immunizing property against cow-pox virus.
VI. The sheep-pox virus after intratesticular passages in rabbits considerably decreases its pathogenic character to sheep, as far as if with usual cow-pox virus.
VII. Sheep 19-20 days after having been inoculated with sheep-pox virus passed through rabbits intratesticularily are, by intravenous injection of the sheep-pox virus, typically affected with sheep-pox with exactly same incubation period as the untreated control, only remaining alive while the latter died of the disease after a week, and although it may still be beyond the determination as this minor number of the experimental animals would not stand for, that it is due to the fact of the incomplete immunization by the sheep-pox virus passed intratesticularily in rabbits, at least so far may be said with certainty, that the sheep-pox virus, after being passed through rabbit's testicles, renders the animal immune against the common cow-pox virus, losing, at the same time, the majority of its immunizing property against the original sheep-pox virus.
VIII. The sheep-pox virus is found transformed, as the above described experiments approve, perfectly into the cow-pox virus by passages through the testicular tissue of ascertaining that the small-pox and sheep-pox virus are, as being believed as such hithero, in very close relationship.

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I. Variola vaccine, when inoculated on the skin of sheep, produces vesicles, which take an intermediate form as they are found on the skin of calves and rabbits.
II. This form of vesicles is found on the sheep skin until 8th generation of successive transplanting, when the virulence seems suddenly impaired and in the 9th generation no vesicles can be prodnced, the only change on the skin being a desquamative inflammation of the smeared area. This continues until the 14th generation, when the virulence starts to recover itself again and reaches to its heights on the 23rd generation. After that no change was noticed until the last one (38th generation in the authors' experiment). For convenience sake the authors call the virus in these phases:
I stage of ovinization (1-8th generation)
II stage of ovinization (9-22nd generation)
III stage of ovinization (23rd generation and after)
III. Among these 54 sheep of the authors' expesiment not a single case of generalized reuption, severe general disturbances nor fatal case was encountered as they are described by Pissin as well as Fürstenberg. Just a remarkable raise in temperature was noticed in all animals.
IV. Linear inoculations of the original undiluted ovinized lymph on the calf skin produce typical vesicles up to the 8th generation (i. e. 1 stage of ovinization). The vesicles, however, become more and more atypically as the generations go on and after 11th transplantation no vesicles can be produced. This is also the case even with the lymph of the highest virulence (III stage of ovinization). That is to say, variola vaccine loses its virulence against calves with the complete adaptation to sheep body.
V. Inoculations on the shaven skin of rabbits with the 1:500 dilution of ovinized lymph of each generation until the 8th produce confluent or almost confluent vesicles. Further passage through sheep impairs the virulence of the lymph against rabbits, producing less or no vesicles but erythema on the rabbit skin, though the lymph attain the highest virulence against sheep. In another words, with the complete ovinization of variola vaccine by sheep passage the virulence of it against rabbits is almost lost.
VI. Ovinized lymph of the 20th generation was inoculated on two unvaccinated infants. Though ordinal variola vaccine, which was inoculated on the same individuals at the same time, produced typical vesicles no reaction was noticed with the ovinized lymph. This means that the ovinized lymph is avirulent also to human beings.
VII. In the early stages of sheep passage of variola vaccine it is immunogenic against the ordinal variola vaccine and never immuizing against inoculations with the ovinized veccine. With the progress of sheep passage, however, the immunizing property to the latter become more and more marked and the same to the former, on the contrary, decreases gradually, until it disappears altogether with the complete adaptation of the lymph to the sheep body, when the immunizing character of the lymph against the ovinized vaccine is perfectly developed.
VIII. Completely ovinized variola vaccine is highly immunizing against sheep-pox virus. But its practical application for the prevention of sheep-pox can not be relied upon, as it proved to be not potent enough for the immunization of animals.
IX. Sheep, which have recovered from sheep-pox infection, are not immune against ordinal variola vaccine. On the contrary, they are perfectly immune against ovinized variola vaccine, so that the latter seems very much more closely releted to sheep-pox virus in its characters.

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The results obtained from the experimants may be summarized as follows:
1. The veneral growth of the dog was transplanted to other dogs by intratesticular inoculation.
2. The transmitted growth represented a neoplasm rather than a connective tissue reaction.
3. No decision regarding the classification of the neoplasm was reached.

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Although the organism isolated from whale muscle sent in from Aomori prefecture is apparently identical with B. chauvoei and B. oedematis maligni, it can be definitely distinguished from these organisms biologically and serologically.
For differentiating the isolated organism from B. oedematiens and the bacillus of whale septicaemia (Christiansen), there were unfortunately no typical strains in our hand; so it is impossible to give any explanation regarding the relationships between the isolated organism and these anaerobes. But it should be placed into B. oedematiens group from the pathological lesions produced in the guinea-pig and a high pathogenicity for the rabbit.

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