The microsporidium, Pleistophora sp. NIS-M27, is a representative strain isolated from silkworms in rearing rooms, and Pleistophora sp. NIS-M27 along with Nosema bombycis NIS-001 and Vairimorpha sp. NIS-M11 is a standard strain for pébrine inspection. However, the molecular phylogenetic analysis of Pleistophora sp. NIS-M27 has not yet been performed. Thus, we performed a molecular phylogenetic analysis of Pleistophora sp. NIS-M27 (LC649920) based on the small subunit ribosomal RNA gene sequence. Additionally, we re-examined the relationship between small spore-producing microsporidian strains reported in previous studies and Pleistophora sp. NIS-M27. Phylogenetic analysis suggested the classification of Pleistophora sp. NIS-M27 into the genus Endoreticulatus. In particular, Pleistophora sp. NIS-M27 showed a high genetic homology of 99.83% with Endoreticulatus sp. BM-2018-2, and no other strain showed high genetic homology with the strain derived from the rearing room. This suggests that there are no strains genetically close to Pleistophora sp. NIS-M27 in the rearing room in recent years. From these results, we believe that Pleistophora sp. NIS-M27 should be classified in the genus Endoreticulatus. To accurately classify this strain in the future, multifaceted investigations, such as host infectivity and analyses based on other gene sequences, are required.
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