Amylase isozyme patterns of digestive juice were investigated using 103 silkworm strains. Three types of isozyme patterns were detected; that is, those possessing 4 or 5 cathodal bands (type-4 or type-5), or without any cathodal band (type-0). Fifty nine percent of 103 strains were grouped into type-0, then 29% and 12% of them were grouped into type-4 and type-5, respectively. The highest amylase activity was shown in type-5 strains followed by type-4, whereas amylase activity was weak in most of the type-0 strains. In the breeding strains, type-5 strains were small in number than type-0 strains. Isoelectric points of four isozymes found commonly in type-4 and type-5 strains were pI 8.95, 9.15, 9.35, and 9.50, and that of the rest isozyme found only in type-5 strains was pI 9.70. Furthermore, it was confirmed by means of two dimensional electrophoresis that each of 4 isozymes common in type-4 and type-5 was identical, and that amylase isozymes of midgut tissue were identical with those of digestive juice.
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