The Journal of Sericultural Science of Japan
Online ISSN : 1884-796X
Print ISSN : 0037-2455
ISSN-L : 0037-2455
Volume 58, Issue 4
Displaying 1-15 of 15 articles from this issue
  • SHIGEMI KAWASE
    1989 Volume 58 Issue 4 Pages 295-301
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
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  • YOSHIAKI SHIMIZU, MIHOKO DOHMYOU, TETSUO NAKAJIMA, MITSUO KIMURA
    1989 Volume 58 Issue 4 Pages 302-307
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    The dyeing behavior of Chinese tusser silk was examined and compared with that of domestic silk. The dyes used were acid-, disperse- and reactive disperse dyes. The dyeing rate of domestic silk yarn with C. I. Acid Orange 7 (OR II) was higher than that of Chinese tusser silk yarn up to 900 seconds. However, elapsing the time reversed the situation after that. The diffusion coefficients of OR II in silk samples were obtained by Hill's approximate equation. The diffusion coefficient of OR II in Chinese tusser silk yarn was larger than that in domestic silk yarn. The thermodynamic parameters were calculated from the data of the dyeing of silk samples with OR II at equilibrium state. The binding constant, standard affinity and the heat of dyeing of OR II for domestic silk were larger than those in case of Chinese tusser silk. This difference results from the difference in amino acid compositions of these silks, namely the sort and amount of functional group interacting with OR II are different each other. Adsorption of disperse dye on Chinese tusser silk was the exothermic reaction, the heat of dyeing was smaller than that in case of domestic silk. Also, standard affinity of disperse dye for Chinese tusser silk was smaller compared to domestic silk. The more amounts of a reactive disperse dye of sulfatoethylsulfonyl type for domestic silk were exhausted and fixed in the lower dye concentration, but the situation reversed in the higher dye concentration, that is, the dye preferred Chinese tusser silk.
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  • TOSHIHIKO IIZUKA, NAOKO TERAE
    1989 Volume 58 Issue 4 Pages 308-314
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    Insecticidal crystal proteins producd by the typical strains of Bacillus thuringiensis (subsp. thuringiensis berliner, subsp, kurstaki HD-1, subsp. kurstaki HD-73, subsp. sotto, subsp. dendrolimus, subsp. aizawai IPL, subsp. aizawai juroi, subsp. darmstadaensis, subsp, israelensis and subsp. kyushuensis) were analyzed by SDS-PAGE and V8 protease-PAGE. Molecular weights of protoxins resulted from SDS-dissolution of the parasporal inclusions or SDS-dissolution followed by V8 protease digestion, were compared. The peptide maps of the protoxins demonstrated by the present study, could be one of the most reliable and useful characters for the identification of B. thuringiensis strains.
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  • JUN SHIMADA
    1989 Volume 58 Issue 4 Pages 315-321
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    Activity of prothoracicotropic hormone (PTTH) in corpora allata and brain, and timing of PTTH release were examined by surgical operation using 5th instar female larvae of Bombyx mori L. (J106×Daizo). These larvae stopped feeding about 120hr, purged the gut contents (so called “gut purge”) about 130hr and pupated about 190hr after the time of first feeding folloing the 4th molt. From the implantation experiments into neck-ligated 4th instar larvae, it was found that PTTH activity exists in corpora allata and brain throughout the 5th instar except for the brain of larvae immediately after ecdysis. Effects of extirpation of corpora allata, brain or both organs from the 5th instar female larvae on the timing of the gut purge and pupation were investigated intermittently during the 5th instar. From results of these experiments, it was suggested that PTTH was released in twice during last larval instar. The first release was assumedly within 96-120hr and the second release was assumedly within 120-144hr after the time of the first feeding in the 5th instar, respectively. However, it seemed likely that PTTH had leaked out into hemolymph before the first release and there was a considerable difference among larvae in the grade of its leakage.
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  • HIDEYA ASAKAWA, KUNIKATSU HAMANO
    1989 Volume 58 Issue 4 Pages 322-326
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    Amylase isozyme patterns of digestive juice were investigated using 103 silkworm strains. Three types of isozyme patterns were detected; that is, those possessing 4 or 5 cathodal bands (type-4 or type-5), or without any cathodal band (type-0). Fifty nine percent of 103 strains were grouped into type-0, then 29% and 12% of them were grouped into type-4 and type-5, respectively. The highest amylase activity was shown in type-5 strains followed by type-4, whereas amylase activity was weak in most of the type-0 strains. In the breeding strains, type-5 strains were small in number than type-0 strains. Isoelectric points of four isozymes found commonly in type-4 and type-5 strains were pI 8.95, 9.15, 9.35, and 9.50, and that of the rest isozyme found only in type-5 strains was pI 9.70. Furthermore, it was confirmed by means of two dimensional electrophoresis that each of 4 isozymes common in type-4 and type-5 was identical, and that amylase isozymes of midgut tissue were identical with those of digestive juice.
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  • MASUHIRO TSUKADA, MASANOBU NAGURA, HIROSHI ISHIKAWA
    1989 Volume 58 Issue 4 Pages 327-331
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    The effect of the moisture absorption on the fine structural changes of silk fibroin fiber was carefully examined by thermomechanical analysis, X-ray diffractometry and on the basis of the results of refractive index and strength-elongation measurements. Increase of the moisture absorption caused a decrease of birefringence of the silk fiber. Rupture strength of the sample fiber decreased gradually, while the elongation at break clearly decreased when the amount of moisture absorption increased. The amount of the contraction appeared on the thermomechanical curve of silk fiber in the temperature range 130-190°C increased gradually with increasing the amount of the moisture absorption. However, the molecular orientation estimated from the X-ray diffraction curve corresponding to the molecular orientation of the crystalline region stayed unchanged regardless of the moisture absorption. These findings suggest that the decrease of the molecular orientation of the silk fibroin fiber is greatly attributed to the decrease of the molecular orientation in the amorphous region but not in the crystalline region.
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  • MINORU HAMAZAKI
    1989 Volume 58 Issue 4 Pages 332-337
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
  • YUTAKA KAWAGUCHI, HIROSHI DOIRA, YUTAKA BANNO, HIROSHI FUJII
    1989 Volume 58 Issue 4 Pages 338-343
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    The influence of N-methyl-N-nitrosourea (MNU) treatment on morphogenesis of Bombyx mori was investigated. Eggs of an inbred normal strain of B. mori at 0.5 to 24hr after the laying were immersed in aqueous solution of MNU for 15min. MNU-immersion of eggs resulted in various anomalies on hatched larvae such as segment fusion, twist of the body axis, deletion and/or surplus of the legs and so on. Frequency of the malformed larvae was extremely high when treated at 1.5 to 2.5hr egg age undergoing the syngamy of egg and sperm nuclei. It was also recognized that the incidence of the monstrosities was high in the middle parts of abdominal region from the sixth to the ninth body-segment throughout irrespective to the egg age at the onset of MNU-treatment. Monstrosity frequencies arrising in the anterior parts of body including head and thorax and those in the posterior parts were rather low being dependent on the egg age at the MNU-treatment.
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  • MASARU KOBAYASHI, SHIGEO YANAGAWA, KAZUYUKI TANAKA
    1989 Volume 58 Issue 4 Pages 344-348
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    It has been found that repeated outdoor rearing of wild silkworms at a single farm causes contamination of the farm with nuclear polyhedrosis viruses (NPV), which results in unstable production. Therefore, a synthetic evaluation was performed on the cause of the disease and methods for providing stable production, such as the possibility of transmitting the viruses by insects inhabiting the farm and a method to inactivate the pathogen in soil and rearing trees. The results obtained are as follows. 1) Ants and aphides, high-density inhabitants of the farm, aggravated the transmission of the disease's viruses. 2) These viruses were found remaining on the branches and leaves of the red oak trees after rearing was completed. 3) Most of remaining viruses in soil were found to be accumulated in the surface layer to a depth of about 2cm. 4) The remaining viruses in soil could be inactivated using a flamethrowing sterilization device (tip temperature, 1, 800°C). 5) The decrease in the silkworm production during the young stages was mostly due to destruction by ants.
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  • HIROAKI MACHII
    1989 Volume 58 Issue 4 Pages 349-350
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
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  • HAJIME FUGO
    1989 Volume 58 Issue 4 Pages 351-352
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
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  • TOSHIO OHNISHI, KOJI TANABE
    1989 Volume 58 Issue 4 Pages 353-354
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
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  • KUNIKATSU HAMANO, KOZO TSUCHIDA
    1989 Volume 58 Issue 4 Pages 355-356
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
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  • YUKIO YANAGISAWA, HIDENARI SHIOIRI
    1989 Volume 58 Issue 4 Pages 357-358
    Published: August 27, 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
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  • 1989 Volume 58 Issue 4 Pages 360
    Published: 1989
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
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