The Journal of Sericultural Science of Japan
Online ISSN : 1884-796X
Print ISSN : 0037-2455
ISSN-L : 0037-2455
Volume 69, Issue 6
Displaying 1-6 of 6 articles from this issue
  • MASAYUKI NOZUE, WEI CAI, LING LI, WENXIN XU, HIDENARI SHIOIRI, MINED K ...
    2000 Volume 69 Issue 6 Pages 345-352
    Published: December 29, 2000
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    A reliable method for Agrobacterium-mediated transformation of mulberry (Mores alba L.) callus has been developed. Calli initiated from cotyledons, hypocotyls and roots of seedlings were co-cultivated with Agrobacteria in the presence of acetosyringone under acidic pH (pH5.5) at low temperature (20-22°C). Putative transgenic calli were selected by using a medium containing 12.5μg/ml hygromycin. When the calli initiated from hypocotyls and A. tumefaciens strain EHA101/pIG121-Hm were used, the highest transformation frequency, as evaluated by resistance to hygromycin, was obtained. Completely transformed calli were obtained through several subculturings by gradually increasing the concentration of hygromycin in the selection medium. GUS assay and PCR analysis clearly indicated that the obtained hygromycin-resistant calli were transformed with T-DNA from binary vector to mulberry genome.
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  • FUMIAKI TAJIMA, YAPING ZHOU, YOSHIO ISHIGURO
    2000 Volume 69 Issue 6 Pages 353-358
    Published: December 29, 2000
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    Silk yarn is biodegraded by the microorganism which adheres to it and multiplies in the soil, when it is embedded in soil. It is important to estimate the biodegradability of silk yarn from the point of view of its application to earthwork. In order to improve the precision of estimating the biodegradability with weight loss of silk yarn, we applied the picture processing method to presume the degree of biodegradation. The experimental result of the degree of biodegradation with picture processing was greater than that of the weight loss in the case of silk yarn embedded in soil for two months. This result suggested that it was more effective in grasping the degree of biodegradation with picture processing than that with weight loss.
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  • TOMOAKI OKI, MIYOKO TSUKAHARA, HISAYA WATASE, HARUO KINOSHITA
    2000 Volume 69 Issue 6 Pages 359-362
    Published: December 29, 2000
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    When a figured fabric was woven on the jacquard loom in our institute, the weaving bars appeared. In order to clear up the cause of appearance of the weaving bars, the following items of the weft yarns were investigated on the defective woven fabrics; (1) pick spacing, (2) number of twist, (3) size of denier, (4) degumming loss percentage, (5) dye absorption percentage. The results obtained were as follows; (1) the weaving bars appeared in the part of excess twist of weft yarns, (2) the size of denier of weft yarns had not relation to the weaving bars, (3) the degumming loss percentage of weft yarns had some bearing with the weaving bars, and (4) the number of twist of weft yarns was linked with dye absorp-tion percentage. These results indicated that the weaving bars were caused by decreasing dye absorption percentage due to twist of the weft yarns.
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  • KAZUKI YAMAZAKI, TARO USUDA, AKIO SAKAGUCHI, KOICHIRO TORIUMI
    2000 Volume 69 Issue 6 Pages 363-368
    Published: December 29, 2000
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    The mechanical characteristics and the hand performances of black silk yarns dyed via vegetable color dyeing were discussed. The silk yarns were dyed with six black dyes extracted from different plants according to Japanese traditional dyeing method. The hand performances of six silk yarns were evaluated using the paired comparison method and were compared with the measured mechanical properties. The hand performances and mechanical properties of the yarn were influenced by the dyestuff used, and the changes in hand performances and the bending characteristics showed the similar tendency when the dyeing procedure was repeated.
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  • EIICHI KOSEGAWA, G. VEMANANDA REDDY, KUNIMITU SHIMIZU, TERUO OKAJIMA
    2000 Volume 69 Issue 6 Pages 369-375
    Published: December 29, 2000
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
    Silkworm races are experientially classified into three types of voltinism, such as univoltine, bivoltine and multivoltine, though the voltinism tends to be influenced by emviron-mental condition at various stages. We investigated the induction of non-diapause eggs by dark and low temperature incubation (constant darkness, 15°C) in the local varieties preserved in the National Institute of Sericultural and Entomological Science as genetic resources. Fourty two of 87 univoltine races tested produced only diapause eggs, while five races produced non-diapause egg batches only. Bivoltine races produced non-diapause eggs except one (Seihaku-Ymagata). The character producing non-diapause eggs seemed to be maintained appropriately. While incidence of non-diapause eggs was remarkably high in European races, there was no big difference between Japanese and Chinese races. Two races of the Koishimaru (Sanshi and Watari) were distinguishable from each other by the incidence of non-diapause eggs, even though it was difficult to distinguish them by the external appearance. These data on voltinism of the local silkworm varieties will be recorded into the data base of our Institute and opened to the public in the near future.
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  • YUTAKA KAWAHARA, HIDETOSHI TAMURA
    2000 Volume 69 Issue 6 Pages 377-379
    Published: December 29, 2000
    Released on J-STAGE: July 01, 2010
    JOURNAL FREE ACCESS
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