All issues
Volume 70 (2001)
- Issue 3 Pages 135-
- Issue 2 Pages 85-
- Issue 1 Pages 1-
Predecessor
Volume 70, Issue 1
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
HARUNORI KIKUTA, NORIKO IGARASHI, CHIKA TATEBAYASHI, SACHIKO OGINO, KE ...2001 Volume 70 Issue 1 Pages 1-9
Published: April 27, 2001
Released on J-STAGE: July 01, 2010
JOURNAL FREE ACCESSSpecimens from wild strains of Bacillus thuringiensis were collected from 200 partitioned survey sites in each of the following two places and were examined for the morphology of parasporal inclusions (PI) under a scanning electron microscope and for the H antigen distribution by an immunological method. The results are summarized as follows. 1) In an area in the Nohoro Forest Park, a representative lowland of the Hokkaido forest, 39 strains of B. thuringiensis were isolated from 17 (8.5%) of the 200 sampling sites. At 14 of the 17 sites, the density was as low as one strain per sampling site. The isolated strains were comprised of four H antigen types: 5a5b, 16, 4a4c and 14. H antigen type 5a5b was dominant, occupying about 61% of the total strains. All strains were one of five types in terms of parasporal inclusion morphology: bipyramidal & cuboidal, bipyramidal & cuboidal & irregular, irregular and cuboidal. The strain named IL1-7-2 was incapable of producing bipyramidal parasporal inclusions, but it produces a remarkably large quantity of cuboidal parasporal inclusions and was considered to be of a new type different from the type serovars. 2) In an open-air chapel of Rakuno Gakuen University, wherein trees are well conserved, 70 strains of B. thuringiensis were isolated from 26 (13%) of the 200 survey sites. At 24 of the 26 sites, the density was as low as four or fewer strains per area. The isolated strains were comprised of 11 H antigen types: 1, 3a3b, 4a4b, 5a5b, 6, 7, 8a8b, 10, 14, 16 and 24a24b. Again the H antigen type 5a5b was dominant, occupying about 60% of the total strains. As to the parasporal inclusion morphology, all strains were one of seven types, including collapsed bipyramidal and collapsed cuboidal. The strains named MR10-3-3, MR10-5-1, MRIO-5-2, MRIO-5-3, MR10-6-2 and ML4-2-1 were incapable of producing bipyramidal parasporal inclusion, but only produce cuboidal parasporal inclusion and were thus considered to be of a new type different from the type serovar.View full abstractDownload PDF (1097K) -
TAKAHIKO NISHIOKA, KEN NAKAZAWA, KAR WAI CHOW2001 Volume 70 Issue 1 Pages 11-15
Published: April 27, 2001
Released on J-STAGE: July 01, 2010
JOURNAL FREE ACCESSThe Akaike Information Criterion (AIC) can be used to obtain the best fitting order of the fundamental cocoon shape. Principal Component Analysis as mentioned in the previous paper can represent the difference of the shape of the cocoon. However, it was not the absolute shape of the cocoon represented in the mathematical sense. Then, the AIC was applied to obtain the order of regression coefficients of the shape of the cocoon waveform. As a result, it was two, three or four terms needed to attain the minimum AIC for every sample cocoon in Chinese races. Japanese races minimized AIC when three or four terms were used, whereas it was minimized when three terms were used for the hybrids. In the cases of obtaining the regression coefficients for each race, three terms were the best number of terms in almost races though one Japanese race needed four terms.View full abstractDownload PDF (1737K) -
LUN BAI, JIANMIN WANG, ZHAO ZHOU2001 Volume 70 Issue 1 Pages 17-22
Published: April 27, 2001
Released on J-STAGE: July 01, 2010
JOURNAL FREE ACCESS -
LUN BAT, JIANMIN WANG, ZHAO ZHOU2001 Volume 70 Issue 1 Pages 23-29
Published: April 27, 2001
Released on J-STAGE: July 01, 2010
JOURNAL FREE ACCESS -
KUNIAKI FUKUI2001 Volume 70 Issue 1 Pages 31-36
Published: April 27, 2001
Released on J-STAGE: July 01, 2010
JOURNAL FREE ACCESSThe prediction of mulberry (Morus alba L.) growth becomes important for changes in the mulberry cultivation system. To develop a mulberry growth model, the response of mulberry growth to different temperatures was investigated. Mulberry saplings were grown in phytotorons under sunlight at 3 diurnally fluctuating temperature conditions (34/22, 32/24, 30/26°C) which were a 28°C average daily temperature and at 2 constant temperatures (28, 32°C) in 1995, and at 4 fluctuating temperatures (33/19, 32/20, 30/22, 28/24°C) which were a 26°C average daily temperature and at one constant temperature (26°C) in 1996. The shoot length and leaf number were investigated every 10 days. Distribution of dry matter to each plant part was measured at the end of the treatment. Changes in shoot length were not different among temperature treatments and the rate of shoot elongation increased in the process of time during the treatments in 1995, but leveled off at the end of treatment in 1996. There were no differences in leaf number among the same average daily temperature treatments in either years, but the leaf number between 32°C treatment and the other average daily 28°C treatments in 1995 was significantly different at a 1% level. Shoot dry weight was not different among temperature treatments in both years. Therefore, fluctuating temperature conditions seemed not to affect mulberry growth as long as the average daily temperature was same. The leaf number seemed to be a superior index of mulberry growth.View full abstractDownload PDF (730K) -
TAKAKO MIYAJIMA, TOSHIO YAMAMOTO, KEISUKE MASE, TETSUYA IIZUKA, MINORU ...2001 Volume 70 Issue 1 Pages 37-42
Published: April 27, 2001
Released on J-STAGE: July 01, 2010
JOURNAL FREE ACCESSIn order to produce superthin cocoon filaments, trimolter larvae were induced from two tetramolter races, Hakugin and Honobono, by the imidazole compound, triflumizole, (E)-4-chloro-α, α, α-trifluoro-N-[1(1H-imidazole-1yl)-2-propoxyethylidene]-o-toluidine. When an artificial diet containing 140ppm of triflumizole in dry weight was fed to 3rd or 4th instar larvae for 3 days after ecdysis, more than 80% of the larvae eliminated the 4th instar in Hakugin but less than 30% did in Honobono. The higher concentration (280ppm) increased the percentage of trimolter up to nearly 100%. It was found that the low susceptibility to triflumizole in the hybrid “Honobono” (J513×C514) was derived from the parental strain, J-513. Percentages of trimolter induced by the treatment in the 3rd instar was higher than that in the 4th instar treatment. Larval duration in the 4th instar treatment was shorter than that in the 3rd instar treatment. Effect of triflumizole on the quantitative characters of the cocoon was higher in turn as follows, cocoon shell weight>cocoon weight>cocoon filament size>cocoon filament length>cocoon shell percentage>cocoon length>cocoon width. Since the decreasing rate of cocoon length is greater than that of cocoon width, the cocoon form of trimolter became rounder compared to the untreated tetramolter control. Cocoon filament sizes of the trimolters induced by the 3rd instar treatment in Hakugin and Honobono were 1.36 and 1.72 denier, respectively, and those induced by the 4th instar treatment were 0.99 and 1.03 denier, respectively. Furthermore, the deviation of a filament size in cocoon of these trimolter was remarkably small. We believe these superthin cocoon filaments will be very useful as new silk materials.View full abstractDownload PDF (751K) -
YUJI KAWAKAMI, HIDETOSHI IWANO, YOSHINORI HATAKEYAMA, TADASHI INOUE, E ...2001 Volume 70 Issue 1 Pages 43-48
Published: April 27, 2001
Released on J-STAGE: July 01, 2010
JOURNAL FREE ACCESSThe specific amplification by PCR using a pair of primers, KAI01 and KAI02 (KAWAKAMI et al., 1995) was reinvestigated as to its ability to distinguish microsporidia belonging to genus Vairimorpha, Pleistophora and Nosema. DNA samples from microsporidia of genus Vairimorpha or Pleistophora so far tested, three species of Vairimorpha and one Pleistophora, failed to produce PCR products by those primers. Of Nosema spp., N. apis, N. furnacalis, N. mylittensis and Nosema tyriae from cinnabar moth, Tyria jacobeae did not also produce any product. Microsporidian parasites isolated from silkworms infected with pebrine in the northern part of India (courtesy of Dr. A. K. Bansal) and in southern India (courtesy of Dr. KIYOSHI KAWAKAMI) and in China (courtesy of Dr. X. CHEN) gave PCR products by KAI01 and KAI02 primers. The results from our study suggest that PCR tests with the use of KAI01 and KAI02 primers are useful to distinguish highly virulent strains of N. bombycis in pebrine inspection.View full abstractDownload PDF (4409K) -
TAKESHI KAKO2001 Volume 70 Issue 1 Pages 49-53
Published: April 27, 2001
Released on J-STAGE: July 01, 2010
JOURNAL FREE ACCESSDownload PDF (508K)
- |<
- <
- 1
- >
- >|