The present paper deals with the change of the voltinic characteristics due to the injection of a few inorganic salts and some inhibitors for enzymes using two kinds pupae induced respectively by the low temperature incubation (15°C dark) and the high temperature one (25-26°C light) in the embryonal stages using four chinese bivoltine races, the former usually will lay the non-hibernating eggs, but the latter do the hibernating ones.
In the experiments of the injection used the pupae induced by the low temperature incubation of the eggs, some pigmented and hibernating eggs were obtained by the injection of KCl and Na-citrate. In the NaCl injection, a few lightly pigmented eggs were obtained, being all of them non-hybernating eggs. In the investigation on the effects of a few inhibitor for some enzymes, it was observed that Na-azide which is thought to inhibit the cytochrome oxidase activity has some influences upon the change of the egg pigmentation and hibernation, and other inhibitors for enzymes produced very lightly pigmented eggs, but they were all non-hibernating ones.
The phenotype of the eggs from the female moth being injected KCl, NaCl and K
2SO
4 in the pupal stage were resembled to the mutant kidney (
ki), end this may be thought to be a kind of phenocopy.
In the experiments using the pupae treated in the embryonal stage with high temperature, the eggs not showing pigmentation and hibernation of several percentage were induced in almost all cases of the injection of some inorganic salts and a few inhibitors for some enzymes.
In the experiments on the injection of the above mentioned substances into the larval body, no appearent influences upon the change of hibernation were observed, though in a few cases lightly pigmented eggs appeared.
The changes of the voltinic manifestation by the above mentioned injection may not be due to the direct effects on the function of the hormon which determine voltinism and that of the suboesophagial ganglia or brain, but may be due to the effects on the egg cells in the special developmental stage.
The mechanism on the change of the voltinism induced by the injection of KCl and NaCl are thought to be different from the effects of the inhibitor for enzymes, and it may be plausibly thought that K plays an important part for the change from the non-hibernating eggs to the hibernating ones.
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