Grape pomace is the solid waste material (skin, stem, leaf, and seed) that remains after pressing juice from the grapes. At present, it is mainly used as animal feed or for composting. This study has identified some of the useful
components of grape pomace with a view to more effective utilization of this material in the future. We quantified the polyphenol concentrations of methanolic extracts of grape pomace from different varieties of grape. For each grape pomace, we used liquid chromatography-mass spectrometry (LC-MS) to detect the presence of anthocyanins and liquid chromatography -tandem mass spectrometry (LC-MS/MS) was used to assign each chromatogram peak. The effects of these components on the differentiation of 3T3-L1 cells were assessed. Polyphenols were found to be effectively retained in the grape skin (campbell, niagara, kerner, and chardonnay) in the grape pomace. Campbell pomace skin, merlot dried pomace leaf, kyoho pomace seed, and chardonnay pomace stem were analyzed, and anthocyanidins with sugars or organic acids were detected in the skin and leaf samples. Delphinidin detected in the skins was observed to inhibit the differentiation of 3T3-L1 cells.
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