In order to establish a routine procedure for the quality control of mass-reared Dacus cucurbitae COQUILLETT, six acoustic properties of tethered flight sounds of the wild (W-), mass-reared nonirradiated (M-), and
60Co-irradiated (I-) strains of the male flies were examined in eight age groups. By multivariate analyses we determined that the most effective factors for discriminating between strains were; parameters of fundamental frequency, peak power density of the fundamental frequency, total harmonic distortion, and the bandwidth of the fundamental frequency at ages of 5, 13, 9, and 22 days. Mahalanobis' D
2 obtained on six acoustic parameter variables in a linear discriminant analysis showed that the overall relative differentiatin between W- and M-strains was 1.3, and that between W- and I- strains was 1.4, times as large as it was between M- and I-strains. Histographical records produced by this discriminant analysis will be useful in monitoring the laboratory-reared strain for timely warning of qualitative changes before release.
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