巻号一覧
25 巻 (2004)
- 6 号 p. 249-
- 5 号 p. 209-
- 4 号 p. 165-
- 3 号 p. 109-
- 2 号 p. 61-
- 1 号 p. 1-
25 巻, 4 号
August
選択された号の論文の6件中1～6を表示しています
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Full Papers
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Kenichi MATSUZAKA, Kazumasa OHTA, Ken TAKAHASHI, Nahoko MIYAKE, Masayu ...2004 年 25 巻 4 号 p. 165-170
発行日: 2004年
公開日: 2005/07/22
ジャーナル フリーDifferent types of osteoblast behavior result from external stimulation in the early and in the late stages of cell differentiation. The purpose of this study was to investigate the influence of a diode laser on osteoblasts and to compare any effects between the proliferating and the differentiating phases. In the proliferating phases, the numbers of cells greatly increased in the laser irradiated group as compared with that of the control group. The increases in the numbers of cells in the differentiating phase were no greater than were those in the proliferating phase. The alkaline phosphatase activity of cells in the proliferating phase was highest 5 days after the laser irradiation, however, the activity in the differentiating phase increased day by day. Although osteopontin mRNA levels in the proliferating phase increased in the first 3 days of culture and then decreased in both groups, this decrease of osteopontin mRNA was small in the irradiated group. In contrast, osteopontin mRNA expression in the differentiating phase increased at day 1 and then decreased in the control group, while osteopontin mRNA levels in the irradiated group did not decrease.抄録全体を表示PDF形式でダウンロード (2145K) -
relationship with renal failureMasahito TSUCHIDA, Toru NAGURA, XeuFeng BAI, ChangChun LI, Chikako TOM ...2004 年 25 巻 4 号 p. 171-178
発行日: 2004年
公開日: 2005/07/22
ジャーナル フリーMice were fed for 3 days without drinking water to produce dehydration. Lymphocytes and leukocytes yielded by the liver, spleen, thymus, kidney, and bone marrow decreased gradually from day 1 to day 3. The bone marrow which contained mainly granulocytes was most resistant to the abovementioned cytopenia. The remaining lymphocytes on day 3, namely dehydration-resistant cells, were found to be extrathymic T cells (i.e., IL-2Rβ+CD3int cells or NKT cells) in all tested organs. Interestingly, granulocytes (i.e., Mac-1+Gr-1+) appeared on day 3 in various organs, including the kidney. Dehydration induced an elevation of hematocrit in the blood and showed evidence of renal and hepatic failure. Granulocytes isolated from these organs were at activated states in terms of superoxide production. Serum levels of corticosterone and plasma levels of catecholamines were found to increase due to dehydration. These results suggest that dehydration is intimately associated with immunosuppression and reciprocal granulocyte activation, which may finally induces renal failure by activated granulocytes.抄録全体を表示PDF形式でダウンロード (2900K) -
Shinji SUGIMOTO, Mikio OTA, Yoshihiro SHIBUKAWA, Satoru YAMADA2004 年 25 巻 4 号 p. 179-187
発行日: 2004年
公開日: 2005/07/22
ジャーナル フリーThe present study evaluated whether transplanted proliferating tissue in periodontal osseous defects can form new periodontal ligament. A periodontal osseous defect was created in dogs by removing alveolar bone around the circumference of the premolar, and was covered with the membrane or without the membrane at control sites. After two weeks, the proliferating tissue in the periodontal osseous defect under the barrier membrane was excised, while at each control site, the connective tissue was dissected. The proliferating tissue and tooth were transplanted to holes drilled in the alveolar bone, while at the control sites, the connective tissue and tooth were transplanted. After one, two and four weeks, the dogs were sacrificed and examined histologically; a LightCycler was also used to measure the mRNA expression of vascular endothelial growth factor (VEGF) and alkaline phosphatase (ALP) in the transplanted tissues. VEGF and ALP mRNA expressions were significantly increased in the transplanted proliferating tissue compared with the connective tissue (control). The root transplanted bearing the proliferating tissue formed new periodontal ligaments, whereas connective tissue could be observed on the root surface in the controls. The results of this study show that transplanted proliferating tissue promotes the formation of new periodontal ligaments around the root.抄録全体を表示PDF形式でダウンロード (3527K) -
Ufuk ÇAKATAY, Refik KAYALI, Serpil SALMAN, Ahmet SIVAS, Ilhan S ...2004 年 25 巻 4 号 p. 189-193
発行日: 2004年
公開日: 2005/07/22
ジャーナル フリーWe studied 51 early stage type 1 diabetic patients and 48 healthy normolipidemic age-matched controls to examine the influence of lipid profile on lipid hydroperoxide levels. The type 1 diabetic patient group consisted of 12 prepubertal children, 18 adolescents, and 21 young adults who were free from any clinical evidence of retinopathy, nephropathy, or neuropathy. We determined serum triglyceride, total cholesterol, HDL (high-density lipoprotein)-cholesterol, LDL (low-density lipoprotein)-cholesterol, VLDL (very low-density lipoprotein)-cholesterol, apolipoprotein A-1, apolipoprotein B, lipoprotein (a) levels as markers of lipid profile, and lipid hydroperoxide levels (LHP) as markers of lipid peroxidation, and tried to show the relation, if any, between lipid profile and lipid peroxidation. Serum lipid profiles of three groups of diabetic patients were not different compared to their controls. However, LHP levels were increased significantly in adolescent and young adult type 1 diabetic patients compared to their controls. The significant increase in LHP levels in these two groups of patients was independent from their lipid profile parameters except triglycerides. The lack of difference in LHP levels in prepubertal diabetic patients and their controls might be explained by intense hormonal and metabolic changes during puberty.抄録全体を表示PDF形式でダウンロード (2705K) -
Akihiro KONNO, Yoshiaki OOTA, Yoshiharu HASHIMOTO, Yasuhiro KON, Toshi ...2004 年 25 巻 4 号 p. 195-200
発行日: 2004年
公開日: 2005/07/22
ジャーナル フリーThe non-essential amino acid L-serine is biosynthesized from 3-phosphoglycerate by astrocytes, and functions as a key mediator of neurotrophic support in the brain. In the present study, the cellular localization of 3-phosphoglycerate dehydrogenase (3PGDH), a key enzyme in L-serine production, in tissue outside the central nervous system of the mouse was examined by immunohistochemistry and in situ hybridization. In the peripheral nervous system of adult mice, 3PGDH immunoreactivity was found in satellite cells of the dorsal root ganglia and intestinal nerve plexuses. Endocrine tissues contained 3PGDH-immunoreactive supporting cells, such as stellate cells of the adenohypophysis and sustentacular cells of the adrenal medulla. In genital tissues, Sertoli cells and spermatogonia of the testis and oocytes in the primary ovarian follicle were immunolabeled with the 3PGDH antibody. The lens epithelium and retinal pigment epithelium were most intensely immunoreactive among sensory organs. The expression of 3PGDH mRNA in the eye and testis was detected by in situ hybridization in cells corresponding to those in which it was detected by immunohistochemistry. Moreover, 3PGDH immunoreactivity was confirmed in the lens placode and in both the Sertoli and primitive germ cells of embryos, all of which are tissues affected by congenital diseases in human 3PGDH deficiency.抄録全体を表示PDF形式でダウンロード (2461K) -
Kazuto EBE, Chikako TOMIYAMA-MIYAJI, Hisashi YOKOYAMA, Yuiko ISHIMOTO, ...2004 年 25 巻 4 号 p. 201-207
発行日: 2004年
公開日: 2005/07/22
ジャーナル フリーThis study focused on double-positive (DP) CD4+8+ cells in both the intestine and thymus. In normal mice, the proportion of DP cells in the intestine increased with aging, while that of DP cells in the thymus remained unchanged. When mice were exposed to restraint stress for 18 h, intestinal DP cells were resistant to restraint stress, while thymic DP cells were sensitive to such stress in terms of apoptotic properties. Intestinal intraepithelial lymphocytes (IEL) and thymocytes were then cultured and the number of living cells were analyzed. The number of DP cells in the thymus decreased prominently in in vitro culture. Interestingly, a similar phenomenon was found in intestinal DP cells in in vitro culture. When thymocytes were isolated from mice exposed to restraint stress, these DP cells were found to become sensitive to apoptosis in in vitro culture. Phenotypic characterization revealed that DP cells in the intestine were CD3+CD25−CD44+CD69+, while those in the thymus were CD3−CD25+CD44−CD69−. These results suggest that DP cells in the intestine were more mature than those in the thymus and that DP cells of IEL and thymus were generated as primitive T cells in phylogeny but later developed along independent pathways at each site.抄録全体を表示PDF形式でダウンロード (2416K)
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