Experiments were conducted to determine the difference in microstructure between Nagoya-Cochin (NC) and White Leghorn (WL) eggs.
In order to characterize the microstructure of the NC yolk, the size and shape of the yolk sphere were measured by using image processing techniques and compared to those of WL eggs. Two kind of egg were used: 1) 15 NC eggs, and 2) 6 MS-sized WL eggs from chickens receiving the same diets. Each egg was steamed for 15 min, and the yolk was removed and weighed. The yolk was divided into the outer, middle, and inner layers, which were each immersed in the fixation solution. The yolk samples were dehydrated by graded ethanol, and yolk spheres immersed in 100% ethanol were observed with an image processor. These parameters were measured for about 150 yolk spheres per sample: the transverse sectional area, the ratio of long to short dimensions, and maximum dimension. Other yolk samples were also observed by scanning electron microscopy.
The results reveal that the weights of NC eggs and NC yolks was not significantly different from those of WL eggs. The average transverse sectional area of the middle layer of the NC yolk spheres was 4502μm
2, and of the WL yolk spheres was 7027μm
2. The NC yolk spheres were thus significantly smaller than the WL yolk spheres, while the ratio of long to short dimension of the NC yolk spheres was also smaller than that of the WL yolk spheres.
The size of the yolk spheres thus varies according to hen strain.
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