The aim of this study was to investigate the caseinolytic activity of the homemade shio-koji prepared from dry rice-koji. The results obtained were as follows:
1)The caseinolytic activity of the shio-koji decreased 84% after fermentation and maturation for 7 days at 24°C; however, after 2 months of refrigeration, the same level of activity was maintained. The sugar content of shio-koji increased slowly after it increased to 20% from 10% for the first 7 days. The salt concentration of the shio-koji was maintained at about 11% during the entire storage period.
2)Casein degradation by a 1:10 diluted solution of shio-koji that was used for the activity measurement progressed in a time dependent manner for at least 6 hours. Casein degradation was also assessed by SDS-PAGE.
3)The caseinolytic activity of undiluted shio-koji did not change after 30 minutes at 50°C, but its activity declined at higher temperatures.
4)The caseinolytic activity of shio-koji was three times that of ginger and about 1/8 of the activity exhibited by kiwi-fruit.
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