The cytologic localization and cellular levels of
myc oncoprotein in the human ovary during follicular growth, regression and atresia were examined by the avidin/biotin immunoperoxidase method with a specific antibody to
myc oncoprotein. In primordial follicles, only the oocyte showed intense immunostaining for
myc protein, whereas the granulosa cells were negative for the staining. In preantral follicles, both the oocyte and granulosa cells were moderately immunostained for
myc protein.In antral and preovulatory follicles, there was no appreciable staining for
myc protein in the granulosa or theca cells, while
myc protein staining in the oocyte persisted with less intensity. It is of interest that
myc protein expression in granulosa cells was apparent only during the preantral follicle stage. Corpora lutea during the early and mid luteal phase were negative for
myc protein staining, whereas in regressing corpora lutea during the late luteal phase, peripheral theca lutein cells adjacent to the central core of scar tissue were immunostained for
myc protein. Corpora albicans showed no staining for
myc protein. In atretic follicles, granulosa cells and theca interna cells demonstrated positive staining for
myc protein. Ovarian stromal cells were negative for the immunostaining throughout the menstrual cycle. This demonstrates that
myc protein is expressed in a stage-limited manner in the human ovary during follicular growth and regression. The abundant expression of
myc protein in the oocyte at the primordial and preantral follicle stages and in the granulosa cells at the preantral follicle stage suggests a role for
myc expression in the initial growth of the oocyte as well as in the autonomous growth of granulosa cells during the preantral stage seemingly independent of gonadotropic stimulation. Furthermore, notable expression of
myc protein in the granulosa cells and theca interna cells of atretic follicles and in the peripheral theca lutein cells of regressing corpora lutea implies the possible participation of
myc expression in remodelling the ovarian local tissue following atresia and luteolysis in the human ovary.
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