我々は石炭酸フクシンの染色効果とくに東南アジア及び日本の癩施設に於て使用されている石炭酸フクシン溶液の中の塩基性フクシンの組成のそれについて研究した。
異った施設は異った染色法,hot又はcold, 546～556μmの吸収曲線をもった塩基性フクシンを使用,異った脱色剤,脱色時間を使用している。
低い吸収をもった石炭酸フクシンでは菌はBead状の染色を示し,これに反し,高い吸収性をもつフクシンではRod状を示す。だから異った施設で同一の結果を得ることは困難であり又BI及びMIも同様である。
この問題は石炭酸フクシン法によって染色されないchromophobic菌の存在により混乱する。従って古典的石炭酸フクシン法は感染性を決定したり治療を止めるためには信頼出来ない。
癩菌のBIとMIを決定するためには高い吸収をもつ塩基性フクシンを使用する染色法を標準化することが必須である。抗酸性とchromophobic菌の両者を染める過沃度酸・パラロザニリン染色はだからもっとも信頼出来,そして適切な方法である。

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Four cases with leprosy were treated with immunotherapy by means of injection of transfer factor (TF). In the first case, BI reached the level of 1 in 1975, which thereafter continued for three years despite of continuation of chemotherapy. And this case was treated with the transfusion of leucocytes. The immunotherapy by means of injection of TF started nine weeks after the termination of transfusion of leucocytes. Also in the next case, the number of leprosy bacilli decreased to a small number by chemotherapy, but could not reach the negetivity despite of continuation of chemotherapy. In the other two cases, the injection of TF started at the stadium in which the number of leprosy bacilli decreased to middle grade by chemotherapy. The type of eprosy was BL in three cases, and LL/BL in a case.
TF was extracted from the leucocytes of normal healthy adults. One vial of TF was injected once or twice weekly, or two vials of TF were injected once weekly. The total number of times of injection of TF is 25 in the first case, and 31 in the 2 nd case. And the total amount of TF is 38 vials in the first case, and 45 vials in the 2 nd case. In the other 2 cases, the number of times was 16 and 13 respectively, and the total amount was 16 vials and 13 vials respectively.
The reversal reaction did not occur in every cases. The tendency of decrease of number of leprosy bacilli was somewhat observed in the first 2 cases, but not observed in the other 2 cases. In a case of first two cases, the number of T-cell increased a little.The function of lymphocytes did not change by the injection of TF. The lepromin reaction of first two cases was examined. In a case, the marked but temporarily positive Fernandez reaction occurred, and the Mitsuda reaction changed from negative to doubtful reation. But despite of the injection of TF made many times, clearly positive Mitsuda reaction did not occur. The amount of immunoglobulin and complement did notchange.
Judging from the data obtained from this study, big hope cannot be placed on TF.

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