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Volume 33, Issue 4
Displaying 1-27 of 27 articles from this issue
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Kazuya Miyoshi, Sadanori Fuchimoto, Toshihide Ohsaki, Tatsuhiko Sakata ...2000 Volume 33 Issue 4 Pages 427-432
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSThe objective of this study was to evaluate which reconstruction method results in the best quality of life after total gastrectomy. A total of 48 patients underwent the following reconstructions: pouch and Roux-en-Y (PRY)(N=23), pouch and interposition (PI)(N=12) or Roux-en-Y without pouch (RY)(N=13). The subjective symptoms, the quantity of meals, the body weight, the serum nutritional parameters and the retention rate of isotope labeled meal in correlation to reconstruction type were documented. Twelve months after operation, PRY and PI groups were found to have significantly better quantity of meals (p<0.05) and higher body weight. However, a few patients in the PI group complained of refractory postprandial symptoms (vomiting or regurgitation) and showed severe retention of isotope in the pouch. We conclude, PRY is the better reconstruction method than PI and PRY within the first 12 months after total gastrectomy.View full abstractDownload PDF (81K) -
especially, Gastric Carcinoma with Submucosal (sm) InvasionYoshihiro Kagawa, Kazuhiro Yoshida, Naoki Nishimoto, Yasuo Yano, Toshi ...2000 Volume 33 Issue 4 Pages 433-439
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSIn this study, we analysed the relationship between the expression of cell cycle regulators and clinicopathological parameters of gastric carciomas. We examined the expression of p27Kip1 (p27), cyclin E, p53 and p21WAF1 (p21) in gastric carciomas, especialy in order to predict lymph node metastasis of gastric carciomas with sm invasion. One hundred and seventy eight gastric adenocarcinomas were examined and Immunohistochemical staining was performed with p27, cyclin E monoclonal antibodies. Sixty nine gastric carciomas with sm invasion were also stained with p53, p21 monoclonal antibodies. The relationship between the grade of expression and clinicopathological parameters was examined. Low grade expression of p27 was detected in undifferentiated type, the cases with lymph node metastasis, and advanced stage. Moreover, low grade expression of p27 and high grade expression of cyclin E had a strong correlation with lymph node metastasis. The prognosis of these patients had no correlation with grade of expression of p27 or cyclin E. On the other hand, in gastric carciomas with sm invasion, high grade expression of p27 correlated with differentiation and lymph node metastasis. High grade expression of cyclin E and p21 also showed correlation with lymph node metastasis. Further analysis should be performed in order to predict lymph node metastasis of gastric carcinomas with sm invasion.View full abstractDownload PDF (66K) -
Multivariate Analysis of Risk Factors Using Logistic RegressionFumiro Mochizuki, Masashi Fujii, Yuichi Kasakura, Tetsuro Suzuki, Nori ...2000 Volume 33 Issue 4 Pages 440-447
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSThe purpose of this study was to review the risk factors of recurrence of early gastric cancer. Between 1974 and 1997 1013 patients with early gastric cancer underwent surgical resection in our department, and 24 of them (2.4%)(one of the 455 [0.2%] with mucosal cancer, 23 of the 558 [4.1%] with submucosal cancer) had a recurrence. The 24 patients were analyzed with regard to location of the recurrence and postoperative interval to the recurrence, and they were compared with the no-recurrence group in terms of sex, age, location, gross appearance, tumor size, operative procedures, histology, depth of invasion, INF, lymph-vascular invasion, lymph node involvement, and multiple lesion. Analysis using the chi-square test suggested that large tumor size, undifferentiated type, submucosal invasion, positivity for lymph-vascular invasion, lymph node metastasis, and multiple lesions were significantly related to recurrence. On the other hand, analysis by logistic regression suggested that undifferentiated type, submucosal invasion, lymph node metastasis, and multiple lesions were significantly related to recurrence. It also suggested that among early gastric cancers, those with 2 of the 4 risk factors (submucosal invasion, undifferentiated type, lymph node metastasis, and multiple lesions) had a greater tendency to recur by a hematogenous spread, and that those with upper 3 risk factors should be treated as an advanced cancer such as D2 lymph node dissection and adjuvant chemotherapy.View full abstractDownload PDF (55K) -
Kimiaki Hirama, Takayuki Morita, Mitsuru Konn2000 Volume 33 Issue 4 Pages 448-454
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSWe studied the clinical significance of histological heterogeneity (the histological combination in a single tumor) in colorectal cancer, and examined immunohistologically the expression of urokinase-type plasminogen acticator (uPA). The patients were less than 75 years, and had undergone curative surgery for advanved colorectal cancer. Their cases were investigated for the relation between histological heterogeneity and clinicopathological factors, and the influence on disease free 5-year survival rate and recurrent form. Furthermore, uPA expression was examined immunohistologically. The positive cases of histological heterogeneity showed poor prognoses in disease free 5-year survival rate. Histological heterogeneity related to local recurrence in colon cancer. UPA showed no significance in relation to histological heterogeneity. We speculated that the histological heterogeneity reflected biological malignancy of tumors and proved to be an important prognosis detecting factor.View full abstractDownload PDF (102K) -
Mitsunobu Kikuchi2000 Volume 33 Issue 4 Pages 455-461
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSTwenty four patients were involved in a study on defecation function of the patients undergoing sigmoidectomy or anterior resection of the rectum. Clinical bowel function was determined from the point of frequency of defecation and soiling. Defecography was performed in 24 patients with anterior resection of rectum or sigmoidectomy. There was an inverse correlation between anastomoses situated above the peritoneal reflex and bowel movement(p<0.01). The anastomotic sites of seven patients who suffered from soiling were all within 1 cm above the anal canal. There were no difference between the anorectal angle and perineal descent atrest, during squeezing and straining before and post operation. Rectal emptying ratio after the operation was significantly lower than that before the operation. There was an inverse correlation between the rectal emptying ratio and bowel movement 1 year after operation(p<0.05). The rectal emptying ratio was 66±35% in the patients without soiling, 38±30% in the patients with soiling. Videodefecography revealed that barium below the anastomosis was evacuated, but barium above the anastomosis remained in the colon. Therefore, poor rectal emptying was mainly due to poor rectal emptying of the contents above the site of anastomosis. After self-irrigation of the colon, defecation disorders such as analgia, perianal redness, sleeplessness and soiling improved in all patients. In conclusion, increase of bowel movement after sigmoidectomy or anterior resection of rectym, was due to poor emptying of the rectum, especially above the site of the anastomosis.View full abstractDownload PDF (69K) -
Shoichi Tsukayama, Makoto Hirano, Nozomu Murakami, Yusuke Uno, Hiroshi ...2000 Volume 33 Issue 4 Pages 462-466
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSWe report a case of basaloid-squamous carcinoma of the esophagus in a 70-year-old man with a chief complait of epigastric discomfort. A barium esophagogram showed a filling defect, 4-cm in length, in the midportion of the esophagus. Endoscopic examinations revealed a lobular, protruding tumor with an ulceration on its surface (34 cm distal to the incisirs). Biopsy of the tumor showed moderately differentiated squamous cell carcinoma, and surgery was performed. The resected specimen showed a type 1 lesion, 4.0×3.5 cm in diameter. The histopathological diagnosis was esophageal basaloid-squamous carcinoma. The cancer cells formed solid sheets with squamous differentiation and pseudo-glandular appearance, and the tumor exhibited submucosal expansion with normal esophageal epithelium on its surface and had slightly invaded the muscularis propria. The prognosis of esophageal basaloid-squamous carcinoma is considered to be very poor. The patient died of liver metastasis 9 months later. PyNPase, a known angiogenic factor, was highly expressed in the cancer cells. This suggests a relation with the malignant potential of esophageal basaloid-squamous carcinoma.View full abstractDownload PDF (95K) -
Takashi Sakurai, Shigeyuki Kawachi, Takaaki Yamamoto, So Inoue, Hideyu ...2000 Volume 33 Issue 4 Pages 467-471
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSGastrocolic fistulas are rare complication. A 60-year-old male of this disease following gastroduodenal anastomosis was successfully treated by laparoscopic fistulectomy and omental implantation. Operative time was 4 hr 32 min, and blood loss was little. To recognize the fistula, the light of intraoperative gastrofiberscope was very useful. Omental implantation was technically easy, rare to make stenosis and not needed to take postoperative anti-acidic drugs. The postoperative period was uneventful, and now the patient is good condi tion without any medications. Although prior abdominal surgery has been felt to represent a relative contra indication to laparoscopic surgery, a laparoscopic approach to cases such as described here seems to be tech nically feasible and beneficial.View full abstractDownload PDF (106K) -
Tetsuya Kajimoto, Naoshi Tashiro, Masaru Naruse, Toshihiko Sasaki, Ryu ...2000 Volume 33 Issue 4 Pages 472-476
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSA 52-year-old male was hospitalised because of melena and general fatigue. Endoscopic examination revealed a protruding lesion resembling a submucosal tumor which was accompanied by an ulcer opposite the major papilla of the duodenum. The tumor was also observed on abdominal computed tomography and hypotonic duodenographic examination in the descending part of the duodenum. Endoscopic ultrasonography showed that the tumor was derived from the muscle layer of the duodenal wall. In consideration of these findings and angiographic evidence in which enlargement, encasement and hypervascularity of the feeding artery to the tumor was observed, we diagnosed the tumor as a leiomyosarcoma of the duodenum. Partial resection of the duodenum including the tumor, which showed exoenteric growth along the descending and transverse part of the duodenum, was performed. The tumor was 64mm long and associated with an ulcer on the mucosa. The inside of the tumor had an irregular and cystic appearance, which was thought to be because of necrotic changes. Histological examination demonstrated leiomyosarcoma. In conclusion, we need to consider leiomyosarcoma when a duodenal tumor is found and to perform the above examinations. Among the examinations, endoscopic ultrasonography is thought to be the most useful one for diagnosis of duodenal leiomyosarcomacoma and, therefore, we suggest that endoscopic ultrasonography should be performed if possible.View full abstractDownload PDF (112K) -
Yoshifumi Nakayama, Koji Kadowaki, Naoki Nagata, Yoshiaki Hirano, Hide ...2000 Volume 33 Issue 4 Pages 477-481
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSA 56-year-old man undergoing hemodialysis had been regularly examined with endoscopy in another hospital hospital because of gastric adenoma after endoscopic polypectomy. Hypotonic duodenography and endoscopy detected a depressed type tumor with redness and marginal elevation, mesuring 6×4mm in size, in the second portion of the duodenum. The histological examination of the biopsy specimens disclosed well-differentiated adenocarcinoma. Because of a failed endoscopic mucosal resection, a wedge resection of the duodenum was performed. The resected specimen proved to be a IIc+IIa type early duodenal cancer. Histological examination revealed a well differentiated adenocarcinoma restricted to the mucosal layer, and the resected margins were tumor-free. This case was the 25th case of depressed early duodenal cancer, and the first case in a hemodialysed patient.View full abstractDownload PDF (84K) -
Johta Watanabe, Takashi Nishizaki, Kenzo Wakasugi, Ikuo Takahashi, Tos ...2000 Volume 33 Issue 4 Pages 482-486
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSA 66-year-old woman complained of epigastralgia about one month prior to consulting our clinic. Endo-scopic examination revealed a tumor about 10 mm in diameter in the ampulla of Vater. A biopsy specimen revealed adenocarcinoma, and pancreatoduodenectomy was performed. The resected specimen showed well differentiated adenocarcinoma of the papilla of Vater and another tumor 4 mm in diameter in the accessory papilla. In the immunohistochemical analyses, the cytoplasm of the cells in the tumor in the accessory papilla papilla stained positive staining for neuron specific enolase (NSE) and chromogranin-A (CgA). The diagnosis was carcinoid. At the 30-month follow-up examination the patient was doing well with no evidence of recurrence of either the carcinoma or the carcinoid. To the best of our knowledge, this is the first case in the literature of concomitant adenocarcinoma of the papilla of Vater and carcinoid tumor of the accessory duodenal papilla.View full abstractDownload PDF (115K) -
Hiroshi Itoh, Ryouhei Izumi, Hisashi Hirosawa, Naotaka Kadoya, Wataru ...2000 Volume 33 Issue 4 Pages 487-491
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSA 24-year-old man who complained of right upperquadrant pain was admitted to our hospital. Biochemical studies showed elevations of carcinoembryonic antigen and alpha fetoprotein. Abdominal ultrasonography showed a well defined mass in the right lobe of the liver with multicystic areas. Contrast-enhanced computed tomography (CT) revealed a well-circumscribed enhancing tumor approximately 15 cm in diameter with focal low attenuation areas. Selective arteriography of the celiac axis demonstrated a hypervascular tumor in the right lobe of the liver and an arteriovenous shunt. CT during arterial portography (CT-AP) revealed a defect in portal flow from the superior mesenteric artery and some revealed entrapped branches of the portal vein in the area. With a preoperative diagnosis of hemangiosarcoma of the liver, right hemihepatectomy with resection of adherent diaphragm was performed. Macroscopic examination of the resected specimen showed a solid tumor with sharp demarcation between the tumor margin and adjacent normal liver. The cut surface of the resected tumor was variegated, predominantly tan-yellowish, and exhibited cystic areas of variable size that contained hemorrhagic fluid and gelatinous material with focal necrosis. Histologically, the major compo nent of the tumor was sarcoma, dilated epithelium-lined structures were present, there were no differentiated components, and the tumor contained cells with intracytoplasmic hyaline globules that were periodic acid Schiff positive, findings which were consistent with undifferentiated sarcoma of the liver. Immunohistochemi-cally, strong CD34 immunoreactivity was observed in the cytoplasm of the tumor cells, but no markers of histiocytes, neurons, muscle-specific actin, or vessels were detected. CT-AP and immunohistochemical studies may be new diagnostic methods for this rare tumor.View full abstractDownload PDF (108K) -
Haruhiro Nakazaki, Masashi Watanabe, Yukitake Hasebe, Natuki Tokura, M ...2000 Volume 33 Issue 4 Pages 492-496
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSA 61-year-old male was admitted for chronic myelocytic leukemia. Computed tomography showed a tumor 4cm in diameter in the left lobe of the liver and a tumor 8mm in diameter in the right lobe. The serum alfa-fetoprotein (AFP) level was 1, 757ng/ml. Left hepatic lobectomy and ethanol injection for a tumor in S8 were performed in October 1994. Multiple bilateral lung metastases were detected by chest X-P and CT scan in April 1996. Then CDDP, 20mg (iv; for 1hour), and 5-FU, 2, 000mg (iv; continous for 7days) were then administered repeatedly. Six months after the administration, the AFP levels had decreased to 340ng/ml from 5, 205, and lung metastases had disappeared, except a tumor 1.5cm in diameter in the left lung. As the tumor failed to resolvedid in spite of additional chemotherapic, wedge excision of the left lung was performed. He is alive without recurrence in lung and liver. We have reported a case of HCC in which resection of a pulmonary metastases was performed after systemic chemotherapy.View full abstractDownload PDF (128K) -
Keiichi Suzuki, Shin Takahashi, Kouichi Aiura, Junichi Saitou, Shigeo ...2000 Volume 33 Issue 4 Pages 497-501
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSFive cases of adenosquamous cell carcinoma of the pancreas were studied in our hospital between January 1982and June 1998. The age range was from 43to 78years with a mean of 64years. Four patients were male and one patient was female. The locations of the tumor were the pancreatic body-tail in 4patients and the pancreatic head in one patient. Operations were carried out in all patients, and all cases were resectable. The tumor sizes were larger than ts3 (>4.0cm and ≤6.0cm) in all patients. In only one patient, the angiogram defected the tumor by its stain of hypervascularity. All patients were in the progressive stage (more than stage IV), and all died within 1year, except the latest case who died after 15months. Adenosquamous cell carcinoma of the pancreas is difficult to diagnose, so identification at the early stage is most important because of poor prognosis.View full abstractDownload PDF (77K) -
Takafumi Oshiro, Toshiyuki Itamoto, Takayuki Kadoya, Yasuhiro Fudaba, ...2000 Volume 33 Issue 4 Pages 502-506
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSWe report here a rare case of epithelioid leiomyosarcoma found in the colon. A 59-year-old male was admitted to our department with a pain in the upper abdomen. Total colonoscopy showed a tumor with ulceration in the transverse colon, and CT scanning demonstrated a large mass with low density areas attaching to thicked great curvature wall of the stomach extending from the body to antrum. He underwent partial resection of the transverse colon and stomach to remove an 8.0×7.5×6.0cm tumor of the transverse colon, which invaded to the stomach and metastasized to the lymph nodes of the middle colic artery and in the great omentum. Histopathological examination of the tumor showed that reticular network was formed by round or polygonal cells with eosinophilic spacious cytoplasm and highly pleomorphilic nuclei. Immunohistologic assessment revealed that most of the cells were positively stained for smooth muscle actin, and a diagnosis of epithelioid leiomyosarcoma was made. Surgical removal is selected as the primary treatment of this tumor, but poor prognosis has been reported on this disease, especially the tumor originated from the colon.View full abstractDownload PDF (92K) -
Mutsumi Nozue, Tsunehiko Maruyama, Fumito Imamura, Masataka Fukue, Kou ...2000 Volume 33 Issue 4 Pages 507-511
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSTo prepare for the change in the health insurance system in Japan, critical path guidelines have been introduced to our hospital. In our surgical department, critical path for distal gastrectomy has also been inducted since autumn in 1998. To evaluate the effect of the induction, days in hospital and points for health insurance were compared between 10cases treated using the critical path guidelines and 17cases without critical path. The path prescribes the clinical course as follows: preoperative exam at outpatient clinic, 2days stay befor operation, 16days stay after operation. As a result, about 70% of patients followed this path. The average hospital stay has significantly decreased from 36to 27.3days after induction of the path. Average points for health insurance also significantly decreased from 165, 800to 133, 900. Adjusting points by days in hospital to 19days, showed that the medical care itself was also standardized under the critical path.View full abstractDownload PDF (150K) -
Katsuyuki Kunieda, Hisashi Kohmura, Jun Sano, Kazuya Yamaguchi, Shinic ...2000 Volume 33 Issue 4 Pages 512-516
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESS[Purpose] To facilitate identification of a high risk group of colorectal cancer patients with liver metastasis, we studied the relationship between gene products and liver metastasis by the PCR method and immunohistochemical staining.[Results] 1) Expression of MMP-9, uPA, and TIMP mRNA as assessed by RT-PCR was studied in relation to liver metastasis in 48colorectal cancer patients. The incidence of gene expression of MMP-9and uPA was significantly higher in cases with liver metastasis, and MMP-9 (+) and uPA (+), and MMP (+), and TIMP (-), were significantly linked to metastatic spread to the liver. 2) Immunohistochemical expression of p53, c-erbB-2, CD44, nm23, and PCNA-LI was compared in 33cases with metachronous liver metastasis (metastasis group) and 66cases without liver metastasis (control group), and the incidences of CD44 (+), nm23 (-), and PCNA-LI≥60were found to be significantly higher in the metastasis group than in the controls. 3) Study of angiogenic factors also showed VEGF and MVD to be significantly associated with liver metastasis. 4) Detection of free cancer cells in mesenteric veinous blood draining tumors by PCR using CEA and CK-20primers showed that the positive rates increased with clinical stage. Seven patients in the group without obvious liver metastasis were positive, and metachronous liver metastasis was observed in 2of them.[Conclusions] MMP-9, uPA, TIMP, VEGF, PCNA, nm23, and CD44have significant correlations with liver metastasis by colorectal cancer, and analyzing them is considered useful for identification of cases at high risk of liver metastasis along with use of the PCR method to detect free cancer cells in the mesenteric veins draining tumors.View full abstractDownload PDF (50K) -
Naohiro Tomita, Masatomo Tada, Mitsugu Sekimoto, Masayuki Ohue, Tadash ...2000 Volume 33 Issue 4 Pages 517-522
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSIn the operation for advanced rectal cancer, some cancer cells may possibly emigrate from lateral bloodor lymph-vessels during mobilization of the rectum. This could be related to lateral lymphnode metastasis or local recurrence, although its detail is quitely unknown. In order to clarify this point, we tried to detect cancer cells in pelvic lavage fluid during rectal surgery by RT-PCR analysis using a new epithelial cell-specific gene marker, GS04094 (Cancer Res., 58, 2440-2444, 1998). During the operation for rectal cancer, pelvic lavage with 100ml saline was performed before rectal manipulation (pre) and after complete mobilization of rectum (post), and each sample was then examined by cytologic and RT-PCR analyses. Among 14cases of rectal cancer, 5 cases were shown to be positive by RT-PCR analysis indicating the possible presence of cancer cells. These included 4cases with pre (+) and post (+), and 1case with pre (-) and post (+). A correlation was demonstrated between RT-PCR positive diagnosis and depth of tumor invasion, lymph node metastasis, lymphatic invasion, or tumor location (only for pre (+) cases). These preliminary results suggest that the emigration of cancer cells by breakdown of lateral vessels or the leakage of cancer cells to the serosal surface could be detected with high sensitivity using RT-PCR analyses of GS04094on pelvic lavage samples during operations for rectal cancer. This indicates that RT-PCR analyses may be useful for tumor staging and/or the determination of therapeutic procedures.View full abstractDownload PDF (79K) -
Analysis of Factors associated with Peritoneal Metastatic Ability of Scirrrhous Gastric Cancer CellsTohru Inoue, Masakazu Yashiro, Shigehiko Nishimura, Tasuku Matsuoka, Y ...2000 Volume 33 Issue 4 Pages 523-528
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSThe mechanisms responsible for peritoneal metastasis are not well understood. We established a high-peritoneal-metastasis cell line (OCUM-2MD3) from a poor-peritoneal-metastasis gastric cancer cell line (OCUM-2M). Factors associated with peritoneal metastasis were analyzed by comparing the characters of the two cell lines. The process of peritoneal metastasis has comprised several steps. When cancer cells invade through the gastric wall, matrix metalloproteinase-1is an important factor. When cancer cells escape from primary tumor into peritoneal space, decreased E-cadherin expression is a favorable event for cancer cell dissociation. CD44H was associated with attachment between cancer cells and mesothelial cells.α2β1-integrin and α3β1-integrin were associated with attachment to the matrix of the peritoneal stroma. Peritoneal fibrosis induced by gastric cancer cells was a favorable environment for peritoneal metastasis. The above factors appear to be necessary to the ability to produce peritoneal metastasis.View full abstractDownload PDF (129K) -
Shinji Tanaka, Koichi Sato, Masaki Mori, Keizo Sugimachi2000 Volume 33 Issue 4 Pages 529-532
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSA novel member of the human frizzled (Fz) gene family was cloned and found to be specifically expressed compared to the adjacent uninvolved normal mucosa in 28of 47 (60%) squamous cell esophageal carcinomas. The FzE3cDNA encodes a protein of 574amino acids and shares high sequence homology with other frizzled genes, particularly in the putative ligand-binding region of the cysteine-rich extracellular domain. Functional analysis revealed that transfection and expression of the FzE3cDNA in esophageal carcinoma cells stimulates complex formation between APC and β-catenin followed by nuclear translocation of β-catenin, which mediataes cell-cell attachment with E-cadherin. Furthermore, cotransfection of a mutant construct encoding a FzE3protein with a C-terminal truncation completely inhibited the interaction of APC with β-catenin in the cells. These observations suggest that FzE3gene expression may downregulate APC function and enhance β-catenin mediated signals in human esophageal carcinomas.View full abstractDownload PDF (59K) -
Yoko Kondo, Shigeki Arii, Akira Mori, Masayuki Imamura2000 Volume 33 Issue 4 Pages 533-536
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSExpression of vascular endothelial growth factor (VEGF) in 133colorectal tumor specimens comprised of 27adenomas, 46early colorectal carcinomas, 60advanced colorectal carcinomas was evaluated by RT-PCR, Northern blot hybridization, and was immunohistochemistry. Tumor angiogenesis paralleled tumor development, and partially associated with VEGF expression, in colorectal adenomas and the early stages of human colorectal cancer. Expression of VEGF mRNA in advanced colorectal carcinomas was correlated with tumor progression, including metastasis. We attempted to clarify the role of VEGF in the tumor growth, neovascularization, and metastasis of human colon cancer by using cells transfected with the VEGF gene. We found that sense-VEGF transfectants have a growth advantage due to vigorous tumor angiogenesis. We also demonstrated that sense-VEGF transfectants metastasized to the liver and had greater potential to induce peritoneal dissemination. In conclusion, the present study provided clear evidence that VEGF plays an important role in cancer growth by stimulating neovascularization and subsequent metastasis.View full abstractDownload PDF (29K) -
Michiyoshi Hisanaga, Yoshiyuki Nakajima, Mitsuo Nagao, Hiromichi Kaneh ...2000 Volume 33 Issue 4 Pages 537-542
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSWe investigated the expression of Fas receptor (Fas)/ligand (FasL) in 47patients with hepatocellular carcinoma (HCC). Fas and FasL expression were detected in 16patients and 19patients by immunohisto-chemistry and RT-PCR, respectively. An inverse relationship was noted between Fas and FasL expression in HCCs. In noncancerous liver tissue (n=37), Fas expression on the cell surface was upregulated in 18cases, and FasL expression was detected in 6cases. In the Fas-positive cases, there was more tumor cell apoptosis, and the disease-free survival rate was higher, compared with the Fas-negative cases. The soluble Fas (sFas) and soluble FasL (sFasL) levels of the HCC patients were significantly higher and lower, respectively, than in healthy volunteer. Generation of sFas was detected in the hepatocytes and tumor-infiltrating cells rather than in the hepatoma cells. These results indicate that hepatoma cells may stop expressing Fas on themselves and let the hepatocytes and tumor-infiltrating cells generate sFas to escape the host immune system and to produce metastasis. The fact that hepatocytes in the pericancerous pseudolobules expressed FasL suggests that FasL may contribute to malignant transformation and carcinogenesis.View full abstractDownload PDF (54K) -
Shuji Hayashi, Akimasa Nakao2000 Volume 33 Issue 4 Pages 543-548
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSHepatic metastasis is an important factor to limit the prognosis of patient with abdominal malignancies. In this study, the authors examined the effect of gene therapy on the inhibition of hepatic metastasis after abdominal surgery using adenoviral vectors. After adenoviral vectors with tissue plasminogen activator (tPA), interleukin-10 (IL-10), and I Kappa beta (IKB) cDNAs were transduced to the liver and/or cancer cells, the experiments on hepatic metastasis were performed. The findings demonstrated that adenovirus-mediated gene transfer with tPA or IL-10cDNA was effective in the suppression of hepatic metastasis. especially by transduction to both the liver and cancerous tissue, and that hepatic metastasis was synergistically inhibited by using both IKB cDNA and CDDP. In conclusion, gene therapy for the liver and cancer tissue is not only feasible but also a novel strategy to inhibit hepatic metastasis after abdominal surgery.View full abstractDownload PDF (70K) -
Tetsuji Sawada, Hitoshi Teraoka, Tamahiro Nishihara, Takeshi Sunami, M ...2000 Volume 33 Issue 4 Pages 549-553
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSWe investigated the biological role of the expression of ICAM-1, which is an adhesion molecule involved in host immunodefense in the microenvironment, and of TGF-β1in the liver metastasis ability of pancreatic cancer cells. The highly metastatic, SW1990cells in a nude mouse liver metastasis model had the lowest expression of ICAM-1, and the adhesiveness and cytotoxicity of mononuclear leukocytes against SW1990were suppressed. Treatment of pancreatic cancer cells with TGF-β1decreased their expression of ICAM-1and increased their invasiveness, and it enhanced the in vivo liver metastasis ability of SW1990and CAPAN-2. These findings suggested that decreased cancer cell expression of ICAM-1induced by various factors, such an TGF-β1, plays an important role by allowing cancer cells to escape from the immune defense system, and ICAM-1gene transfection or anti-TGF-β1antibody may be applicable as a means of inhibiting metastasis.View full abstractDownload PDF (68K) -
Hiroshi Egami, Naoko Hayashi, Takashi Kurizaki, Mikio Kai, Eiji Takai, ...2000 Volume 33 Issue 4 Pages 554-559
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSPancreatic cancer has the worst prognosis of any neoplasm, one reason being that it has an extremely high potential for invasion and metastasis. Two pancreatic cancer cell lines, the highly invasive and metastatic cell line PC-1·0and the weakly invasive and rarely metastatic cell line PC-1, were established from a pancreatic ductal carcinoma induced by N-nitrosobis (2-oxopropyl) amine in a Syrian golden hamster. A soluble proteinous factor called dissociation factor (DF), which can induce dissociation of cancer cell colonies was found in PC-1·0-cell conditioned medium. DF has been partially purified, and analysis of its amino acid sequence revealed that DF is a protein with an amino acid sequence similar to that of 1) a new type of metalloproteinase, or 2) Fc binding protein with mucin-like structure, with an approximate molecular weight of 50kD. Study of its biological activity showed that partially purified DF can induce not only cancer cell dissociation, but cell motility, selective cell adhesion to fibronectin, and chemoinvasion in vitro. Based on the results of this study, DF seems likely to have a role in cancer cell invasion and metastasis in pancreatic cancer.View full abstractDownload PDF (93K) -
Katsumichi Iki, Toshimitsu Majima, Tadahiko Kubozoe, Sueharu Iwamoto, ...2000 Volume 33 Issue 4 Pages 560-563
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSIn the present experiment, the effects of OPB-3206, 3S-[4-(N-hydroxyamino)-2R-isobutylsuccinyl] amino-1-methoxy-3, 4-dihydrocarbostyril, a novel metalloproteinase inhibitor, were studied on the growth of hamster pancreatic ductal carcinomas induced by a rapid production model. OPB-3206inhibited the activities of interstitial collagenase, gelatinase A and B, and stromelysis in vitro. When 0%, 0.1% and 0.2% OPB-3206 was orally administered in the diet for 7weeks, the numbers of pancreatic nodules were significantly reduced in administered group from 46days after BOP initiation onward. Zymographic analysis showed that the gelatinolytic activity of proMMP-2and activeMMP-2was detected in control group. On the 0.1and 0.2% OPB-3206diet proMMP-2activity was increased, but that of activeMMP-2became undetectable. The results thus suggest that OPB-3206selectively inhibits carcinogenesis in hamster pancreas by inhibiting MMP-2 activation.View full abstractDownload PDF (50K) -
Seiji Marubayashi, Kazurou Okada, Kazuyuki Fukuma, Yoshio Oshiro, Hiro ...2000 Volume 33 Issue 4 Pages 564
Published: 2000
Released on J-STAGE: June 08, 2011
JOURNAL FREE ACCESSDownload PDF (22K) -
[in Japanese], [in Japanese], [in Japanese]2000 Volume 33 Issue 4 Pages 565-566
Published: 2000
Released on J-STAGE: June 08, 2011
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