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Volume 19, Issue 3
Displaying 1-9 of 9 articles from this issue
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Munetada Oimomi1990 Volume 19 Issue 3 Pages 257-265
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
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III. Sample and MethodJihei Iwata, Tetuya Suga1990 Volume 19 Issue 3 Pages 266-274
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSTo find an adequate pretreatment method of urine and serum, and to apply the methodfor the determination of 170S conjugates proposed previously to the field of clinical chemistry, we examined pretreatment of urine and serum samples. On the basis of results of the present study and the previously proposed methods, we developed the method for the determination of four 17OS sulfates and seven glucuronides in urine and four 170S sulfates in serum.View full abstractDownload PDF (865K) -
Minoru Tozuka1990 Volume 19 Issue 3 Pages 275-281
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSApo (E-AII) complex (a disulfide complex of monomers of apoE and ApoAn) and apoE in human apoVLDL showed microheterogeneity in molecular weight and isoelectric point on two dimensional gel electrophoresis. It was found that the apo (E-MI) complexes reveal isoforms related to apoE phenotypes and a minor portion of the complexes and apoE constitute sialylated isoforms. In the two dimensional gel electrophoretic patterns on phenotypes of E3/E3 and E2/E2, apo (E3-A11) complex or apo (E2-A II) complex with its sialylated isoforms were observed, whereas in phenotypes of E3/E2, both apo (E-A II) complex isoforms were found. In phenotypes of E4/E3 or E4/E2, only a band of apo (E3-AII) complex or apo (E2-A II) complex was found, with relatively poor intensity, and no apo (E-AII) complex was detected in phenotype of E4/E4, probably because of the absence of cysteine residue in the apoE4 molecule.View full abstractDownload PDF (8258K) -
Yasushi Ikarashi, Kouichi Itoh, Kenji Matsuura, Yuji Maruyama1990 Volume 19 Issue 3 Pages 282-289
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe have successfully determined the levels of acetylcholine (ACh) in seven rat brain regions by LC/MS equipped with direct sample introduction with splitting capability and a frit-fast atom bombardment (FRIT-FAB) ionization source system. Base peaks of the molecular ions of m/z 146 for ACh and m/z 150 for ACh-d4, the internal standard, were exhibited in the FRIT-FAB spectra with a small amount of fragments. Without requiring additional sample preparation or derivatization, the direct simultaneous determination of these ions by mass chromatography allowed detection of ACh levels as low as 1.0 pmol. The ACh levels measured by this approach were compared with those measured by conventional pyrolysis-GC/MS and LCEC. The similar ACh levels in the individual regions and their order were in accord with those determined by these three methods.View full abstractDownload PDF (774K) -
Rinzo Uenoyama, Jun Chuma, Kazufumi Suzuki, Fumihiko Takeda, Shu Haseg ...1990 Volume 19 Issue 3 Pages 290-299
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe describe the new test paper method for determining total serum bile acid (TBA). The test paper includes sodium oxamate, EDTA 2Na, 3α-HSD, NAD, diaphorase, NTB and buffer as a reaction mixture. Determination was performed employing blank test paper identical to our experimental paper except that it had no 3α-HSD
The reproducibility of this method was 10% (CV) around 10 μM and the recovery, between 80 and 111%. The coefficient of correlation to Mashige's method (Enzabile 2, Daiichi Pure Chemicals Co., Ltd.) was 0.993. Hemoglobin affected the present measurement system, whereas bilir- ubin, LDH, ascorbic acid and Intrafat did not.
TBA was estimated by this method in 23 normal subjects and 89 patients with various liver diseases. Mean normal value was 4.1 μM. TBA levels in patients with chronic active hepatitis and liver cirrhosis were more than 10μM even if their serum bilirubin levels were below 1.0mg/dl. Thus, we recommend this determination system for the massscreening of chronic liver diseases.View full abstractDownload PDF (974K) -
Makoto Matsushita, Tsutomu Irino, Yoshimi Muramoto, Atsushi Kushigemac ...1990 Volume 19 Issue 3 Pages 300-306
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSA colorimetric method was developed for determining total proteins in serum using a nickel/biuret reagent. It is based on measurement of a orange-colored complex produced by reactions of peptide bonds with nickel ions in alkali. Eighteen nonprotein compounds were tested, and this proposed method gave color development with glutathione, L-histidine, L-cysteine, and L-leucinamide, the measuring absorbance at 500 nm. The Cu/biuret method, so called the biuret method, however, reacted with all compounds, such as L-threonine, L-serine, D-mannose, D-galactosamine, sialic acid, and others. The correlation between the two methods (x and y) was r=0.996, and the regression, y=1.015x-0.18. The mean value by the present method was about 0.07g/dl less than that by the Cu/biuret method, this difference being statistically significant (p<0.001, t-test). It these appears that this method may be more specific for determining total serum proteins than the Cu/biuret method.View full abstractDownload PDF (652K) -
Shinobu Hayakawa, Tetsuo Hayakawa1990 Volume 19 Issue 3 Pages 307-314
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSMonoclonal antibodies were raised against human pancreatic elastase 1 and used for a one step enzyme immunoassay. The α1antitrypsin-elastase 1 complex was employed as the standard in the assay. The assay's measurable range was 1.0-200ng/ml and the intra-assay coefficient of variation was 2.4-4.7%. Analytical recovery of the assay was 95.2±3.86%(x±SD). The average nomal serum level determined by the proposed method was 7.5ng/ml with a range of 2.9-19.2ng/ml. The enzyme immunoassay offers simple. rapid and specific analysis of serum pancreatic elastase 1 for clinical diagnosis.View full abstractDownload PDF (783K) -
Saburo Yamashita, Masahiro Miyashita, Koichiro Tsubokawa1990 Volume 19 Issue 3 Pages 315-321
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSCarboxyl terminal peptide bond was modified by thiohydantoin ring formation by the treatment of the C-terminal amino acid with trifluoroacetic anhydride and thiocyanate. The resulting modified peptide bond, peptidyl thiohydantoin, was then hydrolyzed by irradiating microwave (2450 MHz) in the presence of weak acid (2N HCl) for short duration (3 min). The thiohydantoin derivative of amino acid thus split was separated and identified by high performance liquid chromatography (HPLC). Microwave irradiation was found to be highly specific for the hydrolysis of the modified peptide bond and mild enough not to harm the residual peptide bonds.View full abstractDownload PDF (602K) -
Yoshiharu Kobayashi, Nami Saeki, Yoshino Nishiguchi, Fukuko Watanabe, ...1990 Volume 19 Issue 3 Pages 322-325
Published: September 30, 1990
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSDownload PDF (362K)
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