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Volume 24, Issue 1
Displaying 1-6 of 6 articles from this issue
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Kazuhisa Kanno, Kenji Tokunaga, Shuichi Saheki, Mitsuharu Murase, Nozo ...1995 Volume 24 Issue 1 Pages 1-6
Published: March 31, 1995
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSThe production of active oxygen species from neutrophils was compared between streptozotocin (STZ)-induced diabetic rats and control rats by using a flowcytometer with 2, 7-dichlorofluorescin diacetate as an indicator.
The production after stimulation with phorbol myristate acetate (PMA) was larger in neutrophils prepared from the diabetic rats than those from the control rats. On the 9th, 24th and 51st days after administration of STZ, the PMA-stimulated production of active oxygen species from neutrophils increased from the value 166.0±4.4 (before STZ) to 207.7± 4.9 (p<0.001), 24 days: 206.3±1.8, p<0.01 204.7±1.2 (p<0.05), respctively, in terms of peak channel (PC).
The increased capability of diabetic neutrophils to produce active oxygen species is assumed to play a role in the metabolic disorder of diabetes.View full abstractDownload PDF (636K) -
Yuko Ohashi, Masahiro Masada, Kane Shimamune, Hiroshi Naruse1995 Volume 24 Issue 1 Pages 7-11
Published: March 31, 1995
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSIt has been difficult to determine the actual level of tetrahydrobiopterin (BH4) in plasma because of its extreme instability. The ratio of BH4 to total biopterin (BH4%) in plasma also decreases with time by unknown decomposition. We found that the addition of some reducing agents was effective to prevent the decomposition. Based on of these findings, we developed a procedure to add a reducing agent (ascorbic acid) to the blood collecting equipment.
Neopterin, total biopterin levels and BH4% in plasma from 69 healthy adults and 35 patients with hepatitis C were determined by the method described above and were compared with normal control values. The neopterin level was significantly higher, and BH4% was significantly lower in almost all patients than in control subjects. These finding indicate that virus infection influences not only the level of neopterin but also the level of BH4 in plasma.View full abstractDownload PDF (607K) -
Yuji Suzuki1995 Volume 24 Issue 1 Pages 12-19
Published: March 31, 1995
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe examined the effects of electrolytes and non-electrolytes on the phenomenon of protein error of pH indicators, such as bromophenol blue and bromocresol green which have been commonly applied to the determination of protein in biological materials. Changes in optical absorbance due to protein error of the pH indicator decreased by the addition of various electrolytes (NaF, NaCl, NaBr, NaI, KF, KCl, KBr, KI, NH4Cl, Na2SO4,(NH4) 2SO4, MgSO4), but no change of absorbance was observed by the addition of non-electrolytes such as urea and glucose. The effects of different electrolytes on the change of absorbance were identical when the anion of electrolytes was the same. The effects of electrolytes also decreased with the increase in the pH of color reagents and the decrease in the ionic radius of the anion. The phenomenon of the protein error was confirmed to be closely related to the positively charged amino groups in protein molecule and dye anion.View full abstractDownload PDF (932K) -
Yoshifumi Matsuda, Hsien-Kung Lee, Izumi Hara, Hirohisa Sato, Teruko O ...1995 Volume 24 Issue 1 Pages 20-24
Published: March 31, 1995
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe measured the sperm count, and protein and tissue plasminogen activator (t-PA) levels in human semen samples from 13 individuals (aged 28 to 40 years). The mean volume of the human semen samples was 4.1±1.6ml (mean± SD), ranging from 1.8 to 6.6ml. The average protein concentration was 22.3±13.6g/l semen, but the values showed a wide distribution ranging from 7.2 to 59.4g/l semen. The t-PA antigen concentration and total amount of t-PA antigen in the human semen samples were 0.466±391mg/l semen and 2.194±2.531 μg/semen sample, respectively, and they showed a distribution ranging from 0.145 to 1.650mg/l semen and 0.370 to 9.900 μg/semen sample, respectively.
Strong correlations were observed between the total t-PA antigen amount and total sperm count or total protein amount (p<0.01), and a good correlation was also observed between t-PA antigen concentration and sperm count (p <0.05). However, the protein concentration and t-PA antigen concentration showed a weak correlation.View full abstractDownload PDF (616K) -
Nobuo Okumura, Fumiko Terasawa, Minoru Tozuka, Tsutomu Katsuyama, Keni ...1995 Volume 24 Issue 1 Pages 25-31
Published: March 31, 1995
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe assayed three different parameters of fibrin and/or fibrinogen degradation products (FDP), i. e. FDP-T, FDP-E, and FDP-DD, using an automated latex photometric immunoassay (LPIA) system with latex reagents conjugated with anti-fibrinogen, anti-fibrinogen-E domain, and anti-neoantigen of fibrinogen-D domain antibody, respectively. Although correlation coefficients between each pair were statistically significant, several samples showed discrepancies among the three FDP parameters. In order to test the possibility that these discrepancies might be caused by the difference in the affinity of the three antibodies to each FDP fragment, we analyzed the profiles of FDP fragments by Western blotting. Consequently, we found abundant high-molecular-weight (HMW) and/or D fragments in some samples which showed low FDP-DD and FDP-E values relative to FDP-T values. Generally, FDP-DD is considered to be the most reliable parameter for monitoring fibrinolysis. However, our study indicated that FDP-T is remarkably useful whenever FDP-DD values are lower than expected from the clinical parameters and/or other laboratory test data used for diagnosis of DIC.View full abstractDownload PDF (3870K) -
Tohru Higashino, Kunio Kobayashi, Kazumasa Hirauchi, Kiyohisa Uchida1995 Volume 24 Issue 1 Pages 32-35
Published: March 31, 1995
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSAn immunological pretreatment method using a microplate was developed for fractional determination of low concentrations of α-fetoprotein (AFP) in serum. This method allows simultaneous partial purification and concentration of AFP from many serum samples. The pretreated sample can then be subjected to lectin-affinity electrophoresis (LAE) coupled with detection by antibody-affinity blotting. More than 60% of the AFP was recovered by the partial purification from serum containing less than 100μg/l of AFP, and the specific activities for the total protein was raised more than 20-fold. There was no bias or shift in AFP composition between the untreated and partially purified samples. By the present pretreatment method, AFP at a concentration of as low as 10μg/l in serum was concentrated and detected by the LAE coupled with the antibody-affinity blotting.View full abstractDownload PDF (2051K)
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