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Volume 26, Issue 3
Displaying 1-6 of 6 articles from this issue
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Toyofumi Nakanishi, Masahiko Kishikawa, Ayako Miyazaki, Akira Shimizu1997 Volume 26 Issue 3 Pages 115-124
Published: September 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSProgress in clinicopathological research for hemoglobinopathies and neurodegenerative diseases have increased the need for simple and definite methods to detect and characterize mutant proteins, e. g., amyloid protein, Cu/Znbinding superoxide dismutase (SOD-1), prion protein accociated with the diseases. The DNA analysis is a simpler current method for detection of mutations, however, it does not elucidate the post-genome informations such as the posttranslational modification, protein-protein or -DNA interactions and the ratio of wild to mutant proteins, which are not available directly from the genome. The mass spectrometry (MS) may offer a powerful tool for these postgenome analysis. In 1994, we have established immunoprecipitation as a simple and reliable procedure for detecting mutant protein by MALDI-TOFMS and by HPLC-ESIMS. By this procedure, the isolation, concentration and desalting of the mutant proteins to be tested could be achieved simultaneously. Herein, we applied this technique for the detection of mutant proteins associated with diseases such as familial amyotrophic lateral sclerosis, familial amyloid polyneuropathy, hemoglobinopathies and characterized some mutant proteins included new mutations by MS.View full abstractDownload PDF (1040K) -
Yoshihiko Maehara, Shinya Oda, Eiji Oki, Eriko Tokunaga, Keiza Sugimac ...1997 Volume 26 Issue 3 Pages 125-133
Published: September 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSA number of laboratory tests have been used to diagnose cancer and for post-treatment follow-up of patients with cancer. The aims of these tests are; 1, evaluation of the biological characteristics of each cancer; 2, identification of micrometastases of cancer, for example to the lymph node, bone marrow and peripheral blood; 3, detection of patients at high risk for developing cancer. Cancer diagnosis and treatment should be improved by further develop- ments in molecular biology and clinical chemistry, in the next century.View full abstractDownload PDF (5065K) -
Koji Shishino, Shuichi Saheki, Nozomu Takeuchi1997 Volume 26 Issue 3 Pages 134-142
Published: September 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSLDL subclasses were determined by nondenaturing polyacrylamide slab gel electrophoresis in sera from normal and hyperlipidemic subjects (IIa, IIb and IV) and their size distributions were examined with reference to the levels of apolipoproteins. Apolipoproteins B, C-II, C-III and E were significantly high in sera from hyperlipidemic patients, while there was no significant difference in the level of apolipoprotein A-I. The smaller LDL subclasses (Rf = 0.80, 0.84, 0.92) appeared in hyperlipidemic patients but not in normal subjects. Smaller LDL subclasses showed increased prevalence among patients with hyperapolipoproteinemia and tended to be increased among patients with vascular complications; cerebral infarction, myocardial infarction, hypertension and diabetes mellitus with complications.View full abstractDownload PDF (1155K) -
Hiroya Hidaka, Minoru Tozuka, Kazuyoshi Yamauchi, Tetsuo Nakabayashi, ...1997 Volume 26 Issue 3 Pages 143-149
Published: September 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSThe direct method and the precipitation-based method of high density lipoprotein-cholesterol (HDL-C) assay demonstrated different HDL-C levels for two serum samples, one with a high level of apolipoprotein E (apoE) and one containing slow-alpha lipoprotein. We compared HDL-cholesterol reaction for three serum samples with a high apoE level, slow-alpha lipoprotein, and normal sera using the direct, precipitation and gel filtration chromatography methods. There were three patterns detected in the samples: (1) Samples showing correlation on all three methods,(2) Samples showing correlation between the direct and the precipitation method, but no correlation between the direct method and gel filtration chromatography, and (3) Samples showing no correlation among the three methods. These results suggests that abnormal HDL with large particle size includes three or more kinds of apoE-rich HDL or slow-alfa lipoproteins with different origins or hysiological and chemical characteristics.View full abstractDownload PDF (713K) -
Kazuyoshi Yamauchi, Minoru Tozuka, Hiroya Hidaka, Tetsuo Nakabayashi, ...1997 Volume 26 Issue 3 Pages 150-156
Published: September 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe evaluated the lipoprotein specificity of two direct methods based on different principles for quantifying high-density lipoprotein cholesterol (HDL-C). Reagent a, utilizing polyethlene glycol-modified enzymes and sulfated α-cyclodextrin, showed about 6% crossreactivity for very low-density lipoprotein (VLDL), while reagent b which consisted of polyanion and dispersive surfactant showed about 5% crossreactivity for low-density lipoprotein (LDL). There was difference in the reactivity for HDL3 among the two direct methods and the precipitation method, but both direct methods exhibited a higher cholesterol value for HDL2 than the precipitation method. To investigate the reactivity to HDL2 in detail, the HDL2 fraction was separated into HDL with apo E and HDL without apo E by heparin-sepharose affinity chromatography. The precipitation method measured only HDL without apo E, but HDL-C measured by the two direct methods included both of HDL with and without apo E. HDL-C values by the direct method were in agreement with the values of total cholesterol in HDL fractions isolated by ultracentrifugation.View full abstractDownload PDF (2158K) -
Study of Exclusion Criteria for Reference Intervals of Cholesterol, Triglyceride and HDL-cholesterolHiroko Ohno, Eiko Jimbo, Yoshikazu Aoki1997 Volume 26 Issue 3 Pages 157-161
Published: September 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe studied exclusion criteria to determine the reference intervals for cholesterol (TCHO), triglyceride (TG), and HDL-cholesterol (HDLC) according to the NCCLS-proposed guideline. Significance of differences on lipid tests was examined between healthy subjects and subjects with few symptoms. Ranking of the usefulness of 35 laboratory tests on TCHO, TG, HDLC tests was calculated using multiple regression analysis in a single operation. Hypertension, hyperlipemia, hyperuricemia, diabetes, and liver diseases were found to affect the data of TCHO, TG and HDLC, respectively. Data for the lipid tests were also highly correlated with the those of lactate dehydrogenase, cholinesterase, uric acid, glucose and body mass index which reflect the nutritional conditions. The reference intervals obtained from this study were wider than the therapeutic management decision levels recommended by the Japan atherosclerosis society.View full abstractDownload PDF (610K)
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