In order to clarify a motile specificnty of lymphocytes, a contactile protein was extracted from human tonsilar lymphocytes. The purified protein exhibited superprecipitation at low ionic strength in the prerence of Mg⁺⁺and Ca⁺⁺, Mg⁺⁺acivated ATPase activity (EC 3.6. 13.), ATP sensitive viscosity fall and the typical“arrowhead structure” characteristic of myosin B of skeletal muscles electronmicrorcopically. These findings were similar to those of the contractile protein obtained from neutrophils which have reported previously by us except a few points. Judging from the attitudes to Mg⁺⁺ and Ca⁺⁺, the protein is a Ca⁺⁺ sensitive contractile protein, so-called a natural actomyosin, and a smooth murcle type.
The protein was localized in the whole cytoplasma, contrary to that the contractile protein of neutrophils was located only just out of the granular layer, and its quantity was more than neutrophils. An arrangement of the protein was obviously different from that in neutrophils. Studying the change of the contractile protein on the course of cell culture by using FlTC-heavy meromyosin, the fluorescence was also observed in PHA-induced immature lymphocytes that had no motile activity. On the basis of these results, a role of the contractile protein in lymphocytes was discussed.

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There have been many diagnostic methods for liver diseases by determining various enzymes released into the serum. However, the enzymatic methods using liverspecific enzymes as a paramater of liver injury were scarcely reported. Ornithine carbamyltransferase (OCT) which is mainly located in hepatic parenchymal cells, was measured in serum of various hepatic diseases and metabolic fatty liverrats preparing with treatments of ethionin, orotic acid or vitmin B₆-deficiency-high protein diet.
OCT activity in serum was increased only in the patients of hepatic diseases and injuries. To be emphasized, the enzyme levels in serum were specifically elevated in these fatty livers, whereas GTP, GOT and γ-glutamyl transpeptidase in these serum were not increased.
Since there has been no satisfactory methods to determine the degree of fatty liver, the assay of OCT in serum is expected to be a useful tool for diagnosing the patients with fatty liver.

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The preparation and antigenic properties of dehydroepiandrosterone sulphatebovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through the C-17 position are described. Antibody raised against this antigen in the rabbit posses sed a high affinity (Ka=1.43×10⁹M^-1) and excellent specificity to dehydroepiandrosterone 3-sulphate, exhibiting little or no cross-reactivities for related free and conjugated steroids with exceptions of 5-androstene-3β, 17β-diol 3-sulphate (67.5%) and 16α-hydroxydehydroepiandrosterone 3-sulphate (13.7%).

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The Reitman-Frankel method has been widely used as the laboratory assay method for serum transaminase activities in Japan, and the author deals with outlines of its reaction mechanism and procedure. Furthermore, several problems to be criticized were also pointed out.
This method has many disadvantages more than advantages, and the essential solution of these problems might be so far away, inspite of many efforts have been carried out. We know also that usuages of this method is not so frequent unlike the case in Japan.
Therefore, the author hopes to recommend to use the rate assay under the suitable conditions in order to assay the activities of serum GOT and GPT. Our efforts and cooperations for this purpose should be appreciated.

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The isoenzymes of aspartate aminotransferase, m-AST, s-AST were purified from human liver. The assay conditions of AST activity were investigated by use of the purified AST isoenzymes and patient's sera with respect to substrate and pyridoxal 5'-phosphate (PALP).
The affinities of m- and s-AST for aspartate were remarkedly differed, Km values were 0.5mM and 3.1mM, respectively. Over 100mM of aspartate, m-AST activity was inhibited, whereas s-AST was none.
The activation of AST activity by PALP were observed in all these specimens. The preincubation of specimen with PALP alone showed much more rapid activation than with PALP plus aspartate. The inhibitory action of aspartate to PALP binding was excluded by the short preincubation with PALP and specimen.

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Low values for glutamic oxalacetic transaminase (GOT) were observed in the serum of uremic patients. After in vitro dialysis of the serum or with addition of pyridoxal phosphate to the assay medium, however, serum GOT activity of the patients was increased and remarkable increased activity was observed after in-vitro dialysis and with addition of pyridoxal phosphate to assay medium.
These findings suggested not only that the dializable inhibitors of GOT appeared in uremic serum but also that conversion of apoenzymes to holoenzyms was disturbed in uremic serum on account of deoiciency of pyridoxal phosphate due to long term hemodialysis.

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