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Volume 26, Issue 2
Displaying 1-9 of 9 articles from this issue
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Tomotaka Shinoda, Takashi Isobe, Muhammad Alim, Satosi Yamaki1997 Volume 26 Issue 2 Pages 52-61
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
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Toshiyuki Yamada1997 Volume 26 Issue 2 Pages 62-68
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
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Fumitake Gejyo1997 Volume 26 Issue 2 Pages 69-73
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
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Tomoyuki Uemichi1997 Volume 26 Issue 2 Pages 74-81
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
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Kunio Yagi1997 Volume 26 Issue 2 Pages 82-88
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSFor the determination of lipid hydroperoxides, we previously reported a method that involved the reaction with 10-(N-methylcarbamoyl)-3, 7-(dimethylamino)-phenothiazine under catalysis of hemoglobin and measurement of its product methylene blue. In an attempt to apply this method directly to serum, we found that dissociation of lipid hydroperoxides from serum proteins can be achieved by lipoprotein lipase, and the interfering substances, holotransferrin and ascorbic acid, can be eliminated by adding triethylenetetraminehexaacetic acid and ascorbate oxidase. Pretreatment with these ingredients enabled us to perform a simple assay of serum lipid hydroperoxides.View full abstractDownload PDF (787K) -
Yoshiko Usami, Kazuhiro Iguchi, Tetsuo Adachi, Hajime Yamamoto, Kiyosh ...1997 Volume 26 Issue 2 Pages 89-94
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSSpecimens of urine were obtained before and after digital rectal examination from four patients with benign prostatic hypertrophy (BPH) for comparative analysis of protein components by reversed-phase high performance liquid chromatography. Four characteristic peaks were detected in urine after compared with before the physical examination, and their molecular masses on SDS-polyacrylamide gel electrophoresis were 16, 16, 34 and 46 kDa. After the proteins were reduced, S-pyridylethylated and cleaved with cyanogen bromide, the amino-terminal amino acids were sequenced and a homology search was conducted. The two 16-kDa proteins were both identified as β-microseminoprotein. The 34-kDa protein was identified as a prostate-specific antigen, and the 46-kDa protein was a Zn-α2-glycoprotein. The present findings provide important information on pre-analytical sampling of urine for the diagnosis of BPH.View full abstractDownload PDF (1914K) -
Kenichi Adachi, Kenichi Kakigawa, Yasuhiko Tazuke, Yoji Tsukada1997 Volume 26 Issue 2 Pages 95-100
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
JOURNAL FREE ACCESSWe have developed a simple automatic method for measuring sulfated bile acids in urine using an enzymatic method. The procedure involves sequential use of enzymes, bile acid sulfate sulfatase, β-hydroxysteroid dehydrogenase and 3-oxo-5β-steroid Δ4-dehydrogenase, followed by reductive color-generating reactions with the hydrogen derived from two dehydrogenase reactions. The present method is characterized by the use of water-soluble tetrazolium WST-1 as a chromogen. The formazan of the reducing product of WST-1 has high water solubility and does not pollute the cuvette or analyzer tube. The calibration curve for glycolithocholic acid 3-sulfate (GLCA-S) as the standard was linear up to 200μmol/l. The CVS for the within- and between-run precisions were 0.5-1.2% and 2.3-2.7%, respectively. Recovery of various sulfated bile acids in urine averaged 100.1%. A good correlation (r=0.995, n=52) was obtained between this automatic method and the manual method previously developed.View full abstractDownload PDF (639K) -
1997 Volume 26 Issue 2 Pages 101-105
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
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1997 Volume 26 Issue 2 Pages 106-114
Published: June 30, 1997
Released on J-STAGE: November 27, 2012
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