Japanese Journal of Clinical Chemistry

Japanese Journal of Clinical Chemistry

Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633

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Volume 34, Issue 3

Displaying 1-6 of 6 articles from this issue

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In vitro selection of functional nucleic acid molecules

Shoji Ohuchi, Yoshikazu Nakamura

2005 Volume 34 Issue 3 Pages 197-204
Published: September 15, 2005
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.34.3_197

JOURNAL FREE ACCESS

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World of Antisense RNA

Tadao Funato, Mayu Takeda, Minako Takahashi

2005 Volume 34 Issue 3 Pages 205-215
Published: September 15, 2005
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.34.3_205

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Functional analysis with RNA interference (RNAi)

Kayoko Yamada, Sayumi Shibamoto, Takayuki Mizutani

2005 Volume 34 Issue 3 Pages 216-223
Published: September 15, 2005
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.34.3_216

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Novel and artificial RNAs

Takeshi Imanishi, Satoshi Obika

2005 Volume 34 Issue 3 Pages 224-232
Published: September 15, 2005
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.34.3_224

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RNAi and its potential for the application of nucleic acid drugs

Jun Yasuda

2005 Volume 34 Issue 3 Pages 233-239
Published: September 15, 2005
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.34.3_233

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Development and Characterization of Small, Dense LDL Cholesterol Assay Based on Filter Method

Yasuki Ito, Tsutomu Hirano, Gen Yoshino

2005 Volume 34 Issue 3 Pages 240-247
Published: September 15, 2005
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.34.3_240

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Small, dense LDL has most atherogenic property among various subclasses of LDL. We developed a new and simple analytical method for quantification of small, dense LDL cholesterol, utilizing a separating agent with polyanion and divalent cation natures. As the first assay step, large buoyant LDL, and other apoB-containing lipoproteins (d<1.044g/mL) are aggregated with the separating agent, and are removed through a membrane filter. As the second step, small dense LDL cholesterol obtained is measured enzymatically on a common analyzer. The process enables to actually measure the target analyte even in chylomicron rich samples.
The analytical evaluation studies showed good preci-sion and linearity without any disturbance owing to representative endogenous substances. The comparison studies with other methods indicated good correlations with ultracentrifugation, electrophoresis, NMR and HPLC methods. The best correlation was found with the ultracentrifugation method (r=0.927). The proposed method is easy to perform on a common analyzer, and can be used widely as a routine laboratory method.

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