A gaschromatographic determination and colorimetric measurements by Zimmermann's reaction for urinary 17-ketosteroids were compared. For the latter fraction by stepwise elution column chromatography on alumina and total urinary 17-ketosteroids by general method had been measured.
Gaschromatographic analyses were carried out on 3% XE-60 at programmed temperature 180-210°.
In the colorimetric methods the value of total urinary 17-ketosteroids was indefinite, and the value of dehydro-epiandrosterone fraction by column chromatography was higher and that for 11-oxy-17-ketosteroids was lower.
11-deoxy-17-ketosteroids, 11-keto-17-ketosteroids, and 11-hydroxy-17-ketosteroids have a characteristic absorption at 520mμ, and correction is necessary when dehydroepiandrosterone is used as standard.

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It was reported that glucose showed various effects on the butyrate metabolism in sheep liver slices and these effects were changed by alloxan treatment. Presently a report was made of influences of glucose metabolites upon the butyrate metabolism in liver slices of normal, starved and, alloxan diabetic sheep.
The experimental results were summarized as follows: 1) In normal and starved sheep, the ¹⁴C transfer from ¹⁴C-butyrate into glucose and NEFA was decreased by the addition of phosphoenolpyruvate and the formaion of ¹⁴C-cholesterol, -triglycerides and-phospholipid from ¹⁴C-butyrate was increased by the addition of phosphoenolpyruvate. These effects of phos phoenolpyruvate on the butyrate metabolism disappeared following treatment with alloxan. 2) In starvation the ¹⁴CO₂ formation from ¹⁴C-butyrate was increased by the addtion of phosphoenolpyruvate. But there were no effects of phosphoenolpyruvate addition on the formation of ¹⁴CO₂ from ¹⁴C-butyrate in normal and alloxan diabetes sheep. 3) Normal, starved, and alloxan diabetic sheep, showed no effects of phosphoenolpyruvate addition on the ¹⁴C transfer from ¹⁴C-butyrate into ketone bodies. 4) In normal, starved and alloxan diabetic sheep, the addition of pyruvate and lactate did not influence the ¹⁴C transfer from ¹⁴Cbutyrate into CO2, glucose, ketone bodies and all lipid fractions.

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In detecting Australia (Au) antigen in the serum evaluation was made of the use of rheophoresis which increases sensitivity by concentrating reagents through tne hydrodynamic force produced by local evaporation, and shortening the time required for reaction. The results obtained were as follows.
1. In rheophoresis, the diffusion of samples was directed to the center, while it was uniformly in all directions from the wells in Ouchterlony's agar gel diffusion.
2. The sensitivity of saveral methods was compared by the detectability of the various concentrations of Au antigen-containing serum. Au antigen was detectable up to 1:128 dilution in rheophoresis and the sensitivity was almost equal to that of immunoelectrosyneresis.
3. The sensitivity of rheophoresis for the detection of Au antigen-antibody complex was almost equal to that of immunoelectrosyneresis, but Au antigen was not detected in latex agglutination.
4. The usefulness of rheophoresis for the detection of Au antigen was discussed.

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A coloring matter produced in tne reaction between oxalacetic acid and Fast Violet B Salt (6-benzamido-4-methoxy-m-toluidine diazonium chloride) in almost a natural medium was isolated as red-violet needles of a melting point of 198°. An attempt was made to confirm whether the structure of the coloring matter produced and used fcr the determination of GOT activity was formazane as already postulated. And the compound was identified as the red form of a formazane derivative (Chart 1) on the basis of its spectral properties.

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