Japanese Journal of Clinical Chemistry

The Current Topics of Laboratory Examination Methods for the Disturbaces of Lipid Metabolism and their Clinical Consideration

IWAO FUKUI

1980 Volume 9 Issue 1 Pages 1-11
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_1

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HMG-Coenzyme A Reductase, Methods of Assay and Regulation of Activity

YOSHIFUMI IWASAKI

1980 Volume 9 Issue 1 Pages 12-20
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_12

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Determination Methods of Cholesterol 7α-hydroxylase Activities and their Significances

NOZOMU TAKEUCHI

1980 Volume 9 Issue 1 Pages 21-29
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_21

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Physiological Role of Hepatic TriglycerideLipase: Assay Method for Selective Measurement of Hepatic Triglyceride Lipase and its Regulation by Insulin

TSUGUHIKO NAKAI

1980 Volume 9 Issue 1 Pages 30-43
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_30

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Plasma Triglyceride and its Hydrolyzing Enzyme System

TOSHIO MURASE, NOBUHIRO YAMADA

1980 Volume 9 Issue 1 Pages 44-49
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_44

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Quantitative Determination of Serum Apolipoprotein

KYOSUKE YAMAMOTO, SHUNICHI KOGA

1980 Volume 9 Issue 1 Pages 50-58
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_50

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High Density Lipoprotein Cholesterol Determination and its Clinical Significance

TOSHITSUGU ISHIKAWA, HARUO NAKAMURA

1980 Volume 9 Issue 1 Pages 59-67
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_59

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Studies on Chemical Composition of the Aorta in Relation to Atherosclerosis

TOKUTARO SATO

1980 Volume 9 Issue 1 Pages 68-77
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_68

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Leucocytic Micuosomal Frction as Relaxing Factor

YUKO WADA, NORIYUKI TATSUMI, KIYOSHI OKUDA

1980 Volume 9 Issue 1 Pages 78-83
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_78

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To understand the relaxation mechanism in blood cells, the microsomal fracton was prepared from leucocytes. In this leucocytic microsomal fracton (LMF), ATP-dependent and oxalate-enhanced Ca²⁺ uptake could be observed, and when LMF was added to the superprecipitation reaction system of leucocyte myosin B in the absence of EGTA, the superprecipitation was remarkably depressed. Evidences, showing that salyrgan inhibited Ca²⁺ uptake and ATPase activity of LMF whereas sodium azide did not exhibit any inhibitory effect on both functions, suggested that no mitochondrial fraction was contaminated in this microsomal fraction from leucocytes.
From our investigations including electron microscopic study, it was proved that a leucocyte had the microsomal fraction as a relaxing factor and its nature resembled the sarcoplasmic relaxing factor from the smooth muscle or the cardiac muscle.

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Normal Range Calculation:Manual Puocessing of the Data which Conlain Abnoumal Data

TOSHIAKI USUI

1980 Volume 9 Issue 1 Pages 84-96
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_84

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A manual calculation method of the normal range from the data which contain a certain number of abnormal data was presented. The data are summarized in a frequency table, and the classes with the frequency less than one fifth of the maximum frequency are eliminated (initial truncation). Cumulative sum of the remaining classes is calculated (truncated cumulative sum), and 24.4 percentile (X₂₄) and 75.6 percentile (X₇₆) arecalculated from the truncated cumuiative sum. The lower limit (R_L) and the upper limit R_Uof the normal range are calculated as follows: d=x₇₆-x₂₄, R_L=x₂₄-d, R_U=x₇₆+d. Classes with the class mark less than RL and those with the class mark larger than RU are eliminated from the original frequency table, and the remaining classes are subjected to repeated calculations as described above, until R_L and R_U give constant values. This method was extended to the log-normal distribution processing without log calculation. The only difference from the normal distribution processing as described above is R_L and R_U calculation, which are calculated as follows: r=x₇₆/x₂₄, RL=x₂₄/r, R_U=x₇₆ r. The calculated range was less affected by contamination of abnormal data up to 20 per cent.

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Effect of Dilazep on the Automaticity of Rat Isolated Heart

TORU YOKOYAMA, SETSURO FUJII

1980 Volume 9 Issue 1 Pages 97-103
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_97

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The effect of Dilazep (1, 4-bis [3, 4, 5-trimethoxybenzoyloxy) propyl] perhydro-1, 4-diazepine dihydrochloride monohydrate)on the automaticity of rat isolated heart in erythrocyte suspension and whole blood was studied. The effect of Dilazep was compared with Dipyridamole, Propranolol, o-lodo Sodium Benzoate (OISB) and the metabolites of Dilazep (Monoester-type, Trimethoxybenzoic acid and bis-Hydroxypropyl perhydro-1, 4-diazepine). Although Dilazep prolonged the automaticity with the concentration more than 3×10^-6M in vitro, or after 0.3mg/kg i.v. or 300mg/kg p.o. administration, Dipyridamole, Propranolol, and OISB showed no prolongation. On the contrary, Propranolol and OISB suppressed the automaticity in high concentration. Among the metaboiltes of Dilazep, only monoester-type prolonged the automaticity, and it's intensity corresponded to about 1/100 as that of Dilazep.
Although it has not been clear the action mechanism of Dilazep on the automaticity, it is considered Dilazep would act through erythrocyte, because it's effect was seen in whole blood or erythrocyte suspension.

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HPIC Analysis of Bile Acids Using a New Techhique of Post Column Reaction

KAZUTAKA ARISUE, ZENSUKE OGAWA, KAZUMA KOHDA, CHOZO HAYASHI, YASUO ISH ...

1980 Volume 9 Issue 1 Pages 104-110
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_104

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Many trials have been made with HPLC, on bile acids determination,and introduced, the enzymatic method uslng immobilized 3α-hydroxysteroid dehydrogenase (3α-HSD) as an effective method to detect of bile acidsand its conlugates, for post column reac-tion technique,in this paper.
DuPont Zorbax C₈ were used as the LC column which was housed in column oven to keep at 55°, and used the alcohol-phosphate buffer mixture as the mobile phase.
The enzyme were immobilized on aminated porous glass beads with glutaraldehyde, and packed in stainless steel column. The reaction reagent contaming NAD and DTT (dithio threitol) in phosphate buffer was mixied just before the immobilied enzyme column.
The fluorescence intensity of the NADH thus produced were measured, in the presence of bile acids as substrate.
Some results are described on some conditions for separation and determination in this paper.

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Screening Test for 21-hydroxylase Deficiency

YOSHIHISA YAMAGUCHI, CHOZO HAYASHI, JUN-ICH IWAMURA, YOSHIHARU KOBAYAS ...

1980 Volume 9 Issue 1 Pages 111-116
Published: April 25, 1980
Released on J-STAGE: November 27, 2012

DOIhttps://doi.org/10.14921/jscc1971b.9.1_111

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We have determined urinary 5-pregnene-3β, 17α, 20α-triol and dehydroepian-drosterone for screening test of 21-hydroxylase deficiency or low actlvity using gas-liquid chromatographic method.
Excretion value of urinary pregnenetriol was extremely high with patients of adrenal tumor and fairly high with 21-hydroxylase deficiency and Cushing's syndrome compared with normal sublects, but the value of dehydroepiandrosterone/pregnenetriol ratio was 4.32 for adrenal tumor, 2,03 for Cushing's syndrome, 0.08 for 21-hydroxylase deficiency and 3.71 for normal adult.
Ratio of dehydroepiandrosterone/pregnenetriol was significantly low so that the assay of these compound could be used as a diagnostic tool for screening test of 21-hydroxylase deficiency same as that of pregnanetriol assay.

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