The present study aimed to clarify how mothers who accompany children aged less than 2 years blood drawing procedure feel about their experience. Mothers of 16 children aged less than 2 years underwent semi-structured interviews. Analysis of the results using the Modified Grounded Theory Approach (M-GTA) led to the development of 10 categories and 28 concepts. Some mothers accompanying their children felt "anxious regarding separation due to past experience" and other mothers felt "obvious relief to be able to accompany her child." Mothers who felt that they "wanted to provide a sense of security to their child by being there" or who had "decided to accept their child's reaction" were able to anticipate and make judgments regarding their child's actions. Meanwhile, those who were "anxious because of not understanding their child's experience" and "troubled by watching their child's reaction" tended to accept information in a negative manner. The above results suggested that support to help mothers engage with their child in a calm manner was required to reduce the anxiety of mothers.