Velocities of elementary steps and two-dimensional nucleation rates on the {101} face of glucose isomerase (GI) crystals were measured at pressures of 0.1, 25 and 50 MPa. We conducted
in situ observation of the steps by using a laser confocal microscope combined with differential interference contrast microscope (LCM-DIM) and high-pressure vessel with a sapphire window (t = 1 mm). Both step velocities and 2D nucleation rates increased with increasing pressure at
T = 26.4°C and
C = 5.6 mg ml
-1. Solubilities C
e at high pressures were also measured by
in situ observation of the moving steps around equilibrium temperatures. The solubility decreased with increasing pressure.
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