高圧力の科学と技術
Online ISSN : 1348-1940
Print ISSN : 0917-639X
ISSN-L : 0917-639X
最新号
選択された号の論文の11件中1~11を表示しています
巻頭言
特集:分子集合体の機能制御と創成を目指した高圧力生命科学
  • 阿部 文快
    2021 年 31 巻 2 号 p. 54-65
    発行日: 2021年
    公開日: 2021/10/27
    ジャーナル フリー

    High hydrostatic pressure in the range of several dozen MPa, are generally assumed to be nonlethal but exert adverse impacts on growth of organisms that are adapted to atmospheric pressure. Deep-sea organisms have established intracellular mechanisms to cope with such extreme environments. A living cell is composed of myriad molecules including nucleic acids, proteins, lipids, ions, and various low molecular compounds. These molecules interact with each other transiently or statically, eventually eliciting innumerable intermolecular interactions even in a small microbial cell. The complexity hampers the understandings of adaptive mechanisms to high pressure employed by deep-sea organisms. Studies with model organisms such as a bacterium Escherichia coli and a yeast Saccharomyces cerevisiae offer breakthroughs to unravel the effects of high pressure on the complex intermolecular networks in living cells. This review summarizes recent advances in high-pressure biology as well as classic issues in this field, especially focusing on remodeling of intracellular systems to adapted to high-pressure environments.

  • 八木 俊樹, 西山 雅祥
    2021 年 31 巻 2 号 p. 66-73
    発行日: 2021年
    公開日: 2021/10/27
    ジャーナル フリー

    Eukaryotic flagella and cilia generate rhythmical beating motions. When the structure is partially defective, the motility is severely impaired. We have developed a high-pressure microscope that is optimized both for the best image formation and for the stability to hydrostatic pressure up to 150 MPa. Here, we show that high hydrostatic pressure induces vigorous beating motion of Chlamydomonas flagella with defect structures. In addition, our results suggest that application of pressure increased intra-flagella Ca2+ concentration. The present technique could be extended to study other protein machineries and cell dynamics.

  • 木下 智和, 福原 学
    2021 年 31 巻 2 号 p. 74-81
    発行日: 2021年
    公開日: 2021/10/27
    ジャーナル フリー

    Hydrostatic pressure is one of the fundamental and significant external stimuli, and thus, has attracted attention in synthetic chemistry and photo-physical and -chemical processes. In considering weak interaction systems such as biological and supramolecular reactions on the basis of thermodynamics, enthalpy and entropy changes exquisitely keep the balance each other according to the enthalpy-entropy compensation law, meaning that it seems to be difficult to control reactions/rates by only either of these parameters. On the other hand, reaction/activation volume change (ΔV)-based hydorstatic pressure (P) control turns out to be an alternative to the conventional control method. In this review, we wish to report our recent hydrostatic pressure-spectroscopic results on ΔV as solvation, conformational change, molecular recognition, and biological reaction.

  • 千葉 文野, 秋山 良
    2021 年 31 巻 2 号 p. 82-89
    発行日: 2021年
    公開日: 2021/10/27
    ジャーナル フリー

    Recently isotactic poly(4-methyl-1-pentene) (P4MP1) has been found to selectively absorb long-chain alkanes from alkane mixed systems. In this article, we introduce the idea of this study from the pressure-temperature phase diagram. We also show that such selective absorption characteristics can be explained from a simple model, namely Asakura-Oosawa theory, and the relationship with molecular recognition is explained.

  • 李 書潔, 北原 亮
    2021 年 31 巻 2 号 p. 90-95
    発行日: 2021年
    公開日: 2021/10/27
    ジャーナル フリー

    Host-guest interactions between a pair of naphthalene-based molecular tubes (i.e., anti-isomer and syn-isomer) and 1,4-dioxiane have been studied to understand selective recognition. Volume changes for complexation of the amide naphthotubes with 1,4-dioxane were investigated using high-pressure fluorescence and high-pressure NMR spectroscopy. We found that the partial molar volume change (ΔV°) for the association of 1,4-dioxane with the naphthotubes was -6.3±0.1 mL mol-1 for the anti-isomer and 3.2±0.4 mL mol-1 for the syn-isomer. To elucidate the molecular basis of ΔV°, molecular dynamics simulations of the complexation process were also performed. The difference in ΔV° was attributed to variations in the shape and hydration of naphthotube hydrophobic cavities.

受賞記念解説:高圧力学会学会賞
受賞記念解説:高圧力学会奨励賞
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