We attempted to clarify the reason for the great affinity of Mono-L-aspartyl chlorin e6 (NPe6) for albumin by analyzing the sites of interaction of human serum albumin (HSA) with NPe6 using Fourier transform infrared spectrometry (FT-IR). The concentration of HSA was maintained constant while that of NPe6 was altered. To distinguish whether aspartic acid residue of NPe6 and to investigate the sites of interaction of HSA, chlorin e6 and dansylsarcotine was used. The binding constant of HSA and NPe6 was different for NPe6/HSA molecular ratios until 1 and greater than 1. But the results between HSA and Chlorin e6 did not show spectral changes. A reagent including HSA and dansylsarcotine was added to various concentrations of NPe6. These were no spectral changes independent of the concentration of NPe6. The result of analysis of FT-1R suggest that the interaction of HSA with NPe6 is held at binding site II of HSA with aspartic acid residue of NPe6 at a molecular ration of 1: 1.

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A 3-year-old Asian girl with a phacomatosis pigmentovascularis type IIb was seen at the outpatient clinic. The patient was treated with the dye laser (585nm, 6J/cm2, 450μs, 7mm), Q-switched ruby laser (694nm, 7J/cm2, 30ns, 4mm) and Q-switched alexandrite laser (755nm, J7/cm2, 50ns, 3mm) under general anesthesia at the interval of at least 4 months. As the outcome of the treatment was good to excellent, we believe that multiple laser treatment in childhood is an effective therapeutic tool in the treatment of both melanocytic and vascular lesions. We conclude that phacomatosis pigmentovascularis type IIb can be treated successfully by multiple laser treatment in childhood.

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