(1) Using a pure culture of a marine diatom,
Phaeodactylum tricornutum, as an example of phytoplankton, the contents of chlorophyll, RNA, DNA and protein in algal cells were measured, respectively, by the methods of ARNON, OGUR and ROSEN, BURTON and LOWRY
et al.. The results of analyses were compared with those obtained earlier with synchronous cultures of a green alga
Chlorella ellipsoidea.
(2) The plankton harvested from water of an eutrophic Lake Nakanuma (in Ibaraki Prefecture) was subjected to the same methods of analysis. It was found that in the plankton. the RNA/protein ratio was roughly of the same order of magnitude as that for
P. tricornutum. In contrast, the DNA/protein ratio was very high and the chlorophyll/protein, chlorophyll/RNA and RNA/DNA ratios were markedly lower as compared with those obtained for the pure culture of the marine diatom.
(3) Attempt was made at simplifying the procedures of analysis by subjecting the cells of the marine diatom collected, in different quantities, on the Millipore filters to direct spectrophotometric analysis of chlorophyll followed by the RNA analysis using a modified Ogur-Rosen method. An approximately linear relationship was obtained between the quantities of cells and RNA, whereas the corresponding plot made for chlorophyll vs cell quantity was found to consist of two straight lines.
(4) Discussions were made on the significance and suitability of measuring the quantities of chlorophyll, RNA, DNA and protein for the assessment of the quantity of plankton in natural waters.
View full abstract