The ability to degrade
n-paraffin mixture of two bacterial strains,
Caulobacter sp.and
Flavobacterium sp., isolated from sea water of Tokyo Bay was studied experimentallyin the enriched seawater (ESW) medium. These bacteria degraded actively the mixture of
n-tridecane,
n-tetradecane,
n-pentadecane and
n-hexadecane.
The maximum rate of degradation was observed after a lag period of 2 to 8 day andthese bacteria were found to degrade the
n-paraffin mixture at rates calculated to be in arange from 3.3×10
-12 to 3.4×10
-11 mg-oil cell
-1 h
-1 at 20°C.The maximum degradation rate,
rm mg-oil 1
-1 was correlated with the amount of theinitial total
n-paraffin,
S mg-oill
-1, as expressed by the following equation:
rm=(
rm) max (
S/
S+
Km)
where (
rm) max denotes the largest value of
rm when
n-paraffin exists in large excess and Kmis a constant and represents the amount of
n-paraffin at which the degradation rate,
rm, reaches 1/2 of its largest value, (
rm) max.
The values of (
rm) max and
Km were calculated to be as follows: In the case of
Caulobacter sp.(strain KM-1), (nit) max=6.0 mg-oil 1
-1 h
-1 and
Km=191 mg-oil 1Esw-1; in the caseof
Flavobacterium sp., (
rm) max=5.47 mg-oil 1
-1 h
-1 and
Km=152 mg-oil 1Esw
-1.
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