1. Experimentation on Human Body; Especially the Identification of Antigen and Antibody in the Bathers. 1) Clinical Observation of the Bathing Dermatitis in Tamagawa Hot Spring. The incidence of dermatitis is influenced by the frequency of bathing. In case of bathing 2 to 3 times a day, it appears between the 5th and 10th day, and in case of 4 to 7 times, it appears between the 1st and 5th day. It intensifies within a week and declines afterwards. It is liable to appear at the front, upper chest, neck and nucha, also at the private parts and the armpit where it is easy to form erosion. Redding is seen in all cases and papules also in many cases, but almost no feeling of itch is complained of subjectively, but merely the feeling of smart in case of bathing. According to Toriyama of the Department of Dermatology, Tohoka University, it is characteristic that these are almost no changes in the epidermis, but remarkable changes in the papillary and subpapillary layers, such as the extension of blood vessels filled with blood corpuscles, perivascular edema and cell infiltration, mostly round cell infiltration, and a considerable number of polynuclear, nentrophiles, the edema of connective tissue which appears foremost between fibers with the low stainahility of the nucleus of many fibrocytes that seem to be edematous, and to contain some histiocytes. Moreover he presumes that the stimulating substance, probably hydrochloric acid, penetrates to the corium through the sebaceous duct or sudoriferous duct and produces the main changes in the papillary and subpapillary layers causing this peculiar dermatitis. 2) Identification of Mb Antibody in the Serum of Bathers affected with Bathing Dermatitis in Tamagawa Hot Spring. In carrying out the precipitation on the serum of bathers affected with the bathing dermatitis, using the Mb solution of human heart as the antigen, Mb antibody with values of 20 times 2 to 100 times 8 in the bathers with marked dermatitis, that with 20 times 1 to 50 times 1 value in bathers with slight dermatitis, and that of negative value in the control people who did not take a bath, was noticed. Moreover the Hb anitibody was completely negative in bathers with dermatitis. Thus it seemed that the denaturalized Mb is produced at the parts affected with bathing dermatitis in Tainagawa Hot Spring and activates the ground of antibody production to generate the Mb antibody after it is absorbed. 3) Relationship between the Manifestations of Bathing Dermatitis in Tamagawa Hot Spring and the Mb Antibody in the Serum of Bathers. For example, after bathing 2 to 4 times a day the dermatitis broke out at the 4th day, the Mb antibody appeared on the next day; during 8 days with such bathing this antibody was positive; the dermatitis was present till the 8th day after suspension of bathing; the Mb antibody was still positive on the 4th day after the disappearance of dermatitis, but became negative on the 10th day. Thus the production of Mb antibody was observed shortly after the appearance of dermatitis, and the autoantibody disappeared during a period of one to two weeks after the subsidence of dermatitis. 4) Relathionship between the Course of Bathing Dermatitis and the Precipitation between the Self-Urine and-Serum. It is easy to presume that in base of the outbreak of dermatitis the denatruralized tissue substance at that locality might appear in the urine after it is absorbed, Assuming the urine as the antigen to attempt its precipitation with serum, the value turned positive in the reaction between the urine of the 2nd day after the outbreak of dermatitis and the serum of the next day, stayed positive till the reaction between the urine of the 4th day after the subsidence of dermatitis and the serum of the next day, and then turned negative from thereon. Thus it is recognized that the denaturalized tissue substance produced at the locality of dermatitis is excreted into the blood stream and subsequ
This is a summary of a series of experiments we have carried out and are carrying out at Narugo Spa on the effects of hot spring on the functions of digestive organs. Particular attention has been paid to the functions of the stomach and the liver, and studies have been made of the fundamental factors in individual persons which would give rise to the changes in these functions. In looking over the results obtained from our experiments, what draws our attention is the fact that the reaction to hot spring will be different with each individual, no matter how strictly uniform the conditions under which hot spring stimulations were given, that is, each individual does not always respond with the same kind of reaction to the same degree of hot spring stimulation. This is true not only of the results we obtained, but also of the results obtained by our predecessors in various countries. For this reason, it is not seldom that we find in literature conclusions contradictory to one another. Even in the case of the action of hot spring on the functions of the digestive organs, we are obliged to discover some important factors in individuals on whom the action of hot spring works. The question is still unsolved as to what the factors in individuals responsive to the action are, but it has been made clear by our experiments as well as by various experiments performed by our predecessors that they are closely associated with the function of the autonomic nervous system. As to the probable relation between the hot spring bathing and the process of changes in the functions of the autonomic nervous system, there have been discussions either for or against the Hoff's, theory and from our experiment it does not necessary follow that each indibidual responds uniformly in accordance with the process suggested by Hoff. our experiments show that in the early stage of the hot spring bathing the autonomic nervous system shows in most cases what may be called unstable state, but with the lapse of time of bathing, each indibidual tends to return to somewhat stable condition on his own accord. It may be understandable from facts mentioned above when we say that nothing definite can be found in the changes caused by single bathing, and that something like a definite effect can be observed only by successive bathing or so-called “bathing cure”. In short, the foundation of the changes in the functions of various digestive organs caused by successive hot spring bathing is probably the changes in the autonomic nervous system in each indibidual, but as to the process of its changes, it is not necessary to follow Hoff's theory. It is quite natural that there should be considerable difference in the process of changes in the autonomic nervous system between each indibidual because of the difference in the sensibilities of each indibidual to the hot spring, but what is most important is not the process of changes, but the point to which they come. I think a consideration made in this sort of lightd will lead to an easy solution of the effect of hot spring bathing, or so-called bathing cure.