In an attempt to establish maximum permissible concentration of zinc in mineral waters for the internal use as drinking cure, not only toxic effects of zinc but also influence of the external and internal use of a thermal water against zinc poisoning were studied experimentally.
Twenty rabbits, weighing 2kg each, were divided into five groups. The animals in the groups I, II, and III were given 20ml of zinc sulfate solution in distilled water by stomach tube in daily doses of 400mg, 200mg, 80mg, per kg of bodyweight respectively for one month, whereas in the group IV the rabbits were given 20ml of a water of Ito Hot Springs (simple thermals) to which zinc sulfate was added in amount to make up a daily dose of 200mg per kg of bodyweight and pH of the water was adjusted to 2 and 7 respectively.
The group V consisted of control animals to which the same amount of distilled water was given daily with stomach tube also.
Twenty mice weighing 20gm each were divided into 2 groups. Groups I was bathed for two weeks in Ito Hot Springs at 37°-38° once daily for 15 minuites. To group II (control) no bath was given. After two weeks each group was given LD 50 of zinc sulfate solution, 175mg per kg of bodyweight, parenterally.
In group I loss of bodyweight, rise in temperature, increases in blood zinc level and of serum diastase activity, leucopenia, hypoproteinemia, diarrhea, and melena were noticed. Histological investigation revealed necrosis and fatty degeneration of the liver, hyaline droplet degeneration of the kidney, and erosion of the intestine.
In group II loss of bodyweight, rise in temperature, leucocytosis, increase of serum diastase activity, increases in blood zinc level, and edema of the liver were found.
In group III no pathological changes were confirmed.
In group IV, in spite of the same rise in blood zinc level as in group II, neither leucocytosis no increase of serum diastase activity were noted.
Concerning the mortality of the mice there was no marked difference between the bath group and the control group.
It was concluded that the maximum permissible dose of orally given zinc sulfate was between daily 80mg-200mg per kg of bodyweight by administration for a month, namely corresponding to daily 4g in human adult.
As mineral waters with high zinc content in Japan used to be strongly acid and mostly belong to acid vitriol or acid alum vitriol springs, they could not be used as drinking water and even for drinking cure daily only 10-50ml could be taken after dilution with common water. But on an assumption that mineral water taken per os daily is less than one litre, the maximum permissible concentration of zinc sulfate in mineral water is estimated to be 4gm per litre for the internal use during one month.
The highest record of zinc content in Japanese mineral waters is 965mg ZnSO
4 per kg (Sasakura Hot Spring, not issuing at present) and 2.01mg Zn per litre (Arima Hot Springs).
Therefore there would be no probability of zinc poisoning from drinking of mineral water in Japan.
Moreover internal use of a thermal water of Ito inhibited the toxic effect of zinc sulfate even after lowering pH of the water to 2.0.
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