The effects of sulpyrine (56. 2, 100, 178, 320, 562 μg/m
l) on DNA, RNA, and protein synthesis in HeLa S
3 cells at 36.7°C and at high temperature (38.8, 41.1°C) were investigated. The cells at various cell densities (2.0×10
4, 4.0×10
4 and 8.0×10
4 cells/m
l) were inoculated into each well of 24-well plates, and after 24-h cultivation at 37°C, adherent cells were further cultured for 48 h at high temperature with or without sulpyrine. The synthesis of DNA, RNA and protein was inhibited toward high temperature in cases of cultivations without sulpyrine. The synthesis of DNA, RNA, and protein was inhibited in a concentration-dependent manner by the addition of sulpyrine at each temperature. DNA, RNA and protein-IC
50 values (sulpyrine concentration inhibiting synthesis of DNA, RNA or protein by 50% relative to untreated cells) at each temperature showed a tendency to increase with the increase in cell density. DNA and protein-IC
50 values at each cell density at 38.8°C were not significantly lower than those at 36.7°C. However, RNA-IC
50 values at each cell density were significantly (p<0.005) decreased, depending on the temperature. RNA-IC
50 values at a cell density of 4.0×10
4 per m
l at 36.7°C and 38.8°C were decreased from 411.7μ15. 0 μg/m
l to 221.0×23. 3 μg/m
l, respectively. The RNA-1C
50 value at 41.1°C was lower than that at 38.8°C. However, the RNA-IC
50 value at 41.1°C was excluded from the discussion, because DNA, RNA, and protein synthesis was markedly depressed at that temperature. These results suggest that the inhibitory effect of sulpyrine on RNA synthesis in HeLa S
3 cells is enhanced at high temperature.
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