The study evaluated the characteristics of Z. matrella ‘Wakaba’ selected from 250 ecotypes of Zoysia collected in Japan by comparing with commercial cultivars ‘TM9’ (Z. matrella), ‘Emerald’ (Z. japonica×Z. pacifica), and ‘Meyer’ (Z. japonica) using eleven morphological and three turf quality traits. ‘Wakaba’ closely resembles the mor-phology of ‘TM9’ and ‘Emerald’ in terms of leaf width and leaf length, respectively. In addition, ‘Wakaba’ shows high turf quality traits including fast ground covering, later winter dormancy and faster green-up in early spring which is distinct from ‘TM9’, ‘Emerald’ and ‘Meyer’. Our study also demonstrated the effectiveness of microsatellite markers in distinguishing 26 zoysiagrass cultivars including ‘Wakaba’ by providing a genetic fingerprint for each Zoysia cultivar and supportive information about relationships among the cultivars with regard to their species type and hybridity. Furthermore, ‘Wakaba’ can be useful for studying genetic variance and to understand the genetic control of interesting traits, due to its high heterozygosity. We hope that these microsatellite markers will be useful for the clear identifica-tion of zoysiagrass cultivars, and for the protection of breeders’ rights.