Turfgrass has a practical property that it is always cut (short and trimmed). In such conditions, it is difficult to discriminate the difference in phenotype unless there is a distinctive morphological feature. In this study, we used six cool-season cultivars of creeping bentgrass (Agrostis stolonifera L.) and identified them using DNA microsatellite (SSR) markers. We detected SSR markers that can identify six cultivars of creeping bentgrass. Furthermore, the molecular phylogenetic trees created for the six cultivars of creeping bentgrass based on polymorphism information using SSR markers well reflected the breeding lineage of each cultivar.
A survey was conducted on the lawn levee for three years to examine the maintenance and management actions of farming corporation and landowner, such as mowing. The mowing by the farming corporation was carried out two to four times from mid-April to early October, and the canopy height ranged from 12 cm to 21 cm which was almost the same level of our 3 times a year weeding. On the other hand, landowner was conducted weeding 4 to 5 times a year, and the height of the canopy ranged from 9 cm to 13 cm. where there were few large weeds.