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Volume 23 (1994 - 199･･･
- Issue 2 Pages 121-
- Issue 1 Pages 5-
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Volume 23, Issue 2
Displaying 1-9 of 9 articles from this issue
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Fumitaka TANGO, Yasutomo TAKEUSHI, Masaru OGASAWARA, Makoto KONNAI, Te ...1995 Volume 23 Issue 2 Pages 121-127
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSSeedlings of Zoysia japonica Steud were grown in soil. Primary tillerings arised from the nodes at the base of the second and third leaf-sheath at 5 and 6-leaf stage, respectively and the second tillerings arised from the nodes at the base of the first leaf-sheath of the primary tillerings. Main shoots elongated and developed into stolons at 8-10 leaf-stage of the shoots after the development of 4-7 tillerings. Growth pattern of the seedlings grown in a gravel culture was the same as the pattern of the seedlings grown in soil.
The seedlings at 3-leaf stage were grown in gravel culture containing nutrients and 0.001-01 ppm plant growth ragulators for 7 weeks in a greenhouse. AVG, inhibitor of ehylen-biosynthesis, promoted the increase of the number of tillerings and rhizomes, and it also promoted the elongation of rhizomes. Ethephon, ethylen-releasing compound promoted the elongation of rhizomes. Paclobutrazole, inhibitor of gibberellin-biosynthesis, promoted the increase of the number of tillerings and rhizomes, while it greatly suppressed their elongation. GA3 promoted the elongation of shoots, but inhibited the development of tillerings and rhizomes.View full abstractDownload PDF (791K) -
Yasuyuki ITO, Masanobu FUKAMI, Yoshito ASANO, Kiyoyuki SUGIURA, Masasi ...1995 Volume 23 Issue 2 Pages 128-133
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSGenetic transformation of creeping bentgrass (Agrostis palustris Hunds.) “Penncross” by electroporation-mediated direct DNA transfer to protoplasts is described. Two plasmid constructs carrying the selectable marker gene, npt II, and the truncated form of the cry I A (a) gene (the insect control protein gene of Bacillus thuringiensis var, sotto), respectively, were used for co-transformation experiments.
Protoplasts enzymatically isolated from embryogenic suspension cultures were subjected to electroporation with DNA and cultured using “agarose-beads type” culture method. Selection of protoplast-derived cell colonies with the antiobiotic, G418, yielded 112 resistant colonies from 10 independent experiments. A total of 53 resistant plants were recovered upon transferring the colonies to regeneration medium.
PCR and PCR-Southern hybridization analysis showed that all the G418 resistant regenerants tested carried the npt II gene. Expression of the transgene was detected by enzyme assay. Co-integration frequency of the npt II gene with the cry I A (a) gene appeared 24%.View full abstractDownload PDF (3289K) -
Yuriko FUJITA, Takio ICHITANI1995 Volume 23 Issue 2 Pages 134-137
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSAn acquired resistant isolate UOP393 (=IFO 32590) of Pythium vanterpoolii to metalaxyl was compared in soil for oospore germination with its parent sensitive field isolate UOP 392 (=IFO32589) . The results obtained are as follows.
(1) No significant differences were found in oospore germination in aerated steam treated field soil without plant residues between resistant isolate and its parent sensitive field isolate. But plant residues buried only promoted oospore germination of parent sensitive field isolate.
(2) In non-treated field soil, oospore germination of sensitive isolate enhanced with or without plant residues.
(3) No significant differences were found in oospore germination between resistant isolate and its parent sensitive field isolate in aerated steam treated field soil and in non-treated field soil.View full abstractDownload PDF (1955K) -
Makoto KOBAYASHI, Hiroshi NAKANO, Takayoshi TERAUCHI1995 Volume 23 Issue 2 Pages 138-140
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSNatural born seeds of St. Augustine grass (Stenotaphrum secundatum (Walt.) O. Kuntze.) were harvested and examined. Thousand kernel weight, seed length and seed width was 1.28g, 3.79mm and 1.36mm, respectively. Although seeds were treated with 0.2 % KNO3 and /or prechilling to hasten germination, significant effect could not be detected. Since harvesting pure seeds requires much time and precise work, seed propagation is not practical but applicable to reserve of genetic resources or cross breeding.View full abstractDownload PDF (3423K) -
Yoshiyasu HIGUMA1995 Volume 23 Issue 2 Pages 141-144
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSDownload PDF (400K) -
Seturo SATO1995 Volume 23 Issue 2 Pages 145-157
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSDownload PDF (1414K) -
Akio GOTO1995 Volume 23 Issue 2 Pages 158-160
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSDownload PDF (276K) -
Tatsuaki NISHIO1995 Volume 23 Issue 2 Pages 161-162
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSDownload PDF (225K) -
Humiaki MIYACHI1995 Volume 23 Issue 2 Pages 163-167
Published: March 31, 1995
Released on J-STAGE: June 08, 2010
JOURNAL FREE ACCESSDownload PDF (7612K)
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