We studied the interaction of a β-adrenergic receptor agonist and an adenylyl cyclase activator with volatile anesthetics in combination with nitrous oxide (N
2O). The whole-cell patch clamp technique was used to measure the amplitude of L-type Ca
2+ channel current(I
Ca,L) in rabbit ventricular myocytes. The effects of sevoflurane or halothane in combination with N
2O were compared with their effects in the presence of β-adrenoceptor stimulation by isoproterenol or adenylyl cyclase activation by forskolin. The effects of sevoflurane plus N
2O and halothane plus N
2O under basal conditions significantly differed from their effects in the presence of isoproterenol. Isoproterenol and forskolin diminished the ability of two gas combinations to suppress I
Ca,L. Our findings suggest that both sevoflurane and halothane in the presence of N
2O modulate the catecholamine sensitivity of the β-adrenergic cascade.
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